细菌联合体通过暗发酵从共底物中生产生物氢。

IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2024-11-01 Epub Date: 2024-10-25 DOI:10.1007/s13205-024-04106-3
Chelladurai Mumtha, Pambayan Ulagan Mahalingam
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引用次数: 0

摘要

氢是一种清洁能源载体,可用作燃料电池的燃料。需要探索利用其他废弃生物质进行暗发酵生物制氢,作为未来可持续生物制氢的替代方法。本研究采用实验室规模的生物反应器,利用细菌群在 37 ℃ 下从共基质中生产生物氢。在实验设置中,使用了一个工作容积为 1 升的反应器,通过细菌单培养基和联合培养基生产生物氢。批量实验在 37 °C、初始 pH 值为 7.0、乳清和甘蔗渣的混合比例为 600:300 的条件下进行。在暗发酵过程中测定了共底物的总固体(TS)、挥发性固体(VS)、总化学需氧量(TCOD)、可溶性化学需氧量(SCOD)和产氢率(HPR)。利用扫描电镜成像技术测定了发酵过程后结合在共底物上的生物产氢菌的形态变化。当细菌附着在基质上时,会导致基质降解,形成孔洞并使表面积开裂。观察了细菌群的生物制氢水平,结果显示制氢率为 35.9 mL H2/L/h。在发酵产氢方面,它与之前研究的大多数产氢细菌(尤其是细菌联合体)的产氢率十分相似,这表明要找到一种优秀的发酵产氢细菌菌株可能非常困难,甚至不可能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Biohydrogen production from co-substrates through dark fermentation by bacterial consortium.

Hydrogen is a clean energy carrier that can be used as fuel for fuel cells. Dark fermentative biohydrogen production with other waste biomass needs to be explored as an alternative for sustainable biohydrogen production in future. In this study, lab-scale bioreactor were carried out to produce biohydrogen from co-substrates using bacterial consortium at 37 ℃. For the experimental setup, a 1-L-working-volume reactor was used for biohydrogen production by bacterial monocultures and consortium on co-substrates. A batch experiment was performed at 37 °C with an initial pH of 7.0 and a mixing ratio of 600:300 between dairy whey and sugarcane bagasse. Total solids (TS), volatile solids (VS), total chemical oxygen demand (TCOD), soluble chemical oxygen demand (SCOD), and hydrogen production rate (HPR) were determined from co-substrates during the dark fermentation process. Morphologic changes of biohydrogen producing bacteria binds on co-substrates after the fermentation process were determined using SEM imaging. The bacteria can degrade the substrate when they attach to it causing hole formation and cracked the surface area. The level of biohydrogen production by bacterial consortium was observed and the results revealed a hydrogen production rate of 35.9 mL H2/L/h. In fermentative H2 production, it is quite similar to that of most H2-producing bacteria previously studied, especially that of the bacterial consortium, and this indicates that the attempt to find an outstanding bacterial strain for fermentative H2 production might be very difficult if not impossible.

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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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