用于关节缺损再生的全生物仿真透明软骨基质支架的构建与性能评估。

Haidi Sun, Zhonglian Wu, Lihua Liu, Xu Hu, Yurui Zhao, Chirun Wang, Jian Yang, Zehao Gu, Dong-An Wang, Hang Yao
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引用次数: 0

摘要

由于关节软骨中没有神经和血管,它的再生和修复在骨关节炎治疗中是一个重大而复杂的挑战。在软骨移植中,尤其是在寻求全面的生物仿生解决方案时,开发一种支持细胞生长的特殊物理和化学微环境一直是个难题。在以往研究的基础上,我们设计了一种组织工程脱细胞活体透明软骨移植体(dLhCG)。该研究开发了一种利用化学接枝技术提高支架亲水性和硬度的方法,并设计了一种用于组织工程的脱细胞透明软骨表型基质支架。在此,我们报告了一种使用1-乙基-3-(3-二甲氨基丙基)盐酸碳二亚胺/N-羟基琥珀酰亚胺(EDC/NHS)实现硫酸软骨素(CS)与dLhCG接枝的方法,最终产生了带有CS的组织工程化透明软骨移植物(dLhCG/CS)。杨氏模量测量结果表明,交联支架具有更强的机械性能。我们将交联的 dLhCG/CS 支架植入大鼠关节的蹄叶区,并通过组织学分析和生物力学测试评估其功能。12 周后,dLhCG/CS 支架表现出与 dLhCG 相当的优异生物诱导活性。再生组织有效地保持了透明软骨表型,并表现出相似的机械性能,在软骨再生中发挥了关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Construction and performance evaluation of fully biomimetic hyaline cartilage matrix scaffolds for joint defect regeneration.

Due to the absence of nerves and blood vessels in articular cartilage, its regeneration and repair present a significant and complex challenge in osteoarthritis treatment. Developing a specialized physical and chemical microenvironment supporting cell growth has been difficult in cartilage grafting, especially when aiming for comprehensive biomimetic solutions. Based on previous research, we have designed a tissue-engineered decellularized living hyaline cartilage graft (dLhCG). The study developed a method to improve the hydrophilicity and stiffness of scaffolds by employing chemical grafting techniques and designed a decellularized hyaline cartilage phenotype matrix scaffold for tissue engineering. Here, we reported a method using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride /N-hydroxysuccinimide (EDC/NHS) to achieve the grafting of chondroitin sulfate (CS) onto dLhCG, ultimately producing a tissue-engineered hyaline cartilage graft with the CS (dLhCG/CS). Young's modulus measurements revealed that the cross-linked scaffolds exhibited enhanced mechanical properties. We implanted the cross-linked dLhCG/CS scaffolds into the trochlear region of rat joints and evaluated their functionality through histological analysis and biomechanical tests. After 12 weeks, the dLhCG/CS scaffolds demonstrated excellent bioinductive activity comparable to dLhCG. The regenerated tissue effectively maintained a hyaline cartilage phenotype and exhibited similar mechanical properties, playing a crucial role in cartilage regeneration.

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