Cas9-Trex2融合蛋白在多倍体基因组中的高效基因干扰。

IF 9.3 1区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Integrative Plant Biology Pub Date : 2024-10-25 DOI:10.1111/jipb.13797

Wenbo Pan, Chunlei Gao, De Niu, Jinghua Cheng, Jiao Zhang, Xiying Yan, Qiang Long, YaoYao Zhu, Wenjing Sun, Qi Xie, Yuehui He, Xing Wang Deng, Huawei Zhang, Jian Li

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引用次数: 0

摘要

外切酶 Trex2 与 Cas9 蛋白的融合大大提高了六倍体普通小麦基因组编辑的效率，特别是在单代内同时编辑多个有利等位基因的效率，从而促进了多倍体作物的基因组编辑辅助育种。

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Efficient gene disruption in polyploid genome by Cas9-Trex2 fusion protein.

The fusion of the exonuclease Trex2 with the Cas9 protein significantly enhanced the efficiency of genome editing in hexaploid common wheat, particularly for the simultaneous editing of multiple favorable alleles within a single generation, thereby facilitating genome editing-assisted breeding in polyploid crops.

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来源期刊

Journal of Integrative Plant Biology 生物-生化与分子生物学

CiteScore

18.00

自引率

5.30%

发文量

220

审稿时长

3 months

期刊介绍： Journal of Integrative Plant Biology is a leading academic journal reporting on the latest discoveries in plant biology.Enjoy the latest news and developments in the field, understand new and improved methods and research tools, and explore basic biological questions through reproducible experimental design, using genetic, biochemical, cell and molecular biological methods, and statistical analyses.