对多个肠道组织进行共识基因共表达分析,以确定与肉鸡腹部脂肪沉积相关的关键基因和通路。

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
S Wang, Y Liu, S Liu, Z Qin, J Lu, R Zhang, H Yuan
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引用次数: 0

摘要

1.腹部脂肪沉积(AFD)受多种肠道组织的调控,肠道组织功能的变化与 AFD 有关。目前,整合多个肠道组织的转录组数据以探讨肉鸡过度腹脂沉积的研究还鲜有报道。 2. 在本研究中,我们利用公开的转录组数据集构建了高腹脂和低腹脂肉鸡品系(HL 和 LL)十二指肠、空肠、回肠和盲肠的共识基因共表达网络。结合功能富集分析和差异基因表达分析的结果,研究了可能影响 AFD.3 的四个肠道组织的基因和生物通路。在一个表达模块中,NDUFA5、NDUFS6、NDUFA4、NDUFS4、ATP5H、ATP5J 和 ATP5C1 在氧化磷酸化途径中显著富集,GPX2 和 GSR 在谷胱甘肽代谢途径中显著富集。在其他模块(ALDH7A1、ACSBG1、THEM4 和 DECR1)中发现了脂质代谢相关基因,这些基因可能通过调节脂质代谢与 AFD 有关。有趣的是,在第一个模块中,有 12 个基因在蛋白酶体通路中明显富集,并且在 HL 禽的四个肠道组织中与 LL 禽相比明显下调,这表明蛋白酶体与 AFD 之间存在联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Consensus gene co-expression analysis across multiple intestinal tissues to identify key genes and pathways associated with abdominal fat deposition in broilers.

1. Abdominal fat deposition (AFD) is regulated by multiple intestinal tissues, and changes in the function of intestinal tissues are associated with AFD. Currently, integration of transcriptomic data across multiple intestinal tissues to explore excessive AFD has rarely been reported in broilers.2. In this study, a consensus gene co-expression network across the duodenum, jejunum, ileum and caecum of high- and low-abdominal fat broiler lines (HL and LL) was constructed using a publicly available transcriptomic data set. Combining the results of functional enrichment analyses and differential gene expression analyses, this investigated the genes and biological pathways across the four intestinal tissues that might influence AFD.3. In one expression module, NDUFA5, NDUFS6, NDUFA4, NDUFS4, ATP5H, ATP5J and ATP5C1 were significantly enriched in the oxidative phosphorylation pathway, with GPX2 and GSR significantly enriched in the glutathione metabolism pathway. These genes were significantly downregulated in the four intestinal tissues of the HL compared to LL chickens, which may be associated with AFD by increasing intestinal permeability.4. Lipid metabolism relevant genes were identified in other modules (ALDH7A1, ACSBG1, THEM4 and DECR1), which may be linked to AFD through regulation of lipid metabolism. Interestingly, in the first module, 12 genes were significantly enriched in the proteasome pathway and significantly downregulated in the four intestinal tissues in HL birds compared to LL birds, indicating a link between the proteasome and AFD.

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来源期刊
British Poultry Science
British Poultry Science 农林科学-奶制品与动物科学
CiteScore
3.90
自引率
5.00%
发文量
88
审稿时长
4.5 months
期刊介绍: From its first volume in 1960, British Poultry Science has been a leading international journal for poultry scientists and advisers to the poultry industry throughout the world. Over 60% of the independently refereed papers published originate outside the UK. Most typically they report the results of biological studies with an experimental approach which either make an original contribution to fundamental science or are of obvious application to the industry. Subjects which are covered include: anatomy, embryology, biochemistry, biophysics, physiology, reproduction and genetics, behaviour, microbiology, endocrinology, nutrition, environmental science, food science, feeding stuffs and feeding, management and housing welfare, breeding, hatching, poultry meat and egg yields and quality.Papers that adopt a modelling approach or describe the scientific background to new equipment or apparatus directly relevant to the industry are also published. The journal also features rapid publication of Short Communications. Summaries of papers presented at the Spring Meeting of the UK Branch of the WPSA are published in British Poultry Abstracts .
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