{"title":"正常孕妇和妊娠糖尿病孕妇离体脐动脉中的 Toll 样受体 4 (TLR4) 对血管紧张素 II 的决定性作用","authors":"Esra Büyük Gezer, Ayşe Saide Şahin","doi":"10.5543/tkda.2024.00273","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>Gestational diabetes mellitus (GDM) is a common condition that occurs during pregnancy and results in cesarean section, pre-eclampsia, and neonatal morbidity. Angiotensin II is a potent vasoconstrictor and an important determinant of uteroplacental perfusion. Toll-like receptor 4 (TLR4) was found to contribute to diabetes progression. This study aimed to determine how TLR4 activation affects the contraction mediated by angiotensin II type 1 receptor in the isolated umbilical arteries of normal and GDM women.</p><p><strong>Methods: </strong>Angiotensin II was applied with either a TLR4 agonist or TLR4 antagonists to isolated arteries from normal and GDM umbilical cords. The changes in the angiotensin II response were expressed as the maximal contraction percentage and pD2 values. The mRNA expression levels of TLR4 and angiotensin II type 1 receptor gene were measured via quantitative real-time polymerase chain reaction analysis.</p><p><strong>Results: </strong>This study displayed an increased sensitivity to angiotensin II in the arteries of the GDM group compared with the normal group. The TLR4 agonist showed a synergistic effect with angiotensin II, while the TLR4 antagonists were not strongly determinant. In the GDM group, the TLR4 mRNA level is slightly higher than in the normal pregnancy group; however, no statistical difference was noted.</p><p><strong>Conclusion: </strong>Toll-like receptor 4 may determine the vasoconstrictive effect of angiotensin II in normal and GDM umbilical arteries. Some studies showed a similar interaction as supportive. However, comprehensive animal and/or human studies are promising.</p>","PeriodicalId":94261,"journal":{"name":"Turk Kardiyoloji Dernegi arsivi : Turk Kardiyoloji Derneginin yayin organidir","volume":"52 7","pages":"486-491"},"PeriodicalIF":0.0000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Determinant Role of Toll-like Receptor 4 (TLR4) on Angiotensin II in Isolated Umbilical Arteries from Normal and Gestational Diabetes Pregnant Women.\",\"authors\":\"Esra Büyük Gezer, Ayşe Saide Şahin\",\"doi\":\"10.5543/tkda.2024.00273\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Gestational diabetes mellitus (GDM) is a common condition that occurs during pregnancy and results in cesarean section, pre-eclampsia, and neonatal morbidity. Angiotensin II is a potent vasoconstrictor and an important determinant of uteroplacental perfusion. Toll-like receptor 4 (TLR4) was found to contribute to diabetes progression. This study aimed to determine how TLR4 activation affects the contraction mediated by angiotensin II type 1 receptor in the isolated umbilical arteries of normal and GDM women.</p><p><strong>Methods: </strong>Angiotensin II was applied with either a TLR4 agonist or TLR4 antagonists to isolated arteries from normal and GDM umbilical cords. The changes in the angiotensin II response were expressed as the maximal contraction percentage and pD2 values. The mRNA expression levels of TLR4 and angiotensin II type 1 receptor gene were measured via quantitative real-time polymerase chain reaction analysis.</p><p><strong>Results: </strong>This study displayed an increased sensitivity to angiotensin II in the arteries of the GDM group compared with the normal group. The TLR4 agonist showed a synergistic effect with angiotensin II, while the TLR4 antagonists were not strongly determinant. In the GDM group, the TLR4 mRNA level is slightly higher than in the normal pregnancy group; however, no statistical difference was noted.</p><p><strong>Conclusion: </strong>Toll-like receptor 4 may determine the vasoconstrictive effect of angiotensin II in normal and GDM umbilical arteries. Some studies showed a similar interaction as supportive. 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引用次数: 0
摘要
目的:妊娠期糖尿病(GDM)是孕期常见病,可导致剖宫产、先兆子痫和新生儿发病率。血管紧张素 II 是一种强效的血管收缩剂,是决定子宫胎盘灌注的重要因素。研究发现,Toll 样受体 4(TLR4)有助于糖尿病的发展。本研究旨在确定 TLR4 激活如何影响正常和 GDM 妇女离体脐动脉中血管紧张素 II 1 型受体介导的收缩:将血管紧张素 II 与 TLR4 激动剂或 TLR4 拮抗剂一起作用于正常和 GDM 脐带离体动脉。血管紧张素 II 反应的变化以最大收缩百分比和 pD2 值表示。通过定量实时聚合酶链反应分析测定了 TLR4 和血管紧张素 II 1 型受体基因的 mRNA 表达水平:结果:该研究显示,与正常组相比,GDM 组动脉对血管紧张素 II 的敏感性增加。TLR4 激动剂与血管紧张素 II 有协同作用,而 TLR4 拮抗剂的决定作用不强。在 GDM 组中,TLR4 mRNA 水平略高于正常妊娠组,但无统计学差异:结论:在正常和 GDM 脐动脉中,Toll 样受体 4 可能决定血管紧张素 II 的血管收缩效应。一些研究显示了与支持性研究类似的相互作用。然而,全面的动物和/或人体研究很有希望。
Determinant Role of Toll-like Receptor 4 (TLR4) on Angiotensin II in Isolated Umbilical Arteries from Normal and Gestational Diabetes Pregnant Women.
Objective: Gestational diabetes mellitus (GDM) is a common condition that occurs during pregnancy and results in cesarean section, pre-eclampsia, and neonatal morbidity. Angiotensin II is a potent vasoconstrictor and an important determinant of uteroplacental perfusion. Toll-like receptor 4 (TLR4) was found to contribute to diabetes progression. This study aimed to determine how TLR4 activation affects the contraction mediated by angiotensin II type 1 receptor in the isolated umbilical arteries of normal and GDM women.
Methods: Angiotensin II was applied with either a TLR4 agonist or TLR4 antagonists to isolated arteries from normal and GDM umbilical cords. The changes in the angiotensin II response were expressed as the maximal contraction percentage and pD2 values. The mRNA expression levels of TLR4 and angiotensin II type 1 receptor gene were measured via quantitative real-time polymerase chain reaction analysis.
Results: This study displayed an increased sensitivity to angiotensin II in the arteries of the GDM group compared with the normal group. The TLR4 agonist showed a synergistic effect with angiotensin II, while the TLR4 antagonists were not strongly determinant. In the GDM group, the TLR4 mRNA level is slightly higher than in the normal pregnancy group; however, no statistical difference was noted.
Conclusion: Toll-like receptor 4 may determine the vasoconstrictive effect of angiotensin II in normal and GDM umbilical arteries. Some studies showed a similar interaction as supportive. However, comprehensive animal and/or human studies are promising.