Swagatika Mishra, Pragyan P Dash, Patitapaban Mohanty, Manasa K Panda, Monalisa Mohanty, Prasant K Nanda, Santosh K Behera, Suban K Sahoo, Renjith Bhaskaran, Bigyan Ranjan Jali
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引用次数: 0
摘要
基于 2,4-二硝基苯肼的希夫碱（L）是一种有效的荧光传感器，可用于马来酸的选择性检测。使用本西-希尔德布兰德方法得出 L 与马来酸的结合比例为 1:1。测得的结合常数（Ka）为 5.17 × 106 M-1。通过使用 FT-IR、DLS 和 SEM 分析以及 DFT 计算确认了 L 的传感行为。理论评估清楚地表明，L 的单质子化和在溶剂介质中的络合是传感过程中的主要机制。此外，L 被用来对活细胞中的马来酸进行成像，这证明了它潜在的生物学用途。此外，还报告了对食品添加剂中马来酸的识别。
A Schiff-Base Molecular Probe for Selective Fluorescence Sensing of Maleic Acid with Recognition of Maleic Acid in Food Additives and Cell Imaging.
The 2,4-dinitrophenylhydrazine based Schiff base (L) acts as an effective fluorescence sensor for the selective detection of maleic acid. The detection limit of L towards maleic acid is observed to be 1.29 × 10-7 M. A 1:1 binding stoichiometry between L and maleic acid was obtained using Bensi-Hilderbrand method. The binding constant (Ka) was measured to be 5.17 × 106 M-1. The sensing behavior of L was confirmed through analysis using FT-IR, DLS and SEM analysis, alongside DFT calculations. Theoretical assessments clearly suggest that the L's mono-protonation and complexation in the solvent medium are the primary mechanisms in the sensing process. Additionally, L is used to imaging the maleic acid in living cells, demonstrating its potential biological uses. In addition, recognition of maleic acid in food additives was reported.
期刊介绍：
Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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