尼泊尔加德满都人类、食物和环境中产广谱β-内酰胺酶(ESBL)大肠埃希菌的流行情况:ESBL大肠杆菌三循环项目的研究结果。

IF 2.8 Q3 MICROBIOLOGY
International Journal of Microbiology Pub Date : 2024-10-16 eCollection Date: 2024-01-01 DOI:10.1155/2024/1094816
Jyoti Acharya, Runa Jha, Tulsi Ram Gompo, Sharmila Chapagain, Lilee Shrestha, Nisha Rijal, Anjana Shrestha, Pragya Koirala, Suraj Subedi, Binita Tamang, Hari Prasad Kattel, Bishal Khaniya, Basudha Shrestha, Aruna Karki, Ram Prasad Adhikari, Sanita Kayastha, Prasil Pradhan, Sarada Duwal Shrestha, Bijendra Raj Raghubanshi, Heera Tuladhar, Palpasa Kansakar, Saugat Shrestha, Priyanka Shrestha, Binay Shrestha, Ricardo J Soares Magalhaes, Manish Kakkar, Arunkumar Govindakarnavar, Allison Gocotano, Reuben Samuel
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引用次数: 0

摘要

通过 "一个健康"(OH)方法解决抗菌药物耐药性(AMR)问题的必要性现已得到广泛认可。然而,在如何跨部门实施抗菌药物耐药性监测,以便为决策提供有意义的抗菌药物耐药性和抗菌药物不良反应数据方面,指导却十分有限。尼泊尔采用一种跨部门样本收集的共生方法,采纳了世界卫生组织的产扩展广谱β-内酰胺酶(ESBL)大肠埃希菌(E. coli)三周期项目,作为实现 "统一卫生 "监测的一个步骤,以评估产 ESBL 大肠埃希菌在人类、兽医和环境部门的流行情况。该项目分为三个阶段:确定人类热点(第 1 阶段)以及家禽(第 2 阶段)和废水(第 3 阶段)样本采集点。共对 53 份来自血流感染(BSI)患者的血液培养物、100 份来自健康孕妇的粪便样本、220 份来自屠宰场和活禽市场的家禽盲肠样本以及 48 份废水样本进行了细菌培养处理,并分析其中是否存在产 ESBL 大肠杆菌。在分离出的大肠杆菌中,产 ESBL 大肠杆菌在废水样本中的流行率最高(91%),其次是人类 BSIs(49%)、家禽(38.6%)和来自健康孕妇的粪便携带分离物(15%)。ESBL产生者(52%)和非产生者(26%)的多药耐药性发生率在统计学上有明显关联。除上游河流外,在检测的所有废水样本中都检测到了产 ESBL 大肠杆菌。研究结果表明,在所有三个部门的样本中,产 ESBL 大肠杆菌的流行率都很高,并提供了基线数据,可据此规划和实施公共和医院废物安全处置战略、AMR 综合监测和控制策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Prevalence of Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli in Humans, Food, and Environment in Kathmandu, Nepal: Findings From ESBL E. coli Tricycle Project.

The need to address antimicrobial resistance (AMR) through a One Health (OH) approach is now well recognized. There is, however, limited guidance on how AMR surveillance should be implemented across sectors to generate meaningful AMR and AMU data for decision-making. Using a sympatric approach to cross-sector sample collection, Nepal adopted the WHO extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (E. coli) Tricycle Project as a step toward OH surveillance for assessing the prevalence of ESBL-producing E. coli across human, veterinary, and environment sectors. This involved a three-stage approach: identification of human hotspots (Stage 1) and sample collection sites for poultry (Stage 2) and wastewater (Stage 3). A total of 53 blood cultures from patients with bloodstream infections (BSIs), 100 stool samples from healthy pregnant women, 220 poultry ceca from slaughterhouses and live markets, and 48 wastewater samples were processed for bacterial culture and analyzed for the presence of ESBL-producing E. coli. The prevalence of ESBL-producing E. coli among isolated E. coli was the highest in wastewater samples (91%) followed by human BSIs (49%), poultry (38.6%), and fecal carriage isolates from healthy pregnant females (15%). A statistically significant association was seen in the prevalence of multidrug resistance among ESBL producers (52%) and nonproducers (26%). ESBL-producing E. coli was detected in all wastewater samples tested except for the upstream river. The findings of the study showed a high prevalence of ESBL-producing E. coli in samples from all three sectors and provided baseline data based upon which strategies for the safe disposal of communal and hospital waste, integrated AMR surveillance, and control strategies could be planned and implemented.

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来源期刊
CiteScore
7.90
自引率
0.00%
发文量
57
审稿时长
13 weeks
期刊介绍: International Journal of Microbiology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on microorganisms and their interaction with hosts and the environment. The journal covers all microbes, including bacteria, fungi, viruses, archaea, and protozoa. Basic science will be considered, as well as medical and applied research.
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