保加利亚奶酪和葱酸奶中的益生菌株对溶血性葡萄球菌的抗菌潜力

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioMed Research International Pub Date : 2024-10-15 eCollection Date: 2024-01-01 DOI:10.1155/2024/2978083
Pardis Amigh, Yasaman Ahmadi, Milad Mohkam, Dariush Shokri
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引用次数: 0

摘要

由于抗生素耐药性,医院感染的发病率不断上升,因此有必要寻找替代治疗药物,如益生菌。本研究旨在分离和评估益生菌对泌尿道感染(UTI)的主要病原体--肥厚葡萄球菌的疗效。共从临床样本中分离出 100 株沙弗氏葡萄球菌,并通过盘扩散法进行抗生素药敏试验。同时,从保加利亚奶酪和香葱酸奶中分离出益生菌,并评估了它们对沙普氏菌株的抗菌活性。采用微孔板检测法评估益生菌上清液的抑菌潜力,以 1/2 稀释度测定最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。细胞毒性采用 MTT 法进行评估,高效液相色谱法(HPLC)用于分析益生菌产生的有机酸浓度。结果表明,所有无患子菌株都对四环素和强力霉素有抗药性,但对其他抗生素易感。鼠李糖乳杆菌菌株 M 和 B 对沙普氏菌分离株具有显著的抗菌和抗生物膜活性。这些益生菌对大多数抗生素都有敏感性,而且缺乏毒力因子,这表明它们可以安全地用于治疗。益生菌产生的有机酸被鉴定为乳酸、乙酸和甲酸。总之,鼠李糖 M 株和 B 株对溶血性沙雷氏菌有很强的抗菌作用,表明它们具有治疗UTI 的潜力。为了验证这些发现并探索这些益生菌在临床应用中的可能性,还需要进一步的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antimicrobial Potential of Probiotic Strains From Bulgarian Cheese and Shallot Yogurt Against Staphylococcus saprophyticus.

The escalating incidence of hospital infections due to antibiotic resistance necessitates the identification of alternative therapeutic agents such as probiotics. This study was designed to isolate and evaluate the efficacy of probiotics against Staphylococcus saprophyticus, a prevalent etiological agent of urinary tract infections (UTIs). A total of 100 S. saprophyticus strains were isolated from clinical samples and subjected to antibiotic susceptibility testing via the disc diffusion method. Concurrently, probiotic bacteria were isolated from Bulgarian cheese and shallot yogurt, and their antibacterial activity against S. saprophyticus strains was assessed. The inhibitory potential of probiotic supernatants was evaluated using microtiter plate assays, with the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) determined at a 1/2 dilution. Cytotoxicity was evaluated using the MTT assay, and high-performance liquid chromatography (HPLC) was employed to analyze the concentrations of organic acids produced by the probiotics. The results revealed that all S. saprophyticus strains were resistant to tetracycline and doxycycline but susceptible to other antibiotics. Lactobacillus rhamnosus strains M and B demonstrated notable antibacterial and antibiofilm activity against S. saprophyticus isolates. These probiotics exhibited susceptibility to most antibiotics and lacked virulence factors, suggesting their safety for therapeutic use. The organic acids produced by the probiotics were identified as lactic acid, acetic acid, and formic acid. In conclusion, L. rhamnosus strains M and B exhibit potent antimicrobial properties against S. saprophyticus, indicating their potential as therapeutic agents for UTIs. Further research is warranted to validate these findings and explore the possibility of these probiotics in clinical applications.

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来源期刊
BioMed Research International
BioMed Research International BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL
CiteScore
6.70
自引率
0.00%
发文量
1942
审稿时长
19 weeks
期刊介绍: BioMed Research International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies covering a wide range of subjects in life sciences and medicine. The journal is divided into 55 subject areas.
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