Inasse Cherfi, Nasma Mahboub, Ikram Toumi, Salah Eddine Laouini, Gamil Gamal Hasan, Abderrhmane Bouafia, Fahad Alharthi, Talha Bin Emran
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引用次数: 0
摘要
多囊卵巢综合征(PCOS)以代谢和生殖功能障碍为特征,通常与血液中氧化应激指标升高有关。本研究探讨了青蒿叶提取物(青蒿 L)的潜在抗氧化特性、α-淀粉酶抑制活性及其对诱发多囊卵巢综合征大鼠的影响。对 10 只成熟的 Wistar 大鼠施用戊酸雌二醇诱导多囊卵巢综合征,对照组由 5 只成熟的 Wistar 大鼠组成。经过 16 天的诱导期后,大鼠被分为三组:对照组、多囊卵巢综合征组和连续 15 天口服 200 毫克/公斤体重的 A. campestris L. 提取物的实验组。采用 ELISA 技术测定血清中的卵泡刺激素(FSH)和黄体生成素(LH)水平。与多囊卵巢综合征组相比,接受 A. campestris L. 提取物治疗的组的 LH 水平明显降低。组织形态分析表明,卵泡数量和子宫颈层厚度发生了明显变化。这些研究结果表明,多囊卵巢综合症的症状明显减轻,这可能与 A. campestris L. 对氧化应激途径的影响有关。此外,A. campestris L.的水提取物对α-淀粉酶有很强的体外抑制作用,其 IC50 值为 2.418 µg/mL。
Assessment of Artemisia campestris L. Leaf Extract Effects on Polycystic Ovarian Syndrome in Rats, Antioxidant and α-Amylase Inhibition Activities.
Polycystic Ovarian Syndrome (PCOS) is characterized by metabolic and reproductive dysfunction, often associated with elevated oxidative stress markers in the bloodstream. This study examines the potential antioxidant properties and α-amylase inhibitory activity of Artemisia campestris leaves extract (Artemisia campestris L) and its effects on rats with induced PCOS. Estradiol valerate was administered to ten mature Wistar rats to induce PCOS, while a control group consisted of five mature Wistar rats. Following a 16-day induction period, the rats were categorized into three groups: a control group, a PCOS group, and an experimental group receiving 200 mg/kg body weight of A. campestris L. extract orally for 15 days. Serum levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) were measured using the ELISA technique. The group treated with A. campestris L. extract exhibited significantly reduced LH levels compared to the PCOS group. Histomorphometric analysis indicated notable changes in follicle counts and the thickness of the theca layer. These findings suggest a significant alleviation of PCOS symptoms, potentially linked to the effects of A. campestris L. on oxidative stress pathways. Furthermore, aqueous extracts of A. campestris L. displayed potent in vitro inhibition of α-amylase, with an IC50 value of 2.418 µg/mL.
期刊介绍:
Chemistry & Biodiversity serves as a high-quality publishing forum covering a wide range of biorelevant topics for a truly international audience. This journal publishes both field-specific and interdisciplinary contributions on all aspects of biologically relevant chemistry research in the form of full-length original papers, short communications, invited reviews, and commentaries. It covers all research fields straddling the border between the chemical and biological sciences, with the ultimate goal of broadening our understanding of how nature works at a molecular level.
Since 2017, Chemistry & Biodiversity is published in an online-only format.