海马抗氧化肽在体内和体外模型中的抗光老化作用

IF 4.9 2区 医学 Q1 CHEMISTRY, MEDICINAL
Marine Drugs Pub Date : 2024-10-14 DOI:10.3390/md22100471
Fengqi Yang, Yang Yang, Dandan Xiao, Poongho Kim, Jihee Lee, You-Jin Jeon, Lei Wang
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引用次数: 0

摘要

过度暴露于紫外线(UV)辐射会导致光老化,从而造成皮肤损伤。本研究旨在评估从海马(Hippocampus abdominalis)脂肪酶水解物中纯化的抗氧化肽(SHP2)对人角质形成细胞(HaCaT)、人真皮成纤维细胞(HDF)和斑马鱼模型中紫外线照射皮肤损伤的影响。数据显示,SHP2 可通过降低 UVB 刺激的 HaCaT 细胞的细胞内活性氧(ROS)水平,减少细胞凋亡,从而显著提高细胞活力。此外,SHP2 还能有效抑制 ROS,改善 UVB 照射下 HDF 细胞中胶原蛋白的合成,抑制基质金属蛋白酶(MMPs)的分泌。SHP2 恢复了经 UVB 处理的 HDF 细胞中 HO-1、Nrf2 和 SOD 的蛋白水平,同时降低了 Keap1 的表达,这表明 Keap1/Nrf2/HO-1 信号通路受到了刺激。此外,一项在斑马鱼体内进行的研究证实,SHP2 可通过抑制 ROS 生成和脂质过氧化减少细胞死亡,从而抑制光老化。特别是,200 µg/mL 的 SHP2 对体外和体内模型都有显著的抗光老化作用。这些结果表明,SHP2 具有抗氧化特性并能调节皮肤光老化活动,这表明 SHP2 有潜力用于化妆品的开发。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anti-Photoaging Effects of Antioxidant Peptide from Seahorse (Hippocampus abdominalis) in In Vivo and In Vitro Models.

Overexposure to ultraviolet (UV) radiation can lead to photoaging, which contributes to skin damage. The objective of this study was to evaluate the effects of an antioxidant peptide (SHP2) purified from seahorse (Hippocampus abdominalis) alcalase hydrolysate on UVB-irradiated skin damage in human keratinocyte (HaCaT) and human dermal fibroblast (HDF) cells and a zebrafish model. The data revealed that SHP2 significantly enhanced cell viability by attenuating apoptosis through the reduction of intracellular reactive oxygen species (ROS) levels in UVB-stimulated HaCaT cells. Moreover, SHP2 effectively inhibited ROS, improved collagen synthesis, and suppressed the secretion of matrix metalloproteinases (MMPs) in UVB-irradiated HDF cells. SHP2 restored the protein levels of HO-1, Nrf2, and SOD, while decreasing Keap1 expression in UVB-treated HDF, indicating stimulation of the Keap1/Nrf2/HO-1 signaling pathway. Furthermore, an in vivo study conducted in zebrafish confirmed that SHP2 inhibited photoaging by reducing cell death through the suppression of ROS generation and lipid peroxidation. Particularly, 200 µg/mL of SHP2 exerted a remarkable anti-photoaging effect on both in vitro and in vivo models. These results demonstrate that SHP2 possesses antioxidant properties and regulates skin photoaging activities, suggesting that SHP2 may have the potential for use in the development of cosmetic products.

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来源期刊
Marine Drugs
Marine Drugs 医学-医药化学
CiteScore
9.60
自引率
14.80%
发文量
671
审稿时长
1 months
期刊介绍: Marine Drugs (ISSN 1660-3397) publishes reviews, regular research papers and short notes on the research, development and production of drugs from the sea. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible, particularly synthetic procedures and characterization information for bioactive compounds. There is no restriction on the length of the experimental section.
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