由 SgPG1 介导的边界样细胞形成赋予黔龙胆(Stylosanthes guianensis)抗铝性。

IF 6.2 1区 生物学 Q1 PLANT SCIENCES
Yan Lin, Guoxuan Liu, Pandao Liu, Qianqian Chen, Xueqiong Guo, Xing Lu, Zefei Cai, Lili Sun, Jiping Liu, Kang Chen, Guodao Liu, Jiang Tian, Cuiyue Liang
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引用次数: 0

摘要

木贼是热带地区的一种重要饲料豆科植物,对铝(Al)毒性具有很强的抗性,但对这种抗性的内在机制的了解仍然很零碎。我们发现,在所有 54 个受检的 Stylosanthes guianensis 基因型(但不包括 Stylosanthes viscose 基因型 TF0140)的根尖周围,都能持续产生边界样细胞(BLCs)。在铝条件下的基因型比较中,S. guianensis 基因型 RY#2 在 BLC 中保留的铝明显多于 TF0140,因此根的相对生长量也高于 TF0140。BLCs 的形成伴随着细胞壁果胶表位的变化和果胶代谢相关基因(包括聚半乳糖醛酸酶(SgPG1))的差异表达。SgPG1 的表达模式与 RY#2 和 TF0140 中 BLC 的形成一致。SgPG1 定位于细胞壁中,在介导去甲基酯化的均半乳糖醛酸降解方面表现出很高的活性。过量表达 SgPG1 可改变细胞壁果胶表位,提高 BLCs 产量,增强拟南芥和毛状根对 Al 的抗性。此外,结合体外和体内的蛋白质-DNA结合试验,证明 bHLH 转录因子 SgbHLH19 是 SgPG1 的上游调节因子。我们的研究表明,黔金丝猴的抗铝性主要依赖于BLCs,而BLCs的形成涉及细胞壁果胶表位的SgPG1修饰。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Border-like cell formation mediated by SgPG1 confers aluminum resistance in Stylosanthes guianensis

Stylosanthes is an important forage legume in tropical areas with strong resistance to aluminum (Al) toxicity, though knowledge of mechanisms underlying this resistance remains fragmentary. We found that border-like cells (BLCs) were constitutively produced surrounding the root tips of all 54 examined Stylosanthes guianensis genotypes, but not the Stylosanthes viscose genotype TF0140. In genotypic comparisons under Al conditions, the S. guianensis genotype RY#2 retained significantly more Al in BLCs and thereby showed higher relative root growth than TF0140. Formation of BLCs accompanied changes in cell wall pectin epitopes and differential expression of genes involved in pectin metabolism, including a polygalacturonase (SgPG1). The expression pattern of SgPG1 was consistent with the formation of BLCs in both RY#2 and TF0140. SgPG1 was localized in cell walls and exhibited high activities mediating demethyl-esterified homogalacturonan degradation. Overexpressing SgPG1 changed cell wall pectin epitopes, enhanced BLCs production, and Al resistance in both Arabidopsis and Stylosanthes hairy roots. Furthermore, combining protein-DNA binding assays in vitro and in vivo, a bHLH transcription factor SgbHLH19 was demonstrated to be the upstream regulator of SgPG1. Our study demonstrates that S. guianensis Al resistance mainly relies on BLCs, whose formation involves cell wall pectin epitope modification by SgPG1.

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来源期刊
The Plant Journal
The Plant Journal 生物-植物科学
CiteScore
13.10
自引率
4.20%
发文量
415
审稿时长
2.3 months
期刊介绍: Publishing the best original research papers in all key areas of modern plant biology from the world"s leading laboratories, The Plant Journal provides a dynamic forum for this ever growing international research community. Plant science research is now at the forefront of research in the biological sciences, with breakthroughs in our understanding of fundamental processes in plants matching those in other organisms. The impact of molecular genetics and the availability of model and crop species can be seen in all aspects of plant biology. For publication in The Plant Journal the research must provide a highly significant new contribution to our understanding of plants and be of general interest to the plant science community.
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