{"title":"一种超灵敏液晶光传感芯片,由三向结 DNA 袋辅助,用于检测食品样品中的丙烯酰胺","authors":"Zahra Khoshbin, Fatemeh Mohammadi, Marzieh Moeenfard, Khalil Abnous, Seyed Mohammad Taghdisi","doi":"10.1016/j.jhazmat.2024.136240","DOIUrl":null,"url":null,"abstract":"Acrylamide, an unsaturated amide found in heat-processed foods, poses serious risks to human health due to its neurotoxicity, carcinogenicity, and genotoxicity. This highlights the importance of quantitative monitoring of acrylamide in foods and the environments to ensure public health safety. Therefore, there is an urgent need for simple, rapid, and highly sensitive methods to accurately quantify acrylamide. In the present study, a user-friendly aptasensor was designed to quantify ultra-low levels of acrylamide in nuts for the first time. This innovative approach utilizes chemical engineering of a glass slide as a portable sensing platform, which incorporates liquid crystal (LC) molecules and a three-way junction (TWJ) DNA pocket. The immobilized TWJ pocket can disrupts the vertical alignment of LCs, turning the dark polarized background of the aptasensor to a colorful state. The binding of the specific aptamer to acrylamide disrupts the TWJ structure, enabling the LCs to return to their homotropic alignment. This structural change restores the dark polarized view of the sensing platform. The TWJ-engineered LC aptasensor effectively detects ultra-low concentrations of acrylamide in the range of 0.0005 to 50 fmol/L, with a detection limit of 0.106 amol/L. The aptasensor was successfully applied to real roasted nut samples, including peanut, almond, pistachio, and hazelnut, achieving recovery values ranging from 96.84% to 99.61%. With its simplicity, portability, ease of use, and cost-effectiveness, this aptasensor is a powerful sensing device for food safety monitoring.<h3>Environmental implication</h3>Acrylamide is a thermal processing contaminant that forms in foods subjected to frying, toasting, and roasting. Furthermore, its high solubility in water raises significant concerns about environmental contamination, particularly through industrial effluents and agricultural runoff. Given the health risks associated with acrylamide, such as neurotoxicity, carcinogenicity, and genotoxicity, there is an urgent need for a sensitive, specific, and rapid technique to accurately determi ne its content in both food and the environment. To address this need, we introduced a simple, rapid, and cost-effective aptasensing strategy. This approach involves the chemical engineering of a glassy slide as a portable sensing platform, incorporating liquid crystal molecules and a three-way junction DNA pocket for efficient detection.","PeriodicalId":361,"journal":{"name":"Journal of Hazardous Materials","volume":null,"pages":null},"PeriodicalIF":12.2000,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An Ultrasensitive Liquid Crystal Aptasensing Chip Assisted by Three-Way Junction DNA Pockets for Acrylamide Detection in Food Samples\",\"authors\":\"Zahra Khoshbin, Fatemeh Mohammadi, Marzieh Moeenfard, Khalil Abnous, Seyed Mohammad Taghdisi\",\"doi\":\"10.1016/j.jhazmat.2024.136240\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Acrylamide, an unsaturated amide found in heat-processed foods, poses serious risks to human health due to its neurotoxicity, carcinogenicity, and genotoxicity. This highlights the importance of quantitative monitoring of acrylamide in foods and the environments to ensure public health safety. Therefore, there is an urgent need for simple, rapid, and highly sensitive methods to accurately quantify acrylamide. In the present study, a user-friendly aptasensor was designed to quantify ultra-low levels of acrylamide in nuts for the first time. This innovative approach utilizes chemical engineering of a glass slide as a portable sensing platform, which incorporates liquid crystal (LC) molecules and a three-way junction (TWJ) DNA pocket. The immobilized TWJ pocket can disrupts the vertical alignment of LCs, turning the dark polarized background of the aptasensor to a colorful state. The binding of the specific aptamer to acrylamide disrupts the TWJ structure, enabling the LCs to return to their homotropic alignment. This structural change restores the dark polarized view of the sensing platform. The TWJ-engineered LC aptasensor effectively detects ultra-low concentrations of acrylamide in the range of 0.0005 to 50 fmol/L, with a detection limit of 0.106 amol/L. The aptasensor was successfully applied to real roasted nut samples, including peanut, almond, pistachio, and hazelnut, achieving recovery values ranging from 96.84% to 99.61%. With its simplicity, portability, ease of use, and cost-effectiveness, this aptasensor is a powerful sensing device for food safety monitoring.<h3>Environmental implication</h3>Acrylamide is a thermal processing contaminant that forms in foods subjected to frying, toasting, and roasting. Furthermore, its high solubility in water raises significant concerns about environmental contamination, particularly through industrial effluents and agricultural runoff. Given the health risks associated with acrylamide, such as neurotoxicity, carcinogenicity, and genotoxicity, there is an urgent need for a sensitive, specific, and rapid technique to accurately determi ne its content in both food and the environment. To address this need, we introduced a simple, rapid, and cost-effective aptasensing strategy. 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An Ultrasensitive Liquid Crystal Aptasensing Chip Assisted by Three-Way Junction DNA Pockets for Acrylamide Detection in Food Samples
Acrylamide, an unsaturated amide found in heat-processed foods, poses serious risks to human health due to its neurotoxicity, carcinogenicity, and genotoxicity. This highlights the importance of quantitative monitoring of acrylamide in foods and the environments to ensure public health safety. Therefore, there is an urgent need for simple, rapid, and highly sensitive methods to accurately quantify acrylamide. In the present study, a user-friendly aptasensor was designed to quantify ultra-low levels of acrylamide in nuts for the first time. This innovative approach utilizes chemical engineering of a glass slide as a portable sensing platform, which incorporates liquid crystal (LC) molecules and a three-way junction (TWJ) DNA pocket. The immobilized TWJ pocket can disrupts the vertical alignment of LCs, turning the dark polarized background of the aptasensor to a colorful state. The binding of the specific aptamer to acrylamide disrupts the TWJ structure, enabling the LCs to return to their homotropic alignment. This structural change restores the dark polarized view of the sensing platform. The TWJ-engineered LC aptasensor effectively detects ultra-low concentrations of acrylamide in the range of 0.0005 to 50 fmol/L, with a detection limit of 0.106 amol/L. The aptasensor was successfully applied to real roasted nut samples, including peanut, almond, pistachio, and hazelnut, achieving recovery values ranging from 96.84% to 99.61%. With its simplicity, portability, ease of use, and cost-effectiveness, this aptasensor is a powerful sensing device for food safety monitoring.
Environmental implication
Acrylamide is a thermal processing contaminant that forms in foods subjected to frying, toasting, and roasting. Furthermore, its high solubility in water raises significant concerns about environmental contamination, particularly through industrial effluents and agricultural runoff. Given the health risks associated with acrylamide, such as neurotoxicity, carcinogenicity, and genotoxicity, there is an urgent need for a sensitive, specific, and rapid technique to accurately determi ne its content in both food and the environment. To address this need, we introduced a simple, rapid, and cost-effective aptasensing strategy. This approach involves the chemical engineering of a glassy slide as a portable sensing platform, incorporating liquid crystal molecules and a three-way junction DNA pocket for efficient detection.
期刊介绍:
The Journal of Hazardous Materials serves as a global platform for promoting cutting-edge research in the field of Environmental Science and Engineering. Our publication features a wide range of articles, including full-length research papers, review articles, and perspectives, with the aim of enhancing our understanding of the dangers and risks associated with various materials concerning public health and the environment. It is important to note that the term "environmental contaminants" refers specifically to substances that pose hazardous effects through contamination, while excluding those that do not have such impacts on the environment or human health. Moreover, we emphasize the distinction between wastes and hazardous materials in order to provide further clarity on the scope of the journal. We have a keen interest in exploring specific compounds and microbial agents that have adverse effects on the environment.