Xuechao Wang, Thomas J. Browning, Eric P. Achterberg, Martha Gledhill
{"title":"在无机磷源和有机磷源条件下生长的受限铁三藻的不同元素化学结构","authors":"Xuechao Wang, Thomas J. Browning, Eric P. Achterberg, Martha Gledhill","doi":"10.1002/lno.12716","DOIUrl":null,"url":null,"abstract":"<jats:italic>Trichodesmium</jats:italic> spp. is a colonial diazotrophic cyanobacterium found in the oligotrophic (sub)tropical oceans, where its distribution is strongly regulated by the availability of phosphorus and iron. The bulk carbon : nitrogen : phosphorus elemental composition of phytoplankton has previously been shown to depart from classical “Redfield” values under nutrient‐limitation conditions. We hypothesized that the abundance of intracellular metabolites and the extended Redfield ratios of <jats:italic>Trichodesmium</jats:italic>, including a range of trace elements, are variable in response to conditions of phosphorus and iron limitation that are found in the ocean. To test this, we grew <jats:italic>Trichodesmium</jats:italic> under trace metal–controlled conditions, where phosphorus was supplied as either dissolved inorganic phosphorus (DIP) or dissolved organic phosphorus (DOP) from iron depleted to elevated levels. We found that the steady‐state extended Redfield ratios of <jats:italic>Trichodesmium</jats:italic> under the iron‐depleted condition was (C<jats:sub>106</jats:sub>N<jats:sub>15.82</jats:sub>P<jats:sub>0.62</jats:sub>)<jats:sub>1000</jats:sub>Fe<jats:sub>2.26</jats:sub>Zn<jats:sub>2.37</jats:sub>Mn<jats:sub>1.68</jats:sub>Cu<jats:sub>0.68</jats:sub>Ni<jats:sub>0.31</jats:sub>Mo<jats:sub>0.42</jats:sub>Co<jats:sub>0.03</jats:sub> for the DIP treatment where <jats:italic>Trichodesmium</jats:italic> was under iron limitation, and (C<jats:sub>106</jats:sub>N<jats:sub>13.89</jats:sub>P<jats:sub>0.49</jats:sub>)<jats:sub>1000</jats:sub>Fe<jats:sub>3.38</jats:sub>Zn<jats:sub>2.51</jats:sub>Mn<jats:sub>0.97</jats:sub>Cu<jats:sub>0.52</jats:sub>Ni<jats:sub>0.42</jats:sub>Mo<jats:sub>0.33</jats:sub>Co<jats:sub>0.03</jats:sub> for the DOP treatment where <jats:italic>Trichodesmium</jats:italic> was under iron–phosphorus co‐limitation. Mean steady‐state cellular iron : carbon in the DIP treatment (iron limited) was only 50% of that in the control treatment, while zinc : carbon was elevated twofold. The average extended Redfield ratios following recovery from iron limitation was (C<jats:sub>106</jats:sub>N<jats:sub>16.8</jats:sub>P<jats:sub>0.7</jats:sub>)<jats:sub>1000</jats:sub>Fe<jats:sub>4.41</jats:sub>Zn<jats:sub>1.44</jats:sub>Mn<jats:sub>1</jats:sub>Cu<jats:sub>0.52</jats:sub>Ni<jats:sub>0.19</jats:sub>Mo<jats:sub>0.3</jats:sub>Co<jats:sub>0.03</jats:sub> for the DIP and (C<jats:sub>106</jats:sub>N<jats:sub>15.9</jats:sub>P<jats:sub>0.73</jats:sub>)<jats:sub>1000</jats:sub>Fe<jats:sub>7.36</jats:sub>Zn<jats:sub>2.24</jats:sub>Mn<jats:sub>1.08</jats:sub>Cu<jats:sub>0.71</jats:sub>Ni<jats:sub>0.63</jats:sub>Mo<jats:sub>0.38</jats:sub>Co<jats:sub>0.02</jats:sub> for the DOP treatment. No significant changes were observed in the carbon‐normalized abundance of targeted metabolites produced by <jats:italic>Trichodesmium</jats:italic>, under the different treatments. These results suggest <jats:italic>Trichodesmium</jats:italic> employs different strategies to cope with iron/phosphorus limitation, which is reflected in its extended Redfield ratios.","PeriodicalId":18143,"journal":{"name":"Limnology and Oceanography","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Different elemental stoichiometries of Fe‐limited Trichodesmium when grown under inorganic and organic phosphorus sources\",\"authors\":\"Xuechao Wang, Thomas J. Browning, Eric P. Achterberg, Martha Gledhill\",\"doi\":\"10.1002/lno.12716\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<jats:italic>Trichodesmium</jats:italic> spp. is a colonial diazotrophic cyanobacterium found in the oligotrophic (sub)tropical oceans, where its distribution is strongly regulated by the availability of phosphorus and iron. The bulk carbon : nitrogen : phosphorus elemental composition of phytoplankton has previously been shown to depart from classical “Redfield” values under nutrient‐limitation conditions. We hypothesized that the abundance of intracellular metabolites and the extended Redfield ratios of <jats:italic>Trichodesmium</jats:italic>, including a range of trace elements, are variable in response to conditions of phosphorus and iron limitation that are found in the ocean. To test this, we grew <jats:italic>Trichodesmium</jats:italic> under trace metal–controlled conditions, where phosphorus was supplied as either dissolved inorganic phosphorus (DIP) or dissolved organic phosphorus (DOP) from iron depleted to elevated levels. We found that the steady‐state extended Redfield ratios of <jats:italic>Trichodesmium</jats:italic> under the iron‐depleted condition was (C<jats:sub>106</jats:sub>N<jats:sub>15.82</jats:sub>P<jats:sub>0.62</jats:sub>)<jats:sub>1000</jats:sub>Fe<jats:sub>2.26</jats:sub>Zn<jats:sub>2.37</jats:sub>Mn<jats:sub>1.68</jats:sub>Cu<jats:sub>0.68</jats:sub>Ni<jats:sub>0.31</jats:sub>Mo<jats:sub>0.42</jats:sub>Co<jats:sub>0.03</jats:sub> for the DIP treatment where <jats:italic>Trichodesmium</jats:italic> was under iron limitation, and (C<jats:sub>106</jats:sub>N<jats:sub>13.89</jats:sub>P<jats:sub>0.49</jats:sub>)<jats:sub>1000</jats:sub>Fe<jats:sub>3.38</jats:sub>Zn<jats:sub>2.51</jats:sub>Mn<jats:sub>0.97</jats:sub>Cu<jats:sub>0.52</jats:sub>Ni<jats:sub>0.42</jats:sub>Mo<jats:sub>0.33</jats:sub>Co<jats:sub>0.03</jats:sub> for the DOP treatment where <jats:italic>Trichodesmium</jats:italic> was under iron–phosphorus co‐limitation. Mean steady‐state cellular iron : carbon in the DIP treatment (iron limited) was only 50% of that in the control treatment, while zinc : carbon was elevated twofold. The average extended Redfield ratios following recovery from iron limitation was (C<jats:sub>106</jats:sub>N<jats:sub>16.8</jats:sub>P<jats:sub>0.7</jats:sub>)<jats:sub>1000</jats:sub>Fe<jats:sub>4.41</jats:sub>Zn<jats:sub>1.44</jats:sub>Mn<jats:sub>1</jats:sub>Cu<jats:sub>0.52</jats:sub>Ni<jats:sub>0.19</jats:sub>Mo<jats:sub>0.3</jats:sub>Co<jats:sub>0.03</jats:sub> for the DIP and (C<jats:sub>106</jats:sub>N<jats:sub>15.9</jats:sub>P<jats:sub>0.73</jats:sub>)<jats:sub>1000</jats:sub>Fe<jats:sub>7.36</jats:sub>Zn<jats:sub>2.24</jats:sub>Mn<jats:sub>1.08</jats:sub>Cu<jats:sub>0.71</jats:sub>Ni<jats:sub>0.63</jats:sub>Mo<jats:sub>0.38</jats:sub>Co<jats:sub>0.02</jats:sub> for the DOP treatment. No significant changes were observed in the carbon‐normalized abundance of targeted metabolites produced by <jats:italic>Trichodesmium</jats:italic>, under the different treatments. These results suggest <jats:italic>Trichodesmium</jats:italic> employs different strategies to cope with iron/phosphorus limitation, which is reflected in its extended Redfield ratios.\",\"PeriodicalId\":18143,\"journal\":{\"name\":\"Limnology and Oceanography\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.8000,\"publicationDate\":\"2024-10-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Limnology and Oceanography\",\"FirstCategoryId\":\"89\",\"ListUrlMain\":\"https://doi.org/10.1002/lno.12716\",\"RegionNum\":1,\"RegionCategory\":\"地球科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"LIMNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Limnology and Oceanography","FirstCategoryId":"89","ListUrlMain":"https://doi.org/10.1002/lno.12716","RegionNum":1,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"LIMNOLOGY","Score":null,"Total":0}
Different elemental stoichiometries of Fe‐limited Trichodesmium when grown under inorganic and organic phosphorus sources
Trichodesmium spp. is a colonial diazotrophic cyanobacterium found in the oligotrophic (sub)tropical oceans, where its distribution is strongly regulated by the availability of phosphorus and iron. The bulk carbon : nitrogen : phosphorus elemental composition of phytoplankton has previously been shown to depart from classical “Redfield” values under nutrient‐limitation conditions. We hypothesized that the abundance of intracellular metabolites and the extended Redfield ratios of Trichodesmium, including a range of trace elements, are variable in response to conditions of phosphorus and iron limitation that are found in the ocean. To test this, we grew Trichodesmium under trace metal–controlled conditions, where phosphorus was supplied as either dissolved inorganic phosphorus (DIP) or dissolved organic phosphorus (DOP) from iron depleted to elevated levels. We found that the steady‐state extended Redfield ratios of Trichodesmium under the iron‐depleted condition was (C106N15.82P0.62)1000Fe2.26Zn2.37Mn1.68Cu0.68Ni0.31Mo0.42Co0.03 for the DIP treatment where Trichodesmium was under iron limitation, and (C106N13.89P0.49)1000Fe3.38Zn2.51Mn0.97Cu0.52Ni0.42Mo0.33Co0.03 for the DOP treatment where Trichodesmium was under iron–phosphorus co‐limitation. Mean steady‐state cellular iron : carbon in the DIP treatment (iron limited) was only 50% of that in the control treatment, while zinc : carbon was elevated twofold. The average extended Redfield ratios following recovery from iron limitation was (C106N16.8P0.7)1000Fe4.41Zn1.44Mn1Cu0.52Ni0.19Mo0.3Co0.03 for the DIP and (C106N15.9P0.73)1000Fe7.36Zn2.24Mn1.08Cu0.71Ni0.63Mo0.38Co0.02 for the DOP treatment. No significant changes were observed in the carbon‐normalized abundance of targeted metabolites produced by Trichodesmium, under the different treatments. These results suggest Trichodesmium employs different strategies to cope with iron/phosphorus limitation, which is reflected in its extended Redfield ratios.
期刊介绍:
Limnology and Oceanography (L&O; print ISSN 0024-3590, online ISSN 1939-5590) publishes original articles, including scholarly reviews, about all aspects of limnology and oceanography. The journal''s unifying theme is the understanding of aquatic systems. Submissions are judged on the originality of their data, interpretations, and ideas, and on the degree to which they can be generalized beyond the particular aquatic system examined. Laboratory and modeling studies must demonstrate relevance to field environments; typically this means that they are bolstered by substantial "real-world" data. Few purely theoretical or purely empirical papers are accepted for review.