Kyle R. Barrie, Grzegorz Rebowski, Roberto Dominguez
{"title":"凝胶球蛋白切断和覆盖肌动蛋白丝的机制","authors":"Kyle R. Barrie, Grzegorz Rebowski, Roberto Dominguez","doi":"10.1038/s41594-024-01412-5","DOIUrl":null,"url":null,"abstract":"<p>Gelsolin is the prototypical member of a family of Ca<sup>2+</sup>-activated F-actin severing and capping proteins. Here we report structures of Ca<sup>2+</sup>-bound human gelsolin at the barbed end of F-actin. One structure reveals gelsolin’s six domains (G1G6) and interdomain linkers wrapping around F-actin, while another shows domains G1G3—a fragment observed during apoptosis—binding on both sides of F-actin. Conformational changes that trigger severing occur on one side of F-actin with G1G6 and on both sides with G1G3. Gelsolin remains bound after severing, blocking subunit exchange.</p>","PeriodicalId":18822,"journal":{"name":"Nature structural & molecular biology","volume":"40 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mechanism of actin filament severing and capping by gelsolin\",\"authors\":\"Kyle R. Barrie, Grzegorz Rebowski, Roberto Dominguez\",\"doi\":\"10.1038/s41594-024-01412-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Gelsolin is the prototypical member of a family of Ca<sup>2+</sup>-activated F-actin severing and capping proteins. Here we report structures of Ca<sup>2+</sup>-bound human gelsolin at the barbed end of F-actin. One structure reveals gelsolin’s six domains (G1G6) and interdomain linkers wrapping around F-actin, while another shows domains G1G3—a fragment observed during apoptosis—binding on both sides of F-actin. Conformational changes that trigger severing occur on one side of F-actin with G1G6 and on both sides with G1G3. Gelsolin remains bound after severing, blocking subunit exchange.</p>\",\"PeriodicalId\":18822,\"journal\":{\"name\":\"Nature structural & molecular biology\",\"volume\":\"40 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-10-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nature structural & molecular biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1038/s41594-024-01412-5\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature structural & molecular biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1038/s41594-024-01412-5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Mechanism of actin filament severing and capping by gelsolin
Gelsolin is the prototypical member of a family of Ca2+-activated F-actin severing and capping proteins. Here we report structures of Ca2+-bound human gelsolin at the barbed end of F-actin. One structure reveals gelsolin’s six domains (G1G6) and interdomain linkers wrapping around F-actin, while another shows domains G1G3—a fragment observed during apoptosis—binding on both sides of F-actin. Conformational changes that trigger severing occur on one side of F-actin with G1G6 and on both sides with G1G3. Gelsolin remains bound after severing, blocking subunit exchange.
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