具有多重生物活性的 BioMOF@cellulose 甘油凝胶支架:骨组织修复的前景。

IF 5 3区 化学 Q1 POLYMER SCIENCE
Gels Pub Date : 2024-09-30 DOI:10.3390/gels10100631
Albert Rosado, Alejandro Borrás, Miguel Sánchez-Soto, Magdaléna Labíková, Hubert Hettegger, Rosa Ana Ramírez-Jiménez, Luís Rojo, Luís García-Fernández, María Rosa Aguilar, Falk Liebner, Ana M López-Periago, José A Ayllón, Concepción Domingo
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引用次数: 0

摘要

开发用于肌肉骨骼组织修复的新型生物材料是目前生物医学研究的一个重要分支。本文介绍的方法主要是开发一种用于骨再生的原型合成甘油凝胶支架,同时具有治疗活性。这项工作的主要创新点在于将开放式中孔和大孔纳米结晶纤维素(NCC)基甘油凝胶与由钙离子和丁香酸组成的完全生物相容性微孔生物MOF 系统(CaSyr-1)相结合。生物MOF 框架进一步浸渍了第三种生物活性成分,即布洛芬(ibu),从而产生了一种多重生物活性系统。集成的 CaSyr-1(ibu)是生物活性化合物递送的储存库,而 NCC 支架则是细胞生长、增殖和分化的拟议基质。在开氏 310 度的磷酸盐缓冲盐溶液中测量的给药曲线表明,生物活性成分在生物MOF 溶解约 30 分钟后与假性第一溶解同时释放。30 分钟后,生物活性成分与 bioMOF 的溶解同时释放,释放过程遵循伪一阶动力学模型。此外,根据半经验 Korsmeyer-Peppas 动力学模型,生物活性成分的释放受第二种情况机制的支配,这表明分子运输受到 NCC 基质松弛的影响。初步的体外实验结果表明,NCC 支架中初始的高浓度甘油在与人类成骨细胞(HObs)直接接触时会产生毒性。然而,当该系统中过量的甘油被稀释后(实验第二天后),直接和间接检测结果证实了其完全的生物相容性和对 HOb 增殖的适宜性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
BioMOF@cellulose Glycerogel Scaffold with Multifold Bioactivity: Perspective in Bone Tissue Repair.

The development of new biomaterials for musculoskeletal tissue repair is currently an important branch in biomedicine research. The approach presented here is centered around the development of a prototypic synthetic glycerogel scaffold for bone regeneration, which simultaneously features therapeutic activity. The main novelty of this work lies in the combination of an open meso and macroporous nanocrystalline cellulose (NCC)-based glycerogel with a fully biocompatible microporous bioMOF system (CaSyr-1) composed of calcium ions and syringic acid. The bioMOF framework is further impregnated with a third bioactive component, i.e., ibuprofen (ibu), to generate a multifold bioactive system. The integrated CaSyr-1(ibu) serves as a reservoir for bioactive compounds delivery, while the NCC scaffold is the proposed matrix for cell ingrowth, proliferation and differentiation. The measured drug delivery profiles, studied in a phosphate-buffered saline solution at 310 K, indicate that the bioactive components are released concurrently with bioMOF dissolution after ca. 30 min following a pseudo-first-order kinetic model. Furthermore, according to the semi-empirical Korsmeyer-Peppas kinetic model, this release is governed by a case-II mechanism, suggesting that the molecular transport is influenced by the relaxation of the NCC matrix. Preliminary in vitro results denote that the initial high concentration of glycerol in the NCC scaffold can be toxic in direct contact with human osteoblasts (HObs). However, when the excess of glycerol is diluted in the system (after the second day of the experiment), the direct and indirect assays confirm full biocompatibility and suitability for HOb proliferation.

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来源期刊
Gels
Gels POLYMER SCIENCE-
CiteScore
4.70
自引率
19.60%
发文量
707
审稿时长
11 weeks
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