槲皮素通过抑制氧化应激和细胞凋亡减轻镉诱导的大鼠肝毒性

IF 3.6 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yaning Shi , Ke Wang , Hao Ling , Junbing Mao , Bing Xu , Zongping Liu , Jicang Wang
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引用次数: 0

摘要

背景:镉(Cd)被认为是一种主要的工业和环境有毒物质,威胁着水生生物、植物、动物和人类的健康。槲皮素(Que)是一种具有抗氧化特性的天然类黄酮。本研究旨在探讨氧化应激和细胞凋亡在镉诱导的肝毒性中的作用以及槲皮素的保护作用:将 36 只雄性 SD 大鼠随机分为 6 组:对照组、1 mg/kg Cd 组、2 mg/kg Cd 组、1 mg/kg Cd+Que 组、2 mg/kg Cd+Que 组和 Que 组。饲喂 28 天后,收集血清和肝组织样本,以评估肝功能、氧化应激水平、肝组织学和细胞凋亡:实验结果证实,与对照组相比,镉组的体重明显下降。此外,镉组的谷丙转氨酶(ALT)、谷草转氨酶(AST)和低密度脂蛋白胆碱酯酶(LDH)水平明显升高,SOD、CAT 活性和 GSH 含量明显降低,而 MDA 水平升高。肝脏病理切片显示,镉诱导的大鼠肝组织细胞破裂,细胞核外露,肝细胞索排列紊乱。镉暴露降低了 Nrf2 和 NQO1 的 mRNA 和蛋白表达,增加了 Keap1 的 mRNA 和蛋白表达,从而诱导氧化应激。同时,镉暴露会增加 Cytc、caspase-9、caspase-3 和 Bax 的 mRNA 和蛋白表达,而降低 Bcl-2 的表达。相反,加入阙后,镉诱导的肝损伤和氧化应激得到缓解,细胞凋亡得到抑制:总之,阙通过抑制氧化应激和细胞凋亡减轻了镉对肝脏的毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quercetin attenuates cadmium-induced hepatotoxicity by suppressing oxidative stress and apoptosis in rat

Background

Cadmium (Cd) is considered a major industrial and environmental toxicant, threatening the health of aquatic organisms, plants, animals, and humans. Quercetin (Que) is a natural flavonoid with antioxidant properties. The purpose of this study was to investigate the role of the oxidative stress and apoptosis in Cd-induced hepatotoxicity and the protective effect of Que.

Methodology

Thirty-six male SD rats were randomly divided into 6 groups: control group, 1 mg/kg Cd group, 2 mg/kg Cd group, 1 mg/kg Cd+Que group, 2 mg/kg Cd + Que group, and a Que group. After a feeding period of 28 days, serum and liver tissue samples were collected to evaluate liver function, oxidative stress levels, liver histology, and apoptosis.

Results

Experimental results confirmed that compared with the control group, the body weights of the Cd group significantly decreased. Additionally, there was a tremendous increased in the levels of ALT, AST, and LDH, and a significant decreased in the activities of SOD, CAT, and GSH content, while the level of MDA increased. Pathological sections of the liver showed that Cd-induced rats had ruptured liver tissue cells, exposed nuclei, and disturbed arrangement of hepatocyte cords. Cd exposure decreased the mRNA and protein expression of Nrf2 and NQO1 while increased the mRNA and protein expression of Keap1, thereby inducing oxidative stress. Meanwhile, Cd exposure increased the mRNA and protein expressions of Cytc, caspase-9, caspase-3, and Bax, while decreased the expression of Bcl-2. Conversely, after Que addition of alleviated liver injury and oxidative stress induced by Cd and inhibited apoptosis.

Conclusion

In conclusion, Que alleviates hepatic toxicity induced by Cd through suppression of oxidative stress and apoptosis.
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来源期刊
CiteScore
6.60
自引率
2.90%
发文量
202
审稿时长
85 days
期刊介绍: The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.
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