细胞毒性 CD8+ αβ T 细胞和 γδ T 细胞胞外囊泡的比较分析

IF 5.1 2区 生物学 Q2 CELL BIOLOGY
Cells Pub Date : 2024-10-21 DOI:10.3390/cells13201745
Lisa Griesel, Patrick Kaleja, Andreas Tholey, Marcus Lettau, Ottmar Janssen
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引用次数: 0

摘要

背景:细胞毒性 CD8+αβ T 细胞和 γδ T 细胞虽然属于免疫系统的不同分支,但它们利用共同的细胞溶解效应物,包括 FasL、颗粒酶、穿孔素和颗粒溶素。效应蛋白储存在溶酶体相关效应囊(LREVs)的不同亚群中,遇到靶细胞时释放到免疫突触。值得注意的是,在活化细胞中,溶酶体相关效应囊泡和其他可能的囊泡不断产生并以细胞外囊泡(EVs)的形式释放出来。据推测,EVs 可作为本地微环境或远处细胞间通信的媒介:方法:用差速和超速离心法从培养上清液中富集活化和扩增的细胞毒性 CD8+ αβ T 细胞或 γδ T 细胞的 EVs,并用纳米颗粒追踪分析和 Western 印迹法对其进行表征。为了进行比较蛋白质组学分析,对来自两种细胞类型的EV制备物进行了串联质量标签(TMT10plex)等位标记,并进行了质谱分析:结果:在细胞毒性 CD8+ αβ T 细胞和 γδ T 细胞的 EV 制剂中定量检测了 686 种蛋白质。这两种细胞群共享一组主要的相似丰度蛋白,而在 CD8+ αβ T 细胞或 γδ T 细胞中出现较高丰度水平的蛋白要少得多。据我们所知,我们首次对来自细胞毒性 CD8+ αβ T 细胞和 γδ T 细胞的 EVs 进行了比较分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative Analysis of Extracellular Vesicles from Cytotoxic CD8+ αβ T Cells and γδ T Cells.

Background: Although belonging to different branches of the immune system, cytotoxic CD8+ αβ T cells and γδ T cells utilize common cytolytic effectors including FasL, granzymes, perforin and granulysin. The effector proteins are stored in different subsets of lysosome-related effector vesicles (LREVs) and released to the immunological synapse upon target cell encounter. Notably, in activated cells, LREVs and potentially other vesicles are continuously produced and released as extracellular vesicles (EVs). Presumably, EVs serve as mediators of intercellular communication in the local microenvironment or at distant sites.

Methods: EVs of activated and expanded cytotoxic CD8+ αβ T cells or γδ T cells were enriched from culture supernatants by differential and ultracentrifugation and characterized by nanoparticle tracking analyses and Western blotting. For a comparative proteomic profiling, EV preparations from both cell types were isobaric labeled with tandem mass tags (TMT10plex) and subjected to mass spectrometry analysis.

Results: 686 proteins were quantified in EV preparations of cytotoxic CD8+ αβ T cells and γδ T cells. Both populations shared a major set of similarly abundant proteins, while much fewer proteins presented higher abundance levels in either CD8+ αβ T cells or γδ T cells. To our knowledge, we provide the first comparative analysis of EVs from cytotoxic CD8+ αβ T cells and γδ T cells.

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来源期刊
Cells
Cells Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
9.90
自引率
5.00%
发文量
3472
审稿时长
16 days
期刊介绍: Cells (ISSN 2073-4409) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to cell biology, molecular biology and biophysics. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided.
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