早产儿出生后第一天肠道和咽部微生物群的比较,以及剖腹产或阴道分娩婴儿咽部微生物群的特点。

IF 2.1 3区 医学 Q2 PEDIATRICS
Frontiers in Pediatrics Pub Date : 2024-10-08 eCollection Date: 2024-01-01 DOI:10.3389/fped.2024.1411887
Jing He, Lijuan Wang, Ying Ruan, Xinyan Yan, Qingju Liu, Boren Chen, Sen Yang, Lijun Du
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引用次数: 0

摘要

背景:本研究旨在探讨早产儿出生第一天肠道和咽部微生物群的分布情况,并比较剖宫产儿和阴道分娩儿的微生物群组成:本研究包括 44 名胎龄为 34-36+6 周的晚期早产儿。在早产儿出生后的第一天采集其粪便和咽拭子样本。婴儿分为剖宫产组和阴道分娩组。采用 Illumina NovaSeq 高通量测序技术对样本中所有细菌的 16S rRNA 基因 V3-V4 超变区进行测序。利用维恩图确定了肠道和咽喉中共有的操作分类单元(OTU)。结果:(1)妊娠年龄可能对出生后第一天早产儿肠道和咽部的微生物定植有显著影响(p ≤ 0.001)。(2)在咽部检测到的 OTU 多于肠道,两者共有 819 个共用 OTU。咽部和肠道都有 819 个共用 OTU。在属一级,粪便样本中链球菌的相对丰度(0.5%)低于喉咙样本(0.5% 对 22.2%,p = 0.003)。3)剖宫产组咽部样本中链球菌的相对丰富度为 26.2%,远高于阴道分娩组的 3.8%(P = 0.01):结论:产后早期是建立婴儿微生物群的关键时期。出生时的胎龄可能会影响微生物定植,而出生体重、性别和分娩方式则不会。早产儿出生后第一天的肠道和咽部微生物群组成显示出高度的相似性,但需要更大的样本来进一步验证。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of intestinal and pharyngeal microbiota in preterm infants on the first day of life and the characteristics of pharyngeal microbiota in infants delivered by cesarean section or vaginally.

Background: This study aimed to explore the distribution of intestinal and pharyngeal microbiota on the first day of life in preterm infants and compare the composition of microbiota in infants delivered by cesarean section or vaginally.

Methods: This study included 44 late preterm infants with a gestational age of 34-36 + 6 weeks. Stool and throat swab samples were collected from the preterm infants on the first day of life. The infants were divided into cesarean section and vaginal delivery groups. Illumina NovaSeq high-throughput sequencing technology was used to sequence the V3-V4 hypervariable region of the 16S rRNA gene of all bacteria in the samples. Venn diagram was used to identify shared operational taxonomic units (OTUs) in the intestines and pharynges. Microbial analysis was conducted at the phylum and genus levels, and α and β diversity comparisons were performed.

Results: (1) Gestational age may have significantly affected the microbial colonization of the intestines and pharynges of preterm infants on the first day after birth (p ≤ 0.001). (2) More OTUs were detected in the pharynx than in the intestines, both have a total of 819 shared OTUs. Proteobacteria, Firmicutes, and Bacteroidota were the dominant phyla in both. At the genus level, Streptococcus had a lower relative abundance in stool samples (0.5%) compared to throat samples (0.5% vs. 22.2%, p = 0.003). 3) The relative abundance of Streptococcus in pharyngeal samples was 26.2% in the cesarean section group much higher than the 3.8% in the vaginal delivery group (p = 0.01).

Conclusion: The early postnatal period is a critical time for the establishment of an infant's microbiota. Gestational age at birth may influence microbial colonization, while birth weight, gender, and mode of delivery do not. The intestinal and pharyngeal microbiota composition of preterm infants on the first day after birth showed high similarity, but larger samples are needed for further validation.

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来源期刊
Frontiers in Pediatrics
Frontiers in Pediatrics Medicine-Pediatrics, Perinatology and Child Health
CiteScore
3.60
自引率
7.70%
发文量
2132
审稿时长
14 weeks
期刊介绍: Frontiers in Pediatrics (Impact Factor 2.33) publishes rigorously peer-reviewed research broadly across the field, from basic to clinical research that meets ongoing challenges in pediatric patient care and child health. Field Chief Editors Arjan Te Pas at Leiden University and Michael L. Moritz at the Children''s Hospital of Pittsburgh are supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. Frontiers in Pediatrics also features Research Topics, Frontiers special theme-focused issues managed by Guest Associate Editors, addressing important areas in pediatrics. In this fashion, Frontiers serves as an outlet to publish the broadest aspects of pediatrics in both basic and clinical research, including high-quality reviews, case reports, editorials and commentaries related to all aspects of pediatrics.
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