Adnan Hodžić, Gorana Veinović, Amer Alić, David Seki, Martin Kunert, Georgi Nikolov, Ratko Sukara, Jovana Šupić, Snežana Tomanović, David Berry
{"title":"一种由环境获得的肠道细菌分泌的金属蛋白酶阻碍了阿夫泽尔氏包柔氏菌(Borrelia afzelii)在蓖麻线虫体内的定植。","authors":"Adnan Hodžić, Gorana Veinović, Amer Alić, David Seki, Martin Kunert, Georgi Nikolov, Ratko Sukara, Jovana Šupić, Snežana Tomanović, David Berry","doi":"10.3389/fcimb.2024.1476266","DOIUrl":null,"url":null,"abstract":"<p><p>Although the importance of the microbiome in the context of tick biology and vector competence has recently come into a broader research focus, the field is still in its infancy and the complex ecological interactions between the tick residential bacteria and pathogens are obscure. Here, we show that an environmentally acquired gut bacterium has the potential to impair <i>Borrelia afzelii</i> colonization within the tick vector through a secreted metalloprotease. Oral introduction of either <i>Bacillus cereus</i> LTG-1 isolate or its purified enhancin (<i>Bc</i>Enhancin) protein significantly reduces <i>B. afzelii</i> burden in the guts of <i>Ixodes ricinus</i> ticks. This effect is attributed to the ability of <i>Bc</i>Enhancin to degrade a glycan-rich peritrophic matrix (PM), which is a gut protective barrier essential for <i>Borrelia</i> survival. Our study highlights the importance of the gut microbiome in determining tick vector competence and provides a deeper mechanistic insight into the complex network of interactions between <i>Borrelia</i>, the tick, and the tick microbiome.</p>","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499241/pdf/","citationCount":"0","resultStr":"{\"title\":\"A metalloprotease secreted by an environmentally acquired gut bacterium hinders <i>Borrelia afzelii</i> colonization in <i>Ixodes ricinus</i>.\",\"authors\":\"Adnan Hodžić, Gorana Veinović, Amer Alić, David Seki, Martin Kunert, Georgi Nikolov, Ratko Sukara, Jovana Šupić, Snežana Tomanović, David Berry\",\"doi\":\"10.3389/fcimb.2024.1476266\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Although the importance of the microbiome in the context of tick biology and vector competence has recently come into a broader research focus, the field is still in its infancy and the complex ecological interactions between the tick residential bacteria and pathogens are obscure. Here, we show that an environmentally acquired gut bacterium has the potential to impair <i>Borrelia afzelii</i> colonization within the tick vector through a secreted metalloprotease. Oral introduction of either <i>Bacillus cereus</i> LTG-1 isolate or its purified enhancin (<i>Bc</i>Enhancin) protein significantly reduces <i>B. afzelii</i> burden in the guts of <i>Ixodes ricinus</i> ticks. This effect is attributed to the ability of <i>Bc</i>Enhancin to degrade a glycan-rich peritrophic matrix (PM), which is a gut protective barrier essential for <i>Borrelia</i> survival. Our study highlights the importance of the gut microbiome in determining tick vector competence and provides a deeper mechanistic insight into the complex network of interactions between <i>Borrelia</i>, the tick, and the tick microbiome.</p>\",\"PeriodicalId\":12458,\"journal\":{\"name\":\"Frontiers in Cellular and Infection Microbiology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-10-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499241/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Frontiers in Cellular and Infection Microbiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.3389/fcimb.2024.1476266\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"IMMUNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Cellular and Infection Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fcimb.2024.1476266","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
A metalloprotease secreted by an environmentally acquired gut bacterium hinders Borrelia afzelii colonization in Ixodes ricinus.
Although the importance of the microbiome in the context of tick biology and vector competence has recently come into a broader research focus, the field is still in its infancy and the complex ecological interactions between the tick residential bacteria and pathogens are obscure. Here, we show that an environmentally acquired gut bacterium has the potential to impair Borrelia afzelii colonization within the tick vector through a secreted metalloprotease. Oral introduction of either Bacillus cereus LTG-1 isolate or its purified enhancin (BcEnhancin) protein significantly reduces B. afzelii burden in the guts of Ixodes ricinus ticks. This effect is attributed to the ability of BcEnhancin to degrade a glycan-rich peritrophic matrix (PM), which is a gut protective barrier essential for Borrelia survival. Our study highlights the importance of the gut microbiome in determining tick vector competence and provides a deeper mechanistic insight into the complex network of interactions between Borrelia, the tick, and the tick microbiome.
期刊介绍:
Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.