钼在改善硫丹诱导的雌性小鼠不孕症中的作用

IF 3.6 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Feng-jun Liu , Yu-ling Zhang , Xiao-shan Wang , Ya-qin Zhao , Hong-wei Wang
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引用次数: 0

摘要

背景:钼(Mo)在调节正常生理功能方面发挥着重要作用。然而,钼对女性不孕症的潜在影响却很少受到关注:本研究通过建立硫丹诱导的不孕症模型,探索钼对小鼠卵巢和卵母细胞作用的潜在分子机制。成年雌性昆明小鼠被随机分为三组:对照组、+硫丹组和+硫丹+莫组。硫丹+Mo组的小鼠在接受30天的硫丹治疗(美莱伦,20毫克/千克体重ip)后,每天在饮用水中添加7.5毫克/升的Mo,持续42天。第72天,我们用H&E染色法检测卵母细胞和卵巢组织的形态,用ELISA法测定血清激素的浓度,用免疫组化染色法和Western免疫印迹法检测Bax、Bcl-2、caspase-3和caspase-9。我们还用试剂盒测定了抗氧化酶 SOD 的活性、MDA 和 LDH 的浓度以及凋亡细胞的百分比,从而评估了细胞的氧化应激。与雄性小鼠交配后计算产仔数,用分析天平称重后计算器官系数:结果表明,Mo 治疗可使雌性生殖激素水平恢复到接近正常水平。Mo还能明显抑制线粒体应激诱导的凋亡蛋白的表达:我们的研究结果表明,7.5 mg/L剂量的Mo治疗可改善硫丹诱导的雌性小鼠不孕症。这些数据可为开发治疗雌性不孕症的方法提供参考。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of molybdenum in ameliorating busulfan-induced infertility in female mice

Background

Molybdenum (Mo) plays a crucial role in regulating normal physiological function. However, its potential effect on female infertility has received little attention.

Methods

In this study, we explored the potential molecular mechanisms of Mo’s action on mouse ovaries and oocytes by establishing a busulfan-induced infertility model. Adult female Kunming mice were randomly divided into three groups: control, +busulfan, and +busulfan+Mo. After 30 days of busulfan treatment [Myleran, 20 mg/kg body weight ip], mice in the busulfan+Mo group were provided with 7.5 mg/L Mo per day in drinking water for an additional 42 days. On day 72, we examined the morphology of the oocytes and ovarian tissue after H&E staining, measured the concentrations of serum hormones by ELISA, and detected Bax, Bcl-2, caspase-3 and caspase-9 by immunohistochemical staining and western immunoblotting. We also assessed the oxidative stress in cells by measuring the activity of the antioxidant enzyme, SOD, the concentrations of MDA and LDH, and the percentage of apoptotic cells using kits. The number of litters born was counted after mating with male mice, and the organ coefficients were calculated after weighing on an analytic balance.

Results

Results showed that Mo treatment restored female reproductive hormone levels to near normal. Mo also significantly inhibited the mitochondrial stress-induced expression of apoptotic proteins.

Conclusion

Our findings demonstrate that Mo treatment at a dose of 7.5 mg/L can ameliorate busulfan-induced infertility in female mice. These data may provide a reference for the development of treatments for female infertility.
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来源期刊
CiteScore
6.60
自引率
2.90%
发文量
202
审稿时长
85 days
期刊介绍: The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.
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