使用涂层刀片喷雾质谱法快速鉴定牛肾中的抗生素残留。

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Analytical and Bioanalytical Chemistry Pub Date : 2024-12-01 Epub Date: 2024-10-22 DOI:10.1007/s00216-024-05605-1
Josha Jager, Sjors Rasker, Ane Arrizabalaga-Larrañaga, Rita Boerrigter-Eenling, Michel Rapallini, Marco Blokland
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引用次数: 0

摘要

根据第 2017/625 号法规(欧盟),欧洲允许在食用动物中使用某些抗生素。然而,使用可能会导致动物源性食品中出现抗生素残留。最高残留限量(MRL)的制定是为了保护消费者。出于监测目的,对动物基质进行检测,以验证其是否符合这些最高残留限量。首先要对(屠宰的)食用动物基质进行筛查,通常采用微生物检测法。对于控制实验室来说,更快地进行抗生素筛选测试将是一个优势。因此,本研究首次介绍了使用涂层刀片喷雾(CBS)和直接质谱(MS)分析筛查完整牛肾脏中的四环素类、磺胺类、喹诺酮类和大环内酯类药物残留。采用优化的工作流程,每个叶片使用两种不同的解吸/电离溶液,每个样品可在 1 分钟内筛选出目标化合物。CBS-MS 方法的原理验证符合 (EU) 2021/808 标准,43 种分析物的 CCβ 筛选值为 0.1 × 最大残留限量,4 种分析物的 CCβ 筛选值为 0.5 × 最大残留限量,禁用物质达索酮的 CCβ 筛选值为 2.5 µg kg-1。所开发的方法成功应用于 7 份牛肾官方对照样品。使用 CBS-MS 方法检测发现其中一个样品呈阳性,经 LC-MSMS 分析确认为真阳性。所开发的方法表明,CBS 设备可直接提取和分析肾脏样品,用于食品安全检测。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rapid identification of antibiotic residues in bovine kidney using coated blade spray-mass spectrometry.

The use of certain antibiotics in food-producing animals is allowed in Europe following Regulation (EU) 2017/625. However, use could result in antibiotic residues in foodstuffs of animal origin. Maximum residue limits (MRLs) are in place to protect consumers. For monitoring purposes, animal matrices are tested to verify their compliance with these MRLs. Initially, matrices of (slaughtered) food animals are screened, often using a microbiological assay. Faster screening tests for antibiotics would be an advantage for control laboratories. Therefore, the present study describes, for the first time, the use of coated blade spray (CBS) followed by direct mass spectrometry (MS) analysis for the screening of tetracyclines, sulfonamides, quinolones, and macrolides residues from the renal area of intact bovine kidneys. An optimized workflow using two different desorption/ionization solutions per blade allowed screening of target compounds within 1 min per sample. The proof-of-principle of the CBS-MS method is validated according to (EU) 2021/808, presenting CCβ screening values of 0.1 × MRL for 43 analytes, 0.5 × MRL for 4 analytes, and 2.5 µg kg-1 for the prohibited substance dapsone, respectively. The developed method was successfully applied to seven official control samples of bovine kidneys. One of these samples was found to be positive using the CBS-MS method, which was confirmed as a true positive by LC-MSMS analysis. The developed method demonstrates that CBS devices can directly extract and analyze kidney samples for food safety testing.

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来源期刊
CiteScore
8.00
自引率
4.70%
发文量
638
审稿时长
2.1 months
期刊介绍: Analytical and Bioanalytical Chemistry’s mission is the rapid publication of excellent and high-impact research articles on fundamental and applied topics of analytical and bioanalytical measurement science. Its scope is broad, and ranges from novel measurement platforms and their characterization to multidisciplinary approaches that effectively address important scientific problems. The Editors encourage submissions presenting innovative analytical research in concept, instrumentation, methods, and/or applications, including: mass spectrometry, spectroscopy, and electroanalysis; advanced separations; analytical strategies in “-omics” and imaging, bioanalysis, and sampling; miniaturized devices, medical diagnostics, sensors; analytical characterization of nano- and biomaterials; chemometrics and advanced data analysis.
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