通过毛细管电泳结合质谱法测定蚕蛾幼虫体内环丙沙星的方法验证

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Magdalena Czuma-Pokusa, Maria Walczak
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引用次数: 0

摘要

从昆虫中提取的物质具有多种生物特性,可以发挥治疗作用。本文重点介绍蚕食蝇(Lucilia sericata)这一物种,以及幼虫疗法对因住院而出现压疮和其他难以愈合伤口的患者的益处。幼虫疗法又称蛆虫清创疗法,是利用无菌苍蝇幼虫通过酶降解坏死组织和减少细菌定植来治疗慢性不愈合伤口。幼虫会在伤口上停留 48-72 小时,在此期间,它们会有效清洁伤口并刺激组织再生。这种治疗方法对顽固性伤口(如糖尿病足溃疡和压疮)特别有效,因为这些伤口对传统治疗方法没有反应。在昆虫毒理学研究中,幼虫也可作为一种替代材料,用于检测摄入的物质不仅具有毒性,还具有治疗作用。本研究介绍了一种利用毛细管电泳和质谱联用技术检测蚕蛾幼虫体内环丙沙星含量的方法。该方法灵敏度高,定量限为 100 ± 0.018 ng/mL,准确度和精密度分别为 87%-103% 和 1%-4% 。采用简单快速的蛋白质沉淀法清洗样品,分析物的回收率非常令人满意(90%-104%)。该方法在 100-1000 ng/mL 范围内线性良好,测定系数大于 0.9973。使用该方法测定了患者在使用抗生素后幼虫匀浆中的环丙沙星含量,抗生素剂量为 500 毫克,每天两次,每次口服,在使用幼虫敷料期间进行。结果表明,环丙沙星从患者血液循环分布到幼虫体内的浓度为 150 纳克/毫升(750 纳克/克)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Method Validation for the Determination of Ciprofloxacin in Lucilia sericata Larvae via Capillary Electrophoresis Combined With Mass Spectrometry

Substances derived from insects can serve therapeutic functions due to their diverse biological properties. This article focuses on the species Lucilia sericata and the benefits of larval therapy in patients who, due to hospitalization, have developed pressure ulcers and other difficult-to-heal wounds. Larval therapy, also known as maggot debridement therapy, employs sterile fly larvae to treat chronic, non-healing wounds by enzymatically degrading necrotic tissue and decreasing bacterial colonization. The larvae are applied to the wound for a period of 48–72 h, during which they effectively clean the wound and stimulate tissue regeneration. This therapeutic approach is particularly efficacious for recalcitrant wounds, such as diabetic foot ulcers and pressure sores, which have not responded to conventional treatments. Larvae may also constitute an alternative material in entomotoxicological studies to detect substances ingested at not only toxic but also therapeutic doses. The present work describes a method for assaying ciprofloxacin in L. sericata larvae using capillary electrophoresis coupled to mass spectrometry. The developed method features high sensitivity with a limit of quantification of 100 ± 0.018 ng/mL, as well as accuracy and precision estimated within 87%–103% and 1%–4%, respectively. An application of a simple and fast precipitation of proteins procedure for sample cleaning resulted in a highly satisfactory recovery of the analyte (90%–104%). The method was linear in a range of 100–1000 ng/mL with a determination coefficient higher than 0.9973. The method was used to determine ciprofloxacin in larval homogenate after antibiotic administration to the patient at a dose of 500 mg twice daily per os during application of the larvae dressing. Ciprofloxacin was shown to distribute from the patient's circulation to the larvae at a concentration of 150 ng/mL (750 ng/g).

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来源期刊
ELECTROPHORESIS
ELECTROPHORESIS 生物-分析化学
CiteScore
6.30
自引率
13.80%
发文量
244
审稿时长
1.9 months
期刊介绍: ELECTROPHORESIS is an international journal that publishes original manuscripts on all aspects of electrophoresis, and liquid phase separations (e.g., HPLC, micro- and nano-LC, UHPLC, micro- and nano-fluidics, liquid-phase micro-extractions, etc.). Topics include new or improved analytical and preparative methods, sample preparation, development of theory, and innovative applications of electrophoretic and liquid phase separations methods in the study of nucleic acids, proteins, carbohydrates natural products, pharmaceuticals, food analysis, environmental species and other compounds of importance to the life sciences. Papers in the areas of microfluidics and proteomics, which are not limited to electrophoresis-based methods, will also be accepted for publication. Contributions focused on hyphenated and omics techniques are also of interest. Proteomics is within the scope, if related to its fundamentals and new technical approaches. Proteomics applications are only considered in particular cases. Papers describing the application of standard electrophoretic methods will not be considered. Papers on nanoanalysis intended for publication in ELECTROPHORESIS should focus on one or more of the following topics: • Nanoscale electrokinetics and phenomena related to electric double layer and/or confinement in nano-sized geometry • Single cell and subcellular analysis • Nanosensors and ultrasensitive detection aspects (e.g., involving quantum dots, "nanoelectrodes" or nanospray MS) • Nanoscale/nanopore DNA sequencing (next generation sequencing) • Micro- and nanoscale sample preparation • Nanoparticles and cells analyses by dielectrophoresis • Separation-based analysis using nanoparticles, nanotubes and nanowires.
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