槲皮素-β-环糊精复合物介导的光动力对大肠杆菌 O157:H7 的抗菌作用及其机制

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Tao Liu, Yuzhang Zhu, Jiahui Wang, Xiangyu Hong, Mi Liu, Chaonan Kong, Rui Zhou, Xianke Li, Lifang Yang
{"title":"槲皮素-β-环糊精复合物介导的光动力对大肠杆菌 O157:H7 的抗菌作用及其机制","authors":"Tao Liu,&nbsp;Yuzhang Zhu,&nbsp;Jiahui Wang,&nbsp;Xiangyu Hong,&nbsp;Mi Liu,&nbsp;Chaonan Kong,&nbsp;Rui Zhou,&nbsp;Xianke Li,&nbsp;Lifang Yang","doi":"10.1007/s00203-024-04175-1","DOIUrl":null,"url":null,"abstract":"<div><p>Quercetin is a natural flavonoid with antioxidant, anti-inflammatory, and antibacterial properties. This work aimed to formulate quercetin-cyclodextrin microcapsules (QT-β-CD) while examining their photodynamic antibacterial effects and underlying mechanisms in detail. Characterization of the QT-β-CD was conducted using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The bacteriostatic effects of UV-A irradiation on <i>Escherichia coli</i> O157:H7 (<i>E. coli</i> O157:H7) were investigated. The photodynamic impact of QT-β-CD was assessed by analyzing hydrogen peroxide (H₂O₂) production. The antimicrobial activity was further elucidated through examinations of cell membrane integrity, protein damage, changes in cellular motility, biofilm formation, and extracellular polysaccharide reduction. The effect of QT-β-CD on <i>LuxS</i> and <i>motA</i> gene expression in <i>E. coli</i> O157:H7 was investigated by RT-qPCR. The findings demonstrated that QT-β-CD exhibited potent photodynamic properties and functioned as an efficient photosensitizer, causing substantial damage to <i>E. coli</i> O157:H7 cells. These results underscore the potential of quercetin as an antimicrobial agent for food preservation.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3000,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antibacterial effects and mechanisms of quercetin-β-cyclodextrin complex mediated photodynamic on Escherichia coli O157:H7\",\"authors\":\"Tao Liu,&nbsp;Yuzhang Zhu,&nbsp;Jiahui Wang,&nbsp;Xiangyu Hong,&nbsp;Mi Liu,&nbsp;Chaonan Kong,&nbsp;Rui Zhou,&nbsp;Xianke Li,&nbsp;Lifang Yang\",\"doi\":\"10.1007/s00203-024-04175-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Quercetin is a natural flavonoid with antioxidant, anti-inflammatory, and antibacterial properties. This work aimed to formulate quercetin-cyclodextrin microcapsules (QT-β-CD) while examining their photodynamic antibacterial effects and underlying mechanisms in detail. Characterization of the QT-β-CD was conducted using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The bacteriostatic effects of UV-A irradiation on <i>Escherichia coli</i> O157:H7 (<i>E. coli</i> O157:H7) were investigated. The photodynamic impact of QT-β-CD was assessed by analyzing hydrogen peroxide (H₂O₂) production. The antimicrobial activity was further elucidated through examinations of cell membrane integrity, protein damage, changes in cellular motility, biofilm formation, and extracellular polysaccharide reduction. The effect of QT-β-CD on <i>LuxS</i> and <i>motA</i> gene expression in <i>E. coli</i> O157:H7 was investigated by RT-qPCR. The findings demonstrated that QT-β-CD exhibited potent photodynamic properties and functioned as an efficient photosensitizer, causing substantial damage to <i>E. coli</i> O157:H7 cells. These results underscore the potential of quercetin as an antimicrobial agent for food preservation.</p></div>\",\"PeriodicalId\":8279,\"journal\":{\"name\":\"Archives of Microbiology\",\"volume\":\"206 11\",\"pages\":\"\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2024-10-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archives of Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00203-024-04175-1\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s00203-024-04175-1","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

槲皮素是一种天然类黄酮,具有抗氧化、抗炎和抗菌特性。本研究旨在配制槲皮素-环糊精微胶囊(QT-β-CD),同时详细研究其光动力抗菌效果及其内在机制。利用傅立叶变换红外光谱(FTIR)、X射线衍射(XRD)和热重分析(TGA)对QT-β-CD进行了表征。研究了紫外线-A 照射对大肠杆菌 O157:H7 (E. coli O157:H7)的抑菌作用。通过分析过氧化氢(H₂O₂)的产生,评估了 QT-β-CD 的光动力影响。通过检测细胞膜完整性、蛋白质损伤、细胞运动变化、生物膜形成和细胞外多糖减少,进一步阐明了QT-β-CD的抗菌活性。通过 RT-qPCR 研究了 QT-β-CD 对大肠杆菌 O157:H7 中 LuxS 和 motA 基因表达的影响。研究结果表明,QT-β-CD 具有强大的光动力特性,是一种高效的光敏剂,可对大肠杆菌 O157:H7 细胞造成严重破坏。这些结果凸显了槲皮素作为食品保鲜抗菌剂的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antibacterial effects and mechanisms of quercetin-β-cyclodextrin complex mediated photodynamic on Escherichia coli O157:H7

Quercetin is a natural flavonoid with antioxidant, anti-inflammatory, and antibacterial properties. This work aimed to formulate quercetin-cyclodextrin microcapsules (QT-β-CD) while examining their photodynamic antibacterial effects and underlying mechanisms in detail. Characterization of the QT-β-CD was conducted using Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and thermogravimetric analysis (TGA). The bacteriostatic effects of UV-A irradiation on Escherichia coli O157:H7 (E. coli O157:H7) were investigated. The photodynamic impact of QT-β-CD was assessed by analyzing hydrogen peroxide (H₂O₂) production. The antimicrobial activity was further elucidated through examinations of cell membrane integrity, protein damage, changes in cellular motility, biofilm formation, and extracellular polysaccharide reduction. The effect of QT-β-CD on LuxS and motA gene expression in E. coli O157:H7 was investigated by RT-qPCR. The findings demonstrated that QT-β-CD exhibited potent photodynamic properties and functioned as an efficient photosensitizer, causing substantial damage to E. coli O157:H7 cells. These results underscore the potential of quercetin as an antimicrobial agent for food preservation.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Archives of Microbiology
Archives of Microbiology 生物-微生物学
CiteScore
4.90
自引率
3.60%
发文量
601
审稿时长
3 months
期刊介绍: Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信