经硅学分析的鸡分离的Limosilactobacillus reuteri假想蛋白可诱导IL-2和IL-10。

IF 3.3 3区 医学 Q2 GENETICS & HEREDITY
Isaac Oluseun Adejumo
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引用次数: 0

摘要

乳酸菌(LAB)益生菌具有促进健康的作用,但其特性、安全性和功能机制尚不完全清楚。因此,本研究旨在表征鸡分离的Limosilactobacillus reuteri基因组中的一些假定蛋白,并揭示它们的IL-2和IL-10诱导潜力,以了解其免疫功能机制,从而实现可持续应用。对所选蛋白质的跨膜拓扑结构、亚细胞定位、IL-2和IL-10诱导能力以及IL-2和IL-10基因在不同条件下的表达进行了硅学分析。IL-2和IL-10诱导突变体采用SAS一般线性模型的单因素方差分析进行统计分析,统计显著性设定为P<0.05。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Hypothetical proteins of chicken-isolated Limosilactobacillus reuteri subjected to in silico analyses induce IL-2 and IL-10.

Lactic acid bacteria (LAB) probiotics are health-promoting but their characteristics, safety profile and functional mechanisms are not fully understood. Hence, this study aimed to characterize some hypothetical proteins of the chicken-isolated Limosilactobacillus reuteri genome and unravel their IL-2 and IL-10-inducing potential to understand mechanisms of their immunological functionality for sustainable applications. The selected proteins were subjected to in silico analyses for transmembrane topology, sub-cellular localization, IL-2 and IL-10-inducing ability and IL-2 and IL-10 gene expression across various conditions. IL-2 and IL-10-inducing mutants were statistically analyzed using a one-way analysis of variance of a general linear model of SAS and statistical significance was set at p < 0.05. The analyzed proteins are stable under a wide temperature range. All the hypothetical proteins are IL-2 and IL-10-inducing but QHPv.2.12, QHPv.2.13 and QHPv.2.15 are non-immunogenic. The evaluated mutants are IL-2 and IL-10-inducers but QHPv.2.13 and QHPv.2.15 are not immunogenic. This study sheds light on understanding the functional mechanisms of chicken-isolated L. reuteri and suggests it or its proteins as potent candidates for feed additive and therapeutic purposes.

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来源期刊
Genes and Nutrition
Genes and Nutrition 生物-遗传学
CiteScore
6.60
自引率
0.00%
发文量
14
审稿时长
6-12 weeks
期刊介绍: This journal examines the relationship between genetics and nutrition, with the ultimate goal of improving human health. It publishes original research articles and review articles on preclinical research data coming largely from animal, cell culture and other experimental models as well as critical evaluations of human experimental data to help deliver products with medically proven use.
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