比较定制血糖颗粒挑战和口服糖试验,以测量马的血糖和胰岛素反应。

IF 2.6 2区 农林科学
Kristen Thane, Johanna Sonntag, Tobias Warnken, Dania Reiche, Cassandra Uricchio, Nicholas Frank
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引用次数: 0

摘要

背景:通常对马进行胰岛素失调(ID)检测,以指导管理和治疗策略:评估新开发的血糖颗粒挑战(GPC),并将结果与低剂量口服糖试验(OST)的结果进行比较:方法:随机交叉试验:方法:进行随机交叉试验。马匹接受 GPC(0.5 克升糖碳水化合物/千克体重)和 OST(0.15 毫升玉米糖浆/千克体重)试验的时间间隔为 7 天。测试前不喂食,GPC 在 T0、T60、T120 和 T180 分钟采集血样,OST 在 T0、T60 和 T90 分钟采集血样。使用床旁血糖仪测量血糖浓度,使用放射免疫分析法测量胰岛素。使用非参数检验、线性回归和 Bland-Altman 一致性分析进行比较:结果:18 匹马在 10 分钟内摄入了大于 85% 的 GPC 颗粒,且 OST 结果合格。与 OST 相比,GPC 的最大葡萄糖浓度(P = .02)和胰岛素浓度(P = .007)明显更高。达到最大胰岛素浓度的时间(Tmax[ins])在测试内部和不同测试之间存在差异,Tmax[ins](P = .28)和最大胰岛素浓度(P = .46)均与马匹服用颗粒的时间无关:结论:GPC耐受性良好,可为ID提供另一种诊断测试方式。结论:GPC耐受性良好,可为ID提供另一种诊断测试方式。GPC期间血糖和胰岛素浓度升高,达到的浓度高于低剂量OST。GPC和OST的Tmax[ins]不尽相同,这强调了确定采集样本的最佳时间范围以捕捉与诊断相关的胰岛素浓度变化的重要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of a customized glycemic pellets challenge with the oral sugar test to measure glycemic and insulinemic responses in horses.

Background: Testing for insulin dysregulation (ID) in horses is commonly performed to guide management and therapeutic strategies.

Objectives: To evaluate a newly developed glycemic pellets challenge (GPC) and compare results to those obtained using the low-dose oral sugar test (OST).

Animals: Twenty-four adult horses with unknown insulin status.

Methods: A randomized crossover trial was performed. Horses underwent GPC (0.5 g glycemic carbohydrates/kg body weight) and OST (0.15 mL corn syrup/kg body weight) 7 days apart. Feed was withheld before testing and blood samples were collected at T0, T60, T120, and T180 minutes for GPC and at T0, T60, and T90 minutes for OST. Blood glucose concentration was measured using a point-of-care glucometer and insulin by radioimmunoassay. Comparisons were made using nonparametric tests, linear regression, and Bland-Altman agreement analysis.

Results: Eighteen horses consumed >85% of the GPC pellets within 10 minutes and had acceptable OST results. Maximum glucose (P = .02) and insulin (P = .007) concentrations were significantly higher for GPC compared with OST. Time to maximum insulin concentration (Tmax[ins]) varied within and between tests and neither Tmax[ins] (P = .28) nor maximum insulin concentration (P = .46) was correlated with the time horses took to consume pellets.

Conclusions: The GPC is well tolerated and may offer another diagnostic testing modality for ID. Blood glucose and insulin concentrations increase during GPC and reach higher concentrations than observed with low-dose OST. The Tmax[ins] varied for GPC and OST, emphasizing the importance of identifying the optimal time range for the collection of samples to capture diagnostically relevant changes in insulin concentration.

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来源期刊
Journal of Veterinary Internal Medicine
Journal of Veterinary Internal Medicine Veterinary-General Veterinary
自引率
11.50%
发文量
243
期刊介绍: The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.
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