表达大口鲈鱼病毒 MCP 的重组植物乳杆菌对大口鲈鱼的免疫保护作用。

IF 4.1 2区农林科学 Q1 FISHERIES

Fish & shellfish immunology Pub Date : 2024-10-24 DOI:10.1016/j.fsi.2024.109986

Yong-Lei Yang , Rong-Rong Zhang , Jing-Yi Pang , Jun-Hong Xing , Tian-Kui Guo , Chun-Wei Shi , Gui-Lian Yang , Hai-Bin Huang , Yan-Long Jiang , Jian-Zhong Wang , Xin Cao , Nan Wang , Yan Zeng , Wen-Tao Yang , Jia-Yun Yao , Chun-Feng Wang

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引用次数: 0

摘要

大口鲈鱼病毒（LMBV）是一种传染性病原体，会导致大口鲈鱼的高死亡率，这种病毒的爆发会严重危害水产养殖业。目前，尚未开发出能有效预防 LMBV 传播的疫苗。在本研究中，我们构建了一种重组植物乳杆菌（L. plantarum）菌株，该菌株能够表达 LMBV 的 MCP 基因并在其表面显示该蛋白；然后，我们评估了该重组菌对大口鲈鱼的免疫保护作用。Western 印迹、免疫荧光和流式细胞术证实，MCP 已成功表达并固定在 NC8 细胞表面。通过口服途径用 NC8-pSIP409-pgsA'-MCP 免疫大口鲈，可诱导 CD4、CD8、IL-1β 和 IL-6 基因表达。此外，不同CFU的NC8-pSIP409-pgsA'-MCP可提高大口鲈感染LMBV后的存活率，尤其是NC8-pSIP409-pgsA'-MCP（109 CFU）可提高约30%的存活率。NC8-pSIP409-pgsA'-MCP 免疫可减轻肝脏和脾脏的病理变化，发挥更有利的保护作用。这些数据表明，重组植物酵母菌株 NC8-pSIP409-pgsA'-MCP 可增强大口鲈对 LMBV 感染的抵抗力，该菌株有望成为预防 LMBV 感染的候选口服疫苗。

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Immunoprotective effect of recombinant Lactobacillus plantarum expressing largemouth bass virus MCP on largemouth bass

Largemouth bass virus (LMBV) is an infectious pathogen that causes high mortality rates in largemouth bass, and outbreaks of this virus can significantly harm the aquaculture industry. Currently, no vaccine has been developed that can effectively prevent the transmission of LMBV. In this study, we constructed a recombinant Lactobacillus plantarum (L. plantarum) strain capable of expressing the MCP gene of LMBV and displaying this protein on its surface; then, we evaluated the immunoprotective effect of this recombinant bacterium on largemouth bass. Western blotting, immunofluorescence, and flow cytometry confirmed that MCP was successfully expressed and anchored on the surfaces of NC8 cells. Immunization of largemouth bass with NC8-pSIP409-pgsA′-MCP via the oral feeding route induced CD4, CD8, IL-1β, and IL-6 gene expression. In addition, NC8-pSIP409-pgsA′-MCP at different CFUs increased the survival of largemouth bass after LMBV infection; in particular, NC8-pSIP409-pgsA′-MCP (10⁹ CFU) resulted in approximately 30 % survival. NC8-pSIP409-pgsA′-MCP immunization alleviated the pathological changes in the liver and spleen, exerting a more advantageous protective effect. These data suggest that the recombinant L. plantarum strain NC8-pSIP409-pgsA′-MCP can increase the resistance of largemouth bass to LMBV infection and that this strain is a promising candidate oral vaccine for the prevention of LMBV infection.

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来源期刊

Fish & shellfish immunology 农林科学-海洋与淡水生物学

CiteScore

7.50

自引率

19.10%

发文量

750

审稿时长

68 days

期刊介绍： Fish and Shellfish Immunology rapidly publishes high-quality, peer-refereed contributions in the expanding fields of fish and shellfish immunology. It presents studies on the basic mechanisms of both the specific and non-specific defense systems, the cells, tissues, and humoral factors involved, their dependence on environmental and intrinsic factors, response to pathogens, response to vaccination, and applied studies on the development of specific vaccines for use in the aquaculture industry.