紫花苜蓿（Medicago sativa L.）绿色生物质衍生部分的水溶性有机硒代谢物。

IF 3.6 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Trace Elements in Medicine and Biology Pub Date : 2024-10-10 DOI:10.1016/j.jtemb.2024.127545

Éva Domokos‑Szabolcsy , Áron Soós , Béla Kovács , Zoltán Kovács , Mihály Dernovics

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引用次数: 0

摘要

背景：硒是高等植物非必需的微量元素，植物对硒的耐受性是设计间接（高硒土壤的硒消耗）或直接（硒化饲料生产）富硒饲草时的一个关键问题。紫花苜蓿（Medicago sativa L.）是著名的豆科牧草作物，因其可用作绿肥、覆盖作物或通过固氮作用进行土壤修复而备受关注：我们研究的目的是评估紫花苜蓿植物从土壤中吸收的过量硒通过生物转化转化为哪些硒化合物。硒化合物（硒蛋氨酸和无机形式除外）在苜蓿生物质纤维和所谓的褐汁副产品中积累：方法：采用电感耦合等离子体-质谱法辅助多维（强阴离子交换、强阳离子交换、反相）正交色谱纯化，为电喷雾高分辨质谱（首次用于该基质）分析提供足够高纯度的含硒馏分：结果：总共描述了 30 种硒化合物（包括异构体），显示出硒代己糖、硒代荷包牡丹碱和 2,3-二羟基丙酸衍生物的丰富性。在 30 种硒化合物中，有 15 种可以确定元素组成，有 5 种化合物的结构可以确定，其中包括脱氨基-2-氧代-硒高茂肉毒碱、脱氨基-羟基-硒高茂肉毒碱和 2,3-二羟基丙酰基-硒高茂半胱氨酸的二聚体：所研究的苜蓿标准加工技术产生的馏分中含有多种硒化合物，其来源可能是植物固有的硒代谢，也可能是加工技术本身。在 30 种化合物中，有 16 种只能在电喷雾离子化模式下检测到，因此负模式数据采集的重要性得到了强调。

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Water-soluble organic selenometabolites of alfalfa (Medicago sativa L.) green biomass-derived fractions

Background

Tolerance of plants towards selenium, a non-essential microelement for higher plants, is a key issue when designing either the indirect (selenium-depletion from highly seleniferous soils) or directed (selenized feed production) enrichment of selenium in forages. Alfalfa (Medicago sativa L.), the well-known forage crop of the Fabaceae family, has been gaining considerable interest due to its application as a green manure, as a cover crop, or in soil remediation by nitrogen fixation.

Objective

The goal of our study was to assess into which selenocompounds alfalfa plants biotransform the excess selenium uptake from the soil. Selenocompounds (other than selenomethionine and inorganic forms) accumulated in the fiber and the so-called brown juice by-product fractions of processed alfalfa biomass were targeted.

Methodology

Inductively coupled plasma – mass spectrometry assisted multidimensional (strong anion exchange, strong cation exchange, reversed phase) orthogonal chromatographic purification was applied to supply Se-containing fractions in adequately high purity for electrospray high-resolution mass spectrometry (used for the first time for this matrix) analyses.

Results

As a total, 30 selenocompounds (with isomers) were described, showing the abundance of the derivatives of selenohexose, selenohomolanthionine, and 2,3-dihydroxypropionic acid. Out of the 30 selenocompounds, 15 could be assigned the elemental composition, and the tentative structure of five compounds including among others deamino-2-oxo-selenohomolanthionine, deamino-hydroxy-selenohomolanthionine, and the dimer of 2,3-dihydroxypropionyl-selenohomocysteine could be presented.

Conclusions

The studied fractions arising from the standard alfalfa processing technology contained a wide variety of selenocompounds whose origin can be either the plant’s inherent Se metabolism or the processing technology itself. The importance of negative mode data acquisition has been highlighted, as out of the 30 compounds, 16 could be detected exclusively in this electrospray ionization mode.

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来源期刊

Journal of Trace Elements in Medicine and Biology 医学-内分泌学与代谢

CiteScore

6.60

自引率

2.90%

发文量

202

审稿时长

85 days

期刊介绍： The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.