探索从川芎中分离的内生真菌促进植物生长的特性及其在植物生长发育中的相互作用

IF 4.2 2区 生物学 Q2 MICROBIOLOGY
Qing Wang, Xinyu Zhang, Qiqi Xie, Jiwen Tao, Yujie Jia, Yirong Xiao, Zizhong Tang, Qingfeng Li, Ming Yuan, Tongliang Bu
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引用次数: 0

摘要

内生真菌栖息在各种植物组织和器官中,不会引起明显的疾病症状。它们能对植物的生长做出积极贡献,增强植物对环境和生物压力的适应能力,并促进次生代谢物的积累。这些微生物资源在各种应用中具有重要的开发和利用价值。因此,本研究重点探讨了川芎(CX)中 14 种内生真菌的植物生长促进(PGP)性状,并阐明了其促进植物生长的作用和机制。根据 PGP 活性评价,大多数菌株具有产生 IAA(78.57%)、苷元(50.00%)、氨(35.71%)、钾溶解(21.43%)、固氮(57.14%)和磷酸盐溶解(42.86%)的能力。进一步的研究表明,IAA 的含量在 13.05 至 301.43 μg/mL 之间,而可溶性磷的含量在 47.32 至 125.95 μg/mL 之间。共培养试验表明,镰刀菌 YMY5、轮枝菌 YMY6、交链孢 ZZ10 和镰刀菌 ZZ13 对烟草侧根数量和鲜重有一定的促进作用。此外,ZZ10 和 ZZ13 还能显著提高烟草种子的发芽势、发芽指数和活力指数。随后的盆栽试验表明,四种内生真菌对烟草的生长参数有一定程度的促进作用。ZZ10和ZZ13处理的促进效果最好。接种 YMY5 增加了叶绿素 a 和总叶绿素含量。ZZ10和ZZ13处理显著提高了烟草叶片的净光合速率、可溶性糖和可溶性蛋白含量、过氧化氢酶和过氧化物酶活性,降低了丙二醛含量。此外,YMY5 还能显著提高超氧化物歧化酶的活性。ZZ13 能上调生长相关基因的表达。其中,ZZ13 的促生长效果更好。总之,这些具有多性状特征和促进植物生长能力的内生真菌有望成为生物肥料或植物生长调节剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exploring Plant Growth-Promoting Traits of Endophytic Fungi Isolated from Ligusticum chuanxiong Hort and Their Interaction in Plant Growth and Development.

Endophytic fungi inhabit various plant tissues and organs without inducing evident disease symptoms. They can contribute positively to the growth of plants, bolster plants resilience to environmental and biological stresses, and facilitate the accumulation of secondary metabolites. These microbial resources possess significant developmental and utilization value in various applications. Hence, this study focused on exploring the plant growth-promoting (PGP) traits of 14 endophytic fungi from Ligusticum chuanxiong Hort (CX) and elucidating the effects and mechanisms that facilitate plant growth. According to PGP activity evaluation, the majority of strains demonstrated the capacity to produce IAA (78.57%), siderophores (50.00%), ammonia (35.71%), potassium solubilization (21.43%), nitrogen fixation (57.14%), and phosphate solubilization (42.86%). Further investigations indicated that the levels of IAA ranged from 13.05 to 301.43 μg/mL, whereas the soluble phosphorus levels ranged from 47.32 to 125.95 μg/mL. In cocultivation assays, it was indicated that Fusarium sp. YMY5, Colletotrichum sp. YMY6, Alternaria sp. ZZ10 and Fusarium sp. ZZ13 had a certain promoting effect on lateral root number and fresh weight of tobacco. Furthermore, ZZ10 and ZZ13 significantly enhanced the germination potential, germination index, and vigor index of tobacco seeds. The subsequent potted trials demonstrated that the four endophytic fungi exhibited an enhancement to growth parameters of tobacco to a certain extent. ZZ10 and ZZ13 treatment had the best promotion effect. Inoculation with YMY5 increased the chlorophyll a and total chlorophyll content. ZZ10 and ZZ13 treatment remarkably increased the net photosynthetic rate, soluble sugars and soluble protein content, catalase and peroxidase activities, and lowered malondialdehyde content in tobacco leaves. In addition, YMY5 remarkably elevated superoxide dismutase activities. ZZ13 upregulated the expression of growth-related gene. Among them, ZZ13 had a better growth-promoting effect. In conclusion, these endophytic fungi possessing multi-trait characteristics and the capacity to enhance plant growth exhibit promising potential as biofertilizers or plant growth regulators.

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来源期刊
Journal of Fungi
Journal of Fungi Medicine-Microbiology (medical)
CiteScore
6.70
自引率
14.90%
发文量
1151
审稿时长
11 weeks
期刊介绍: Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.
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