中国香港红树林中酵母菌的多样性和抗真菌敏感性--一个健康角度。

IF 4.2 2区 生物学 Q2 MICROBIOLOGY
Pak-Ting Hau, Anson Shiu, Emily Wan-Ting Tam, Eddie Chung-Ting Chau, Michaela Murillo, Eva Humer, Wai-Wai Po, Ray Chun-Wai Yu, Joshua Fung, Sai-Wang Seto, Chi-Ching Tsang, Franklin Wang-Ngai Chow
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引用次数: 0

摘要

虽然红树林生态系统具有丰富的生物多样性,但它们受到气候变化和城市污染物的影响日益严重。本研究首次揭示了香港红树林中可能出现的致病酵母菌。研究人员从十个城市和农村红树林地点采集了沉积物和水样本。初步的 CHROMagarTM Candida Plus 筛选(这是首次将这种差异培养基用于从非临床环境中采集的水和土壤样本)使从红树林中分离的酵母菌得到了快速、初步的表型鉴定。随后进行了分子剖析(ITS 和/或 28S nrDNA 测序)和抗真菌药敏试验,以进一步阐明酵母菌的多样性和耐药性。从香港红树林的沉积物和水体中分离出了多种酵母菌,包括 13 个属/支系 18 个不同物种的 45 个分离物。分子分析表明,念珠菌/巴豆菌支系(44.4%)占主导地位,这是一组臭名昭著的机会致病菌。研究结果还揭示了红树林中丰富的非念珠菌/巴豆酵母菌生物多样性,包括首次报道的 Apiotrichum domesticum 和 Crinitomyces flavificans。此外,还发现了一个潜在的新物种 Yamadazyma。值得注意的是,在无处不在的副丝状念珠菌分离物中,有 14.3% 显示出对多种抗真菌药物的耐药性,这表明红树林可能是多种药物耐药性的储存库。野生动物,尤其是候鸟,可能会传播这些隐藏的威胁。由于对病原酵母菌的环境起源、耐药性和对公共卫生的影响存在巨大的知识差距,因此需要从 "一体健康 "的角度进行紧急监测。这项研究提供了一个预警,即无节制的城市化可能会从全球沿海生态系统中释放出具有抗药性的病原体。它强调了加强监测研究以及临床医生、鸟类学家和环境微生物学家之间跨学科合作的必要性,以有效监测和管理这一环境健康风险,确保维护 "一体健康"。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Diversity and Antifungal Susceptibilities of Yeasts from Mangroves in Hong Kong, China-A One Health Aspect.

While mangrove ecosystems are rich in biodiversity, they are increasingly impacted by climate change and urban pollutants. The current study provides first insights into the emergence of potentially pathogenic yeasts in Hong Kong's mangroves. Sediment and water samples were collected from ten urban and rural mangroves sites. Initial CHROMagarTM Candida Plus screening, representing the first application of this differential medium for water and soil samples collected from a non-clinical environment, enabled the rapid, preliminary phenotypic identification of yeast isolates from mangroves. Subsequent molecular profiling (ITS and/or 28S nrDNA sequencing) and antifungal drug susceptibility tests were conducted to further elucidate yeast diversity and drug resistance. A diversity of yeasts, including 45 isolates of 18 distinct species across 13 genera/clades, was isolated from sediments and waters from Hong Kong mangroves. Molecular profiling revealed a dominance of the Candida/Lodderomyces clade (44.4%), a group of notorious opportunistic pathogens. The findings also reveal a rich biodiversity of non-Candida/Lodderomyces yeasts in mangroves, including the first reported presence of Apiotrichum domesticum and Crinitomyces flavificans. A potentially novel Yamadazyma species was also discovered. Remarkably, 14.3% of the ubiquitous Candida parapsilosis isolates displayed resistance to multiple antifungal drugs, suggesting that mangroves may be reservoirs of multi-drug resistance. Wildlife, especially migratory birds, may disseminate these hidden threats. With significant knowledge gaps regarding the environmental origins, drug resistance, and public health impacts of pathogenic yeasts, urgent surveillance is needed from a One Health perspective. This study provides an early warning that unrestrained urbanization can unleash resistant pathogens from coastal ecosystems globally. It underscores the necessity for enhanced surveillance studies and interdisciplinary collaboration between clinicians, ornithologists, and environmental microbiologists to effectively monitor and manage this environmental health risk, ensuring the maintenance of 'One Health'.

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来源期刊
Journal of Fungi
Journal of Fungi Medicine-Microbiology (medical)
CiteScore
6.70
自引率
14.90%
发文量
1151
审稿时长
11 weeks
期刊介绍: Journal of Fungi (ISSN 2309-608X) is an international, peer-reviewed scientific open access journal that provides an advanced forum for studies related to pathogenic fungi, fungal biology, and all other aspects of fungal research. The journal publishes reviews, regular research papers, and communications in quarterly issues. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on paper length. Full experimental details must be provided so that the results can be reproduced.
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