2010-2022 年塞内加尔连续 13 年流感监测后甲型 H3N2 流感病毒的基因和抗原特征。

IF 6.8 3区 医学 Q1 VIROLOGY
Mamadou Malado Jallow, Mamadou Aliou Barry, Ndiendé Koba Ndiaye, Cheikh Talibouya Touré, Cheikh Talla, Davy Kiori, Samba Niang Sagne, Sara Sy, Deborah Goudiaby, Mbayame Ndiaye Niang, Moussa Moise Diagne, Gamou Fall, Cheikh Loucoubar, Ndongo Dia
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引用次数: 0

摘要

尽管许多非洲国家对流感进行了数十年的监测,但对季节性流感病毒的进化动态却知之甚少。本研究旨在描述2010年至2022年塞内加尔A/H3N2病毒的流行病学、遗传和抗原特征。使用反转录聚合酶链反应确认了 A/H3N2 感染。随后,选择了具有代表性的 A/H3N2 分离物进行基因组测序。使用 Pepitope 模型测量了预测的疫苗效力。在研究期间,共检测了 22638 个样本,31.8% 的样本检测出流感,其中 78.1% 的样本确诊为甲型流感。在甲型流感病例中,29.8%检测到 H3N2 亚型,在雨季的预期时间达到高峰。对 123A/H3N2 分离物进行的基因组测序获得了 24 个完整基因组序列和 99 个部分基因组序列。系统发生学分析表明,塞内加尔存在多个 A/H3N2 支系,包括 2a.3、3C.2 和 3C.3a。A/H3N2 分离物主要对流感抗病毒药物奥司他韦和扎那米韦敏感,但在所有分离物中都发现了主要的金刚烷胺抗药性标记 S31N。据预测,A/H3N2 株系中至少有九个潜在的 N-连接糖基化位点,其中六个(位于 24、38、79、181、262 和 301 位)在所有分离株中保持不变。流行毒株与疫苗病毒之间的抗原性距离表明疫苗的效力各不相同,从次优到中等保护。研究结果表明,有必要加强当地的基因组和抗原监测,并进一步研究撒哈拉以南非洲地区的流感流行病学和基因演变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genetic and antigenic characterization of influenza A(H3N2) virus after 13 consecutive years of influenza surveillance in Senegal, 2010–2022

Despite decades of influenza surveillance in many African countries, little is known about the evolutionary dynamics of seasonal influenza viruses. This study aimed to characterize the epidemiological, genetic and antigenic profiles of A/H3N2 viruses in Senegal from 2010 to 2022. A/H3N2 infection was confirmed using reverse transcription-polymerase chain reaction. Subsequently, a representative of A/H3N2 isolates was selected for genome sequencing. Predicted vaccine efficacy was measured using the Pepitope model. During the study period, 22638 samples were tested and influenza was detected in 31.8%, among which type A was confirmed in 78.1%. Of the Influenza A cases, the H3N2 subtype was detected in 29.8%, peaking at expected times during the rainy season. Genome sequencing of 123A/H3N2 isolates yielded 24 complete and 99 partial genomic sequences. Phylogenetic analysis revealed the circulation of multiple clades of A/H3N2 in Senegal, including 2a.3, 3C.2 and 3C.3a. A/H3N2 isolates were mainly susceptible to the influenza antiviral drugs oseltamivir and zanamivir, but the primary adamantine-resistance marker, S31N was encountered in all isolates. At least nine potential N-linked glycosylation sites were predicted among A/H3N2 strains, six of which (at positions 24, 38, 79, 181, 262 and 301) remains conserved among all isolates. Antigenic distances between circulating strains and vaccine viruses indicated varying vaccine efficacies, from suboptimal to moderate protection. The findings emphasize the need to enhance local genomic and antigenic surveillance and further research on influenza epidemiology and genetic evolution in sub-Saharan Africa.

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来源期刊
Journal of Medical Virology
Journal of Medical Virology 医学-病毒学
CiteScore
23.20
自引率
2.40%
发文量
777
审稿时长
1 months
期刊介绍: The Journal of Medical Virology focuses on publishing original scientific papers on both basic and applied research related to viruses that affect humans. The journal publishes reports covering a wide range of topics, including the characterization, diagnosis, epidemiology, immunology, and pathogenesis of human virus infections. It also includes studies on virus morphology, genetics, replication, and interactions with host cells. The intended readership of the journal includes virologists, microbiologists, immunologists, infectious disease specialists, diagnostic laboratory technologists, epidemiologists, hematologists, and cell biologists. The Journal of Medical Virology is indexed and abstracted in various databases, including Abstracts in Anthropology (Sage), CABI, AgBiotech News & Information, National Agricultural Library, Biological Abstracts, Embase, Global Health, Web of Science, Veterinary Bulletin, and others.
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