在根结线虫与番茄植株的相互作用过程中,miR396a-SlGRF8 模块通过 SlSTP10 调节根部的糖积累。

IF 9.3 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Lulu Sun, Mengting Zhu, Xiaoxuan Zhou, Ruiyue Gu, Yuying Hou, Tongtong Li, Huang Huang, Rui Yang, Shaohui Wang, Wenchao Zhao
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引用次数: 0

摘要

根结线虫(RKNs;Meloidogyne spp.)对作物生产构成严重威胁。植物和病原体之间对同化物的竞争影响着它们相互作用的结果。然而,植物与线虫之间竞争同化物的机制尚未阐明。在这项研究中,我们证明了 miR396a 在防御 RKNs 的过程中起负作用,而在番茄根部的糖积累过程中起正作用。miR396a的靶标SlGRF8(番茄生长调节因子8)的过表达降低了根的含糖量和对RKNs的敏感性,而grf8-cr突变则产生了相反的效果。此外,我们证实 SlGRF8 在 RKN 胁迫下通过直接激活 SlSTP10(番茄糖转运蛋白 10)的转录来调节根的含糖量。此外,SlSTP10 主要在巨细胞周围的组织中表达,SlSTP10 基因敲除既增加了根中的含糖量,也增加了植物对 RKN 的敏感性。总之,这项研究为了解 miR396a-SlGRF8-SlSTP10 模块调控 RKN 胁迫下根中糖分配的分子机制提供了重要的启示。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The miR396a–SlGRF8 module regulates sugar accumulation in the roots via SlSTP10 during the interaction between root-knot nematodes and tomato plants

The miR396a–SlGRF8 module regulates sugar accumulation in the roots via SlSTP10 during the interaction between root-knot nematodes and tomato plants

Root-knot nematodes (RKNs; Meloidogyne spp.) are a serious threat to crop production. The competition between plants and pathogens for assimilates influences the outcome of their interactions. However, the mechanisms by which plants and nematodes compete with each other for assimilates have not been elucidated. In this study, we demonstrated that miR396a plays a negative role in defense against RKNs and a positive role in sugar accumulation in tomato roots. The overexpression of SlGRF8 (Solanum lycopersicum growth-regulating factor 8), the target of miR396a, decreased the sugar content of the roots and the susceptibility to RKNs, whereas the grf8-cr mutation had the opposite effects. Furthermore, we confirmed that SlGRF8 regulated the sugar content in roots by directly activating the transcription of SlSTP10 (Solanum lycopersicum sugar transporter protein 10) in response to RKN stress. Moreover, SlSTP10 was expressed primarily in the tissues surrounding giant cells, and the SlSTP10 knockout increased both the sugar content in the roots and the plant's susceptibility to RKNs. Overall, this study provides important insight into the molecular mechanism through which the miR396a-SlGRF8-SlSTP10 module regulates sugar allocation in roots under RKN stress.

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来源期刊
Journal of Integrative Plant Biology
Journal of Integrative Plant Biology 生物-生化与分子生物学
CiteScore
18.00
自引率
5.30%
发文量
220
审稿时长
3 months
期刊介绍: Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.
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