低能量体外冲击波疗法可改善大鼠脊髓损伤模型的运动功能和组织再生,并调节炎症诱导的 FGF1 和 FGF2 信号传导,从而保护受损组织:一项动物实验研究。

IF 12.5 2区 医学 Q1 SURGERY
Chieh-Cheng Hsu, Kay Lh Wu, Jei-Ming Peng, Yi-No Wu, Hou-Tsung Chen, Meng-Shiou Lee, Jai-Hong Cheng
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引用次数: 0

摘要

背景:脊髓损伤(SCI)是一种使人衰弱的疾病,会导致严重的运动功能障碍。目前的治疗方案仍然有限,这凸显了对新型疗法的需求。体外冲击波疗法(ESWT)已成为治疗肌肉骨骼疾病和神经再生的一种很有前景的非侵入性方法:本研究探讨了低能量 ESWT 对大鼠 SCI 模型的运动功能、组织再生、炎症和线粒体功能的影响。实验采用了运动功能测试、CatWalk步态分析、组织病理学检查、免疫组织化学和免疫荧光染色等方法:结果:研究结果表明,低能量 ESWT 具有剂量依赖效应,与单次治疗相比,三次治疗(ESWT3)的效果更佳。ESWT3 能明显改善运动功能(跑步模式、跑步平均速度和最大变化,以及巴索、比提和布雷斯纳汉 (BBB) 评分),促进组织再生,同时减少炎症。ESWT3 能明显降低 IL-1β、IL6 和巨噬细胞(CD68)的水平,同时增加白细胞(CD45)的浸润。此外,ESWT3 还能上调 NueN 和 mitofusin 2 (MFN2),这表明神经元健康和线粒体功能得到了增强。此外,ESWT3还能调节成纤维细胞生长因子1(FGF1)、FGF2及其受体FGFR1的表达和ERK的磷酸化,有助于SCI的组织修复和再生:本研究强调了低能量 ESWT 作为一种有效的非侵入性 SCI 治疗方法的潜力,它在运动恢复、组织再生、抗炎作用和线粒体保护方面均有显著改善。这些发现为了解 ESWT 的机制及其在 SCI 恢复中的治疗应用提供了宝贵的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Low-Energy extracorporeal shockwave therapy improves locomotor functions, tissue regeneration and modulating the inflammation induced FGF1 and FGF2 signaling to protect damaged tissue in spinal cord injury of rat model: An experimental animal study.

Background: Spinal cord injury (SCI) is a debilitating condition that results in severe motor function impairments. Current therapeutic options remain limited, underscoring the need for novel treatments. Extracorporeal shockwave therapy (ESWT) has emerged as a promising noninvasive approach for treating musculoskeletal disorders and nerve regeneration.

Methods: This study explored the effects of low-energy ESWT on locomotor function, tissue regeneration, inflammation, and mitochondrial function in a rat SCI model. Experiments were performed using locomotor function assays, CatWalk gait analysis, histopathological examination, immunohistochemical and immunofluorescence staining.

Results: The findings demonstrated that low-energy ESWT had a dose-dependent effect, with three treatment sessions (ESWT3) showing superior outcomes compared to a single session. ESWT3 significantly improved motor functions (run patterns, run average speed, and maximum variation, as well as the Basso, Beattie, and Bresnahan (BBB) score) and promoted tissue regeneration while reducing inflammation. ESWT3 significantly decreased levels of IL-1β, IL6 and macrophages (CD68) while increasing leucocyte (CD45) infiltration. Additionally, ESWT3 upregulated NueN and mitofusin 2 (MFN2), suggesting enhanced neuronal health and mitochondrial function. Moreover, ESWT3 modulated the expression of fibroblast growth factor 1 (FGF1), FGF2, their receptor FGFR1 and phosphorylation of ERK, aiding tissue repair and regeneration in SCI.

Conclusions: This study highlights the potential of low-energy ESWT as an effective noninvasive treatment for SCI, demonstrating significant improvements in motor recovery, tissue regeneration, anti-inflammatory effects, and mitochondrial protection. These findings provide valuable insights into the mechanisms of ESWT and its therapeutic application for SCI recovery.

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来源期刊
CiteScore
17.70
自引率
3.30%
发文量
0
审稿时长
6-12 weeks
期刊介绍: The International Journal of Surgery (IJS) has a broad scope, encompassing all surgical specialties. Its primary objective is to facilitate the exchange of crucial ideas and lines of thought between and across these specialties.By doing so, the journal aims to counter the growing trend of increasing sub-specialization, which can result in "tunnel-vision" and the isolation of significant surgical advancements within specific specialties.
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