亚洲旱地沙漠蝎 Mesobuthus przewalskii 染色体级基因组组装。

IF 3 2区 生物学 Q2 EVOLUTIONARY BIOLOGY
Yan-Kai Su, Ming-Hua Xiu, Hao-Yan Yang, Cheng-Min Shi
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引用次数: 0

摘要

蝎子是一类古老而富有魅力的节肢动物,在医学上具有重要意义,但该类动物仍然缺乏高质量的参考基因组。在这里,我们对塔克拉玛干沙漠特有的沙漠蝎子 Mesobuthus przewalskii 进行了全基因组测序。我们结合 PacBio HiFi 测序和 Hi-C 染色体构象捕获技术,为这种在医学上具有重要意义的蝎子生成了染色体水平、单体型解析和完整注释的基因组组装。该组配包括两个单倍型(分别为 1052.01 Mbp 和 1055.19 Mbp),达到了染色体水平的连续性和大于 98% 的 BUSCO 完整性。序列被锚定在 13 条染色体上,连片 N50 为 34.44 Mbp,支架 N50 为 81.43 Mbp。还提供了几个关键基因组特征和线粒体基因组组装。该基因组是蝎子目第五个但也是最完整的一个基因组。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A chromosome-level genome assembly for the desert scorpion Mesobuthus przewalskii from Asian drylands.

Scorpions are an ancient and charismatic group of arthropods with medical importance, but a high-quality reference genome for this group is still lacking. Here we perform whole-genome sequencing of Mesobuthus przewalskii, a desert scorpion endemic to the Taklimakan Desert. We combine PacBio HiFi sequencing and Hi-C chromosome conformation capturing to generate chromosomal-level, haplotype-resolved, and fully annotated genome assembly for this medically important scorpion. The assembly consists of two haplotypes (1052.01 Mbp and 1055.19 Mbp, respectively) reaching chromosome-level contiguity and >98% BUSCO completeness. Sequences were anchored in 13 chromosomes with a contig N50 of 34.44 Mbp and scaffold N50 of 81.43 Mbp. Several key genome features and the mitochondrial genome assembly were also provided. This genome represents the fifth but the most complete assembly for the order Scorpiones.

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来源期刊
Journal of Heredity
Journal of Heredity 生物-遗传学
CiteScore
5.20
自引率
6.50%
发文量
63
审稿时长
6-12 weeks
期刊介绍: Over the last 100 years, the Journal of Heredity has established and maintained a tradition of scholarly excellence in the publication of genetics research. Virtually every major figure in the field has contributed to the journal. Established in 1903, Journal of Heredity covers organismal genetics across a wide range of disciplines and taxa. Articles include such rapidly advancing fields as conservation genetics of endangered species, population structure and phylogeography, molecular evolution and speciation, molecular genetics of disease resistance in plants and animals, genetic biodiversity and relevant computer programs.
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