Erica L Romsos, Lisa A Borsuk, Carolyn R Steffen, Sarah Riman, Kevin M Kiesler, Peter M Vallone
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引用次数: 0
摘要
法医 DNA 分型技术和方法的进步提高了灵敏度,在带来好处的同时,也带来了更具挑战性的图谱解读。为此,法医 DNA 社区经常需要更复杂的参考材料,以解决常见的测量和解释问题,如复杂的 DNA 混合物、DNA 降解和 PCR 抑制等。美国国家标准与技术研究院(NIST)发布了研究级测试材料 10235:法医 DNA 分型资源样本》,为法医 DNA 界提供支持。样本包括三个单一来源样本、两个降解样本和三个混合物样本。作为研究级测试材料(RGTM)流程的一部分,研究了自动装瓶方法、替代样本管类型以及添加载体 RNA 以稳定低量样本的方法。通过 qPCR 测试,内部和外部测试都证明了材料在 4°C 温度下的长期稳定性。在数据门户网站的开发过程中,用户可以匿名上传结果,并将其数据与 NIST 和其他机构的数据进行比较。本报告介绍了这种材料的制备和稳定性。
Development of a forensic DNA research grade test material.
Advancements in forensic DNA typing technology and methods have increased sensitivity and, while beneficial, carry the weight of more challenging profile interpretation. In response, the forensic DNA community has often requested more complex reference materials to address commonly encountered measurement and interpretation issues such as complex DNA mixtures, DNA degradation, and PCR inhibition. The National Institute of Standards and Technology (NIST) released Research Grade Test Material 10235: Forensic DNA Typing Resource Samples to support the forensic DNA community. Components include three single source samples, two degraded samples, and three mixture samples. As part of the Research Grade Test Material (RGTM) process, automated methods for bottling, alternative sample tube types, and the addition of carrier RNA for stabilizing low-quantity samples were investigated. Both internal and external testing demonstrate the stability of the material over time at 4°C through qPCR testing. In the development of a data portal, users have been allowed to anonymously upload results and compare their data with NIST and others. This report describes the preparation and stability of this material.