对进行三系分化的间充质干细胞中的胶原、NAD(P)H 和黄素自发荧光进行多光谱成像。

IF 5.1 2区 生物学 Q2 CELL BIOLOGY
Cells Pub Date : 2024-10-18 DOI:10.3390/cells13201731
Jared M Campbell, Saabah B Mahbub, Ayad G Anwer, Abbas Habibalahi, Stan Gronthos, Sharon Paton, Shane T Grey, Lindsay E Wu, Robert B Gilchrist, Ewa M Goldys
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引用次数: 0

摘要

了解分化的分子机制对再生医学和发育生物学非常重要。本研究旨在描述糖酵解/氧化磷酸化平衡作为间充质干细胞(MSC)分化驱动力的作用。细胞在正常条件下维持21天,或在刺激下向间充质干细胞三系细胞类型分化。细胞自发荧光的多光谱成像是一种非侵入性方法,可对原位培养物进行连续成像。胶原蛋白、NAD(P)H 和黄素的光谱信号没有混合。与对照组相比,在软骨生成条件下培养的间充质干细胞表现出胶原蛋白水平的增加。在诱导成骨过程中,间充质干细胞在培养早期阶段的胶原蛋白水平相对于对照组有所增加;然而,对照组细胞在汇合过程中胶原蛋白水平也有所增加。在成脂条件下培养的间充质干细胞的胶原蛋白水平低于对照组。在软骨生成过程中,氧化还原比率(RR;NAD(P)H/黄素)立即下降,与对照组细胞相比,这种早期效应在整个培养过程中持续存在,而对照组细胞的氧化还原比率似乎增加了,这与成骨过程类似。与对照细胞相比,脂肪生成在第2天导致RR小幅增加,随后持续下降。软骨和脂肪分化有利于氧化磷酸化,而成骨和间充质干细胞过度生长则导致糖酵解代谢。在对这些发现以及文献中的各种报道进行考虑后,得出的结论是,氧化磷酸化和糖酵解的增强都不是典型分化模式的基本要素,研究人员应避免将转变解释为分化的标志。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Multispectral Imaging of Collagen, NAD(P)H and Flavin Autofluorescence in Mesenchymal Stem Cells Undergoing Trilineage Differentiation.

Understanding the molecular mechanisms of differentiation is important for regenerative medicine and developmental biology. This study aims to characterise the role of the glycolysis/oxidative phosphorylation balance as a driver of mesenchymal stem cell (MSC) differentiation. Cells were maintained in normal conditions or stimulated towards the MSC trilineage cell types over 21 days. Multispectral imaging of cell autofluorescence was applied as a non-invasive methodology to continuously image cultures in situ. Spectral signals for collagen, NAD(P)H, and flavins were unmixed. MSCs cultured under chondrogenic conditions exhibited increased collagen levels relative to controls. Following osteogenic induction, MSCs showed increased collagen levels relative to controls during the earlier stages of culture; however, control cells increased their collagen levels as they became confluent. MSCs cultured under adipogenic conditions exhibited lower levels of collagen than controls. The redox ratio (RR; NAD(P)H/flavins) immediately decreased during chondrogenesis, with this early effect persisting throughout the culture compared to control cells, which appeared to increase their RR, similar to osteogenesis. Adipogenesis resulted in a small increase in RR on day 2 relative to control cells, followed by a persistent decrease. Chondrogenic and adipogenic differentiation favoured oxidative phosphorylation, whereas osteogenesis and MSC overgrowth resulted in a glycolytic metabolism. Following consideration of these findings, as well as the diverse reports in the literature, it is concluded that neither enhanced oxidative phosphorylation nor glycolysis are fundamental to the canonical modes of differentiation, and researchers should avoid interpreting shifts as indicating differentiation.

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来源期刊
Cells
Cells Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
9.90
自引率
5.00%
发文量
3472
审稿时长
16 days
期刊介绍: Cells (ISSN 2073-4409) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to cell biology, molecular biology and biophysics. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided.
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