Daniela Kallíope de Sá Paraskevopoulos, Carlos Henrique Camargo, Priscila Koba Kodato, Amanda Yaeko Yamada, Adriana Aparecida Buzzo Almodovar, Ellen Gameiro Hilinski, Alexandre Inácio de Paula, Elaine Fernanda Irineu, Sandra Rodrigues Barrio, Cibele L Fonseca, Cristiano de Melo Gamba, Nair Hosino, Augusto Yamaguti, João S de Mendonça, Thaís Guimarães
{"title":"重症监护室爆发的伯克霍尔德氏菌污染与受污染的浴液有关：控制和微生物学发现。","authors":"Daniela Kallíope de Sá Paraskevopoulos, Carlos Henrique Camargo, Priscila Koba Kodato, Amanda Yaeko Yamada, Adriana Aparecida Buzzo Almodovar, Ellen Gameiro Hilinski, Alexandre Inácio de Paula, Elaine Fernanda Irineu, Sandra Rodrigues Barrio, Cibele L Fonseca, Cristiano de Melo Gamba, Nair Hosino, Augusto Yamaguti, João S de Mendonça, Thaís Guimarães","doi":"10.1016/j.ajic.2024.10.006","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>The Burkholderia cepacia complex comprises a group of bacteria with a growing threat as a contaminant of nonsterile solutions. We describe an outbreak of a Burkholderia cepacia complex involving patients at intensive care unit related to a no-rinse bathing solution (NRBS).</p><p><strong>Methods: </strong>We carried out patients, environmental and laboratory investigation performing analyses of cases, pulsed-field-gel electrophoresis and whole genome sequence (WGS) of isolates.</p><p><strong>Results: </strong>We investigated 32 cases and 16 isolates that were identified as Burkholderia contaminans, belonging to 2 different clones. One clone (new ST2175) was identified in 6 sequences (4 from patients and 2 from bath cart samples) and for the remaining 10 isolates (7 isolates from patients and 3 from NRBS) we identified the ST762. The investigation demonstrated that NRBS was the source of the outbreak caused by ST762 clone of B contaminans.</p><p><strong>Discussion: </strong>Early suspicion of a common source, rapid implementation of control measures, and laboratory support are vital in an outbreak investigation. We also highlight the role of WGS which was very important to conclude our investigation regarding environmental samples and bacterial typing.</p><p><strong>Conclusions: </strong>We highlight the need to regulate water-based products and the role of WGS for investigate environmental samples.</p>","PeriodicalId":7621,"journal":{"name":"American journal of infection control","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Burkholderia contaminans outbreak in an intensive care unit associated with contaminated bath solution: Control and microbiological findings.\",\"authors\":\"Daniela Kallíope de Sá Paraskevopoulos, Carlos Henrique Camargo, Priscila Koba Kodato, Amanda Yaeko Yamada, Adriana Aparecida Buzzo Almodovar, Ellen Gameiro Hilinski, Alexandre Inácio de Paula, Elaine Fernanda Irineu, Sandra Rodrigues Barrio, Cibele L Fonseca, Cristiano de Melo Gamba, Nair Hosino, Augusto Yamaguti, João S de Mendonça, Thaís Guimarães\",\"doi\":\"10.1016/j.ajic.2024.10.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>The Burkholderia cepacia complex comprises a group of bacteria with a growing threat as a contaminant of nonsterile solutions. 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The investigation demonstrated that NRBS was the source of the outbreak caused by ST762 clone of B contaminans.</p><p><strong>Discussion: </strong>Early suspicion of a common source, rapid implementation of control measures, and laboratory support are vital in an outbreak investigation. We also highlight the role of WGS which was very important to conclude our investigation regarding environmental samples and bacterial typing.</p><p><strong>Conclusions: </strong>We highlight the need to regulate water-based products and the role of WGS for investigate environmental samples.</p>\",\"PeriodicalId\":7621,\"journal\":{\"name\":\"American journal of infection control\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-10-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"American journal of infection control\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.ajic.2024.10.006\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"American journal of infection control","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.ajic.2024.10.006","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
A Burkholderia contaminans outbreak in an intensive care unit associated with contaminated bath solution: Control and microbiological findings.
Background: The Burkholderia cepacia complex comprises a group of bacteria with a growing threat as a contaminant of nonsterile solutions. We describe an outbreak of a Burkholderia cepacia complex involving patients at intensive care unit related to a no-rinse bathing solution (NRBS).
Methods: We carried out patients, environmental and laboratory investigation performing analyses of cases, pulsed-field-gel electrophoresis and whole genome sequence (WGS) of isolates.
Results: We investigated 32 cases and 16 isolates that were identified as Burkholderia contaminans, belonging to 2 different clones. One clone (new ST2175) was identified in 6 sequences (4 from patients and 2 from bath cart samples) and for the remaining 10 isolates (7 isolates from patients and 3 from NRBS) we identified the ST762. The investigation demonstrated that NRBS was the source of the outbreak caused by ST762 clone of B contaminans.
Discussion: Early suspicion of a common source, rapid implementation of control measures, and laboratory support are vital in an outbreak investigation. We also highlight the role of WGS which was very important to conclude our investigation regarding environmental samples and bacterial typing.
Conclusions: We highlight the need to regulate water-based products and the role of WGS for investigate environmental samples.
期刊介绍：
AJIC covers key topics and issues in infection control and epidemiology. Infection control professionals, including physicians, nurses, and epidemiologists, rely on AJIC for peer-reviewed articles covering clinical topics as well as original research. As the official publication of the Association for Professionals in Infection Control and Epidemiology (APIC)
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