通过单细胞和大量 RNA 测序综合分析牙周炎中杯突症的作用。

IF 3 4区医学 Q1 MEDICINE, GENERAL & INTERNAL

Archives of Medical Science Pub Date : 2024-08-29 eCollection Date: 2024-01-01 DOI:10.5114/aoms/192414

Yuanyuan Fu, Chongbin Zhong, Junhe Cui, Shengjie Xie, Chuang Guo

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引用次数: 0

摘要

导言：微量矿物质对牙周健康至关重要，而微量矿物质水平失衡与慢性牙周炎有关，其中包括铜。我们试图探究铜中毒与牙周炎之间的关系：方法：利用大量 RNA-seq 和单细胞 RNA 测序数据分析杯突相关基因（CRGs）在牙周炎中的表达。建立了小鼠牙周炎模型以验证关键基因的表达：结果：杯突相关基因在牙周炎中的表达存在差异。RT-PCR结果证实了小鼠模型中五个关键基因的改变：结论：杯突在牙周炎的病理生理过程中起着重要作用。

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A comprehensive analysis of the role of cuproptosis in periodontitis through integrated analysis of single-cell and bulk RNA sequencing.

Introduction: Trace mineral micronutrients are essential for periodontal health, and unbalanced levels of trace minerals are related to chronic periodontitis, including copper. We attempted to explore the relationship between cuproptosis and periodontitis.

Methods: Bulk RNA-seq and single-cell RNA sequencing data were used to analyze expression of cuproptosis-related genes (CRGs) in periodontitis. A mouse periodontitis model was established to verify the expression of key genes.

Results: Cuproptosis-related genes were differentially expressed in periodontitis. RT-PCR results confirmed the alteration of five key genes in the mouse model.

Conclusions: Cuproptosis is an important player in the pathophysiological process of periodontitis.

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来源期刊

Archives of Medical Science 医学-医学：内科

CiteScore

4.90

自引率

7.90%

发文量

139

审稿时长

1.7 months

期刊介绍： Archives of Medical Science (AMS) publishes high quality original articles and reviews of recognized scientists that deal with all scientific medicine. AMS opens the possibilities for young, capable scientists. The journal would like to give them a chance to have a publication following matter-of-fact, professional review by outstanding, famous medical scientists. Thanks to that they will have an opportunity to present their study results and/or receive useful advice about the mistakes they have made so far. The second equally important aim is a presentation of review manuscripts of recognized scientists about the educational capacity, in order that young scientists, often at the beginning of their scientific carrier, could constantly deepen their medical knowledge and be up-to-date with current guidelines and trends in world-wide medicine. The fact that our educational articles are written by world-famous scientists determines their innovation and the highest quality.