利用厩蝇血餐评估牛群的牛白血病病毒感染状况:一项试点研究。

IF 1.2 3区 农林科学 Q3 VETERINARY SCIENCES
Kaori Shimizu, Chikahiro Mori, Ayaka Okada, Yasuo Inoshima
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引用次数: 0

摘要

牛白血病(EBL)是一种 B 细胞淋巴瘤,在日本的发病率正在上升。EBL 由牛白血病病毒(BLV;逆转录病毒科,Deltaretrovirus bovleu)感染引起,可通过检测牛奶和血液中的 BLV 抗体或血液中的 BLV DNA 进行诊断。我们评估了使用厩蝇(Stomoxys calcitrans)作为采样工具来评估牛群中 BLV 感染状况的可行性。首先,我们采集了 3 个牛群的血液,并根据实时定量 PCR(qPCR)测定的 BLV 病毒载量(PVL),确定了 1)无 BLV 牛群;2)BLV 感染牛发病率低且 PVL 低的牛群;3)半数牛感染 BLV 且 PVL 低至高的牛群。接下来,我们从这 3 个牛群中收集稳定的苍蝇,从它们的血餐中提取 DNA,分析 BLV DNA,并测定 BLV PVL。通过消化苍蝇,我们成功检测到了牛 DNA 和 BLV DNA,但没有检测到其他哺乳动物的 DNA。根据苍蝇血餐 qPCR,我们确定一个牛群不含 BLV,另外两个牛群则有
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Use of blood meals from stable flies to evaluate the bovine leukemia virus infection status in cattle herds: a pilot study.

The incidence of enzootic bovine leukosis (EBL), a type of B-cell lymphoma, is increasing in Japan. EBL is caused by bovine leukemia virus (BLV; Retroviridae, Deltaretrovirus bovleu) infection and is diagnosed by detecting antibodies against BLV in milk and blood or BLV DNA in blood. We assessed the feasibility of using stable flies (Stomoxys calcitrans) as a sampling tool to assess BLV infection status in cattle herds. First, we collected blood from 3 cattle herds and, based on the measurement of BLV-proviral load (PVL) by quantitative real-time PCR (qPCR), identified 1) a BLV-free herd, 2) a herd with a low prevalence of BLV-infected cattle and low PVL, and 3) a herd wherein half of the cattle were BLV-infected with low-to-high PVLs. Next, we collected stable flies from the 3 herds, extracted DNA from their blood meals, analyzed it for BLV DNA, and measured the BLV PVL. Cattle DNA and BLV DNA, but not other mammalian DNA, were successfully detected by digestion of the flies. Based on fly blood meal qPCR, we identified one herd as BLV-free and the other 2 herds as having <50% prevalence of BLV-infected cattle with low PVLs. Our fly results were not consistent with preliminary BLV-PVL measurements on cattle blood. Our pilot study indicated that, to assess the feasibility of a stable fly blood meal test as an alternative technique for evaluating BLV infection status in dairy and beef cattle, additional investigations involving more cattle herds and stable flies are needed.

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来源期刊
CiteScore
3.00
自引率
6.70%
发文量
127
审稿时长
6-16 weeks
期刊介绍: The Journal of Veterinary Diagnostic Investigation (J Vet Diagn Invest) is an international peer-reviewed journal published bimonthly in English by the American Association of Veterinary Laboratory Diagnosticians (AAVLD). JVDI is devoted to all aspects of veterinary laboratory diagnostic science including the major disciplines of anatomic pathology, bacteriology/mycology, clinical pathology, epidemiology, immunology, laboratory information management, molecular biology, parasitology, public health, toxicology, and virology.
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