CEP肽-CRA2受体模块促进丛枝菌根共生。

IF 8.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Current Biology Pub Date : 2024-11-18 Epub Date: 2024-10-21 DOI:10.1016/j.cub.2024.09.058
Léa Pedinotti, Juliette Teyssendier de la Serve, Thibault Roudaire, Hélène San Clemente, Marielle Aguilar, Wouter Kohlen, Florian Frugier, Nicolas Frei Dit Frey
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引用次数: 0

摘要

C 端编码肽(CEPs)是一种小型分泌信号肽,可促进豆科植物的固氮根瘤共生,具体取决于土壤矿物氮的可用性。在Medicago truncatula中,它们的作用是由富含亮氨酸重复受体的类蛋白激酶COMPACT ROOT ARCHITECTURE 2(CRA2)介导的。2,3,4 与大多数陆生植物一样,在无机磷酸盐限制条件下,M. truncatula会与树根真菌建立另一种根内共生关系,即树根菌根共生(AMS)。5 我们在本研究中发现,在低磷酸盐条件下,CEP编码基因子集的表达得到增强,而低磷酸盐诱导的MtCEP1基因(之前已证明能促进固氮根瘤共生)的过表达能从真菌的最初进入点增强AMS。相反,介导 CEP 肽作用所需的 CRA2 受体功能缺失突变2 则会从相同的真菌最初进入阶段开始降低内生菌根的相互作用。转录组分析表明,cra2 突变体对关键磷酸盐转运和响应基因的调控以及建立 AMS 所需的股内酯激素的生物合成产生了负面影响。因此,Cra2 突变体根中的绞股蓝内酯含量急剧下降。总之,我们的研究表明,CEP/CRA2 途径能促进豆科植物的根瘤形成和 AMS,这取决于土壤中矿质养分的可用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The CEP peptide-CRA2 receptor module promotes arbuscular mycorrhizal symbiosis.

C-terminally encoded peptides (CEPs) are small secreted signaling peptides that promote nitrogen-fixing root nodulation symbiosis in legumes, depending on soil mineral nitrogen availability.1 In Medicago truncatula, their action is mediated by the leucine-rich repeat receptor-like protein kinase COMPACT ROOT ARCHITECTURE 2 (CRA2).2,3,4 Like most land plants, under inorganic phosphate limitation, M. truncatula establishes another root endosymbiotic interaction with arbuscular fungi, the arbuscular mycorrhizal symbiosis (AMS). Because this interaction is beneficial for the plant but has a high energetic cost, it is tightly controlled by host plants to limit fungal infections mainly depending on phosphate availability.5 We show in this study that the expression of a subset of CEP-encoding genes is enhanced in the low-phosphate conditions and that overexpression of the low-phosphate-induced MtCEP1 gene, previously shown to promote the nitrogen-fixing root nodulation symbiosis, enhances AMS from the initial entry point of the fungi. Conversely, a loss-of-function mutation of the CRA2 receptor required for mediating CEP peptide action2 decreases the endomycorrhizal interaction from the same initial fungal entry stage. Transcriptomic analyses revealed that the cra2 mutant is negatively affected in the regulation of key phosphate transport and response genes as well as in the biosynthesis of strigolactone hormones that are required for establishing AMS. Accordingly, strigolactone contents were drastically decreased in cra2 mutant roots. Overall, we showed that the CEP/CRA2 pathway promotes both root nodulation and AMS in legume plants, depending on soil mineral nutrient availability.

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来源期刊
Current Biology
Current Biology 生物-生化与分子生物学
CiteScore
11.80
自引率
2.20%
发文量
869
审稿时长
46 days
期刊介绍: Current Biology is a comprehensive journal that showcases original research in various disciplines of biology. It provides a platform for scientists to disseminate their groundbreaking findings and promotes interdisciplinary communication. The journal publishes articles of general interest, encompassing diverse fields of biology. Moreover, it offers accessible editorial pieces that are specifically designed to enlighten non-specialist readers.
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