基于 H22 肿瘤小鼠模型的肝细胞癌潜在治疗靶点筛选方案。

IF 1.3 Q4 BIOCHEMICAL RESEARCH METHODS

STAR Protocols Pub Date : 2024-12-20 Epub Date: 2024-10-17 DOI:10.1016/j.xpro.2024.103193

Xiaoxiang Gao, Yinghui Qiu, Wei Liao, Chao Zhao

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引用次数: 0

摘要

调控与癌症相关的微RNA（miRNA）可能成为新一代癌症治疗方法。在此，我们介绍一种基于肝癌-22（H22）肿瘤小鼠的方案，以筛选治疗肝细胞癌（HCC）的潜在靶点。我们详细介绍了 H22 肿瘤小鼠的构建以及两种天然化合物--乳莼多糖（ULP）和 5-氟尿嘧啶（5FU）的治疗。我们进一步介绍了分离肿瘤组织进行 miRNA 测序以及发现和验证潜在的 HCC miRNA 基因靶点的过程。有关该方案使用和执行的完整细节，请参阅邱晓华等人的文章1。

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Protocol for screening potential targets for the treatment of hepatocellular carcinoma based on H22 tumor-bearing mouse model.

Regulating cancer-related microRNAs (miRNAs) may become a new generation of therapeutic modalities for cancer treatment. Here, we describe a protocol based on hepatoma-22 (H22) tumor-bearing mice to screen potential targets for treating hepatocellular carcinoma (HCC). We detail the construction of H22 tumor-bearing mice and treatment with two natural compounds, Ulva lactuca L. polysaccharide (ULP) and 5-fluorouracil (5FU). We further describe the isolation of the tumor tissues for miRNA sequencing and the discovery and validation of potential miRNA gene targets against HCC. For complete details on the use and execution of this protocol, please refer to Qiu et al.¹.

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