人类和哥廷根小型猪乳腺上皮细胞生物能代谢比较研究

IF 2.8 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. General subjects Pub Date : 2024-10-20 DOI:10.1016/j.bbagen.2024.130728

Cristina Algieri , Chiara Bernardini , Debora La Mantia , Fabiana Trombetti , Monica Forni , Salvatore Nesci

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引用次数: 0

摘要

对人类（h）和哥廷根小型猪（mp）的乳腺上皮细胞（MECs）进行了分析，以比较它们通过氧化磷酸化和糖酵解产生 ATP 的能力。在基础和应激情况下产生的 ATP 突出显示了两种原代细胞系相同的代谢潜力。然而，从数量上看，hMECs 的 ATP 生成率高于 mpMECs。相反，mpMECs 的氧化细胞呼吸利用了最大呼吸能力，以支持可能需要细胞代谢过度努力的病理生理情况或应激条件。由于 mpMECs 主要利用与 hMECs 相似的氧化新陈代谢，因此我们通过新陈代谢表征确认 mpMECs 是转化医学方法中一种潜在的替代细胞模型。

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A comparative study of bioenergetic metabolism on mammary epithelial cells from humans and Göttingen Minipigs

Mammary epithelial cells (MECs) of humans (h) and Göttingen Minipigs (mp) were analyzed to compare their ability to perform ATP production by oxidative phosphorylation and glycolysis. The ATP production under basal and stressor situations highlights the same metabolic potential of both primary cell lines. However, quantitively the ATP production rate of hMECs was higher than mpMECs. Conversely, oxidative cell respiration in mpMECs exploits a maximum respiratory capacity to support pathophysiological circumstances or stress conditions that could require an excessive effort of cell metabolism. Since mpMECs primarily utilize an oxidative metabolism similar to hMECs, the metabolic characterization conducted allows us to confirm that mpMECs represent a potential alternative cellular model in the translational medicine approach.

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来源期刊

Biochimica et biophysica acta. General subjects 生物-生化与分子生物学

CiteScore

6.40

自引率

0.00%

发文量

139

审稿时长

30 days

期刊介绍： BBA General Subjects accepts for submission either original, hypothesis-driven studies or reviews covering subjects in biochemistry and biophysics that are considered to have general interest for a wide audience. Manuscripts with interdisciplinary approaches are especially encouraged.