{"title":"尼泊尔加德满都人类、食物和环境中产广谱β-内酰胺酶(ESBL)大肠埃希菌的流行情况:ESBL大肠杆菌三循环项目的研究结果。","authors":"Jyoti Acharya, Runa Jha, Tulsi Ram Gompo, Sharmila Chapagain, Lilee Shrestha, Nisha Rijal, Anjana Shrestha, Pragya Koirala, Suraj Subedi, Binita Tamang, Hari Prasad Kattel, Bishal Khaniya, Basudha Shrestha, Aruna Karki, Ram Prasad Adhikari, Sanita Kayastha, Prasil Pradhan, Sarada Duwal Shrestha, Bijendra Raj Raghubanshi, Heera Tuladhar, Palpasa Kansakar, Saugat Shrestha, Priyanka Shrestha, Binay Shrestha, Ricardo J Soares Magalhaes, Manish Kakkar, Arunkumar Govindakarnavar, Allison Gocotano, Reuben Samuel","doi":"10.1155/2024/1094816","DOIUrl":null,"url":null,"abstract":"<p><p>The need to address antimicrobial resistance (AMR) through a One Health (OH) approach is now well recognized. There is, however, limited guidance on how AMR surveillance should be implemented across sectors to generate meaningful AMR and AMU data for decision-making. Using a sympatric approach to cross-sector sample collection, Nepal adopted the WHO extended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i> (<i>E. coli</i>) Tricycle Project as a step toward OH surveillance for assessing the prevalence of ESBL-producing <i>E. coli</i> across human, veterinary, and environment sectors. This involved a three-stage approach: identification of human hotspots (Stage 1) and sample collection sites for poultry (Stage 2) and wastewater (Stage 3). A total of 53 blood cultures from patients with bloodstream infections (BSIs), 100 stool samples from healthy pregnant women, 220 poultry ceca from slaughterhouses and live markets, and 48 wastewater samples were processed for bacterial culture and analyzed for the presence of ESBL-producing <i>E. coli</i>. The prevalence of ESBL-producing <i>E. coli</i> among isolated <i>E. coli</i> was the highest in wastewater samples (91%) followed by human BSIs (49%), poultry (38.6%), and fecal carriage isolates from healthy pregnant females (15%). A statistically significant association was seen in the prevalence of multidrug resistance among ESBL producers (52%) and nonproducers (26%). ESBL-producing <i>E. coli</i> was detected in all wastewater samples tested except for the upstream river. The findings of the study showed a high prevalence of ESBL-producing <i>E. coli</i> in samples from all three sectors and provided baseline data based upon which strategies for the safe disposal of communal and hospital waste, integrated AMR surveillance, and control strategies could be planned and implemented.</p>","PeriodicalId":14098,"journal":{"name":"International Journal of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11498988/pdf/","citationCount":"0","resultStr":"{\"title\":\"Prevalence of Extended-Spectrum Beta-Lactamase (ESBL)-Producing <i>Escherichia coli</i> in Humans, Food, and Environment in Kathmandu, Nepal: Findings From ESBL <i>E. coli</i> Tricycle Project.\",\"authors\":\"Jyoti Acharya, Runa Jha, Tulsi Ram Gompo, Sharmila Chapagain, Lilee Shrestha, Nisha Rijal, Anjana Shrestha, Pragya Koirala, Suraj Subedi, Binita Tamang, Hari Prasad Kattel, Bishal Khaniya, Basudha Shrestha, Aruna Karki, Ram Prasad Adhikari, Sanita Kayastha, Prasil Pradhan, Sarada Duwal Shrestha, Bijendra Raj Raghubanshi, Heera Tuladhar, Palpasa Kansakar, Saugat Shrestha, Priyanka Shrestha, Binay Shrestha, Ricardo J Soares Magalhaes, Manish Kakkar, Arunkumar Govindakarnavar, Allison Gocotano, Reuben Samuel\",\"doi\":\"10.1155/2024/1094816\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The need to address antimicrobial resistance (AMR) through a One Health (OH) approach is now well recognized. There is, however, limited guidance on how AMR surveillance should be implemented across sectors to generate meaningful AMR and AMU data for decision-making. Using a sympatric approach to cross-sector sample collection, Nepal adopted the WHO extended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i> (<i>E. coli</i>) Tricycle Project as a step toward OH surveillance for assessing the prevalence of ESBL-producing <i>E. coli</i> across human, veterinary, and environment sectors. This involved a three-stage approach: identification of human hotspots (Stage 1) and sample collection sites for poultry (Stage 2) and wastewater (Stage 3). A total of 53 blood cultures from patients with bloodstream infections (BSIs), 100 stool samples from healthy pregnant women, 220 poultry ceca from slaughterhouses and live markets, and 48 wastewater samples were processed for bacterial culture and analyzed for the presence of ESBL-producing <i>E. coli</i>. The prevalence of ESBL-producing <i>E. coli</i> among isolated <i>E. coli</i> was the highest in wastewater samples (91%) followed by human BSIs (49%), poultry (38.6%), and fecal carriage isolates from healthy pregnant females (15%). A statistically significant association was seen in the prevalence of multidrug resistance among ESBL producers (52%) and nonproducers (26%). ESBL-producing <i>E. coli</i> was detected in all wastewater samples tested except for the upstream river. The findings of the study showed a high prevalence of ESBL-producing <i>E. coli</i> in samples from all three sectors and provided baseline data based upon which strategies for the safe disposal of communal and hospital waste, integrated AMR surveillance, and control strategies could be planned and implemented.</p>\",\"PeriodicalId\":14098,\"journal\":{\"name\":\"International Journal of Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-10-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11498988/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1155/2024/1094816\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2024/1094816","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Prevalence of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli in Humans, Food, and Environment in Kathmandu, Nepal: Findings From ESBL E. coli Tricycle Project.
The need to address antimicrobial resistance (AMR) through a One Health (OH) approach is now well recognized. There is, however, limited guidance on how AMR surveillance should be implemented across sectors to generate meaningful AMR and AMU data for decision-making. Using a sympatric approach to cross-sector sample collection, Nepal adopted the WHO extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) Tricycle Project as a step toward OH surveillance for assessing the prevalence of ESBL-producing E. coli across human, veterinary, and environment sectors. This involved a three-stage approach: identification of human hotspots (Stage 1) and sample collection sites for poultry (Stage 2) and wastewater (Stage 3). A total of 53 blood cultures from patients with bloodstream infections (BSIs), 100 stool samples from healthy pregnant women, 220 poultry ceca from slaughterhouses and live markets, and 48 wastewater samples were processed for bacterial culture and analyzed for the presence of ESBL-producing E. coli. The prevalence of ESBL-producing E. coli among isolated E. coli was the highest in wastewater samples (91%) followed by human BSIs (49%), poultry (38.6%), and fecal carriage isolates from healthy pregnant females (15%). A statistically significant association was seen in the prevalence of multidrug resistance among ESBL producers (52%) and nonproducers (26%). ESBL-producing E. coli was detected in all wastewater samples tested except for the upstream river. The findings of the study showed a high prevalence of ESBL-producing E. coli in samples from all three sectors and provided baseline data based upon which strategies for the safe disposal of communal and hospital waste, integrated AMR surveillance, and control strategies could be planned and implemented.
期刊介绍:
International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.