{"title":"使用差示扫描荧光测定法评估体外蛋白质聚氟烃基物质的规程。","authors":"Hannah M Starnes, Scott M Belcher","doi":"10.1016/j.xpro.2024.103386","DOIUrl":null,"url":null,"abstract":"<p><p>Per- and polyfluoroalkyl substances (PFAS) are ubiquitous synthetic chemicals that threaten public health, and serum albumin binding of PFAS represents one major variable influencing PFAS toxicokinetics. In this protocol, we describe a differential scanning fluorimetry (DSF) assay suitable for the rapid determination of the relative binding affinities of serum albumin proteins to different PFAS. Herein, we address common experimental challenges related to PFAS solubility constraints, the high background fluorescence of DSF with serum albumins, and the limitations of using DSF-derived dissociation constants (K<sub>D</sub>) to quantify PFAS-albumin interactions. For complete details on the use and execution of this protocol, please refer to Jackson et al.<sup>1</sup>.</p>","PeriodicalId":34214,"journal":{"name":"STAR Protocols","volume":"5 4","pages":"103386"},"PeriodicalIF":1.3000,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530897/pdf/","citationCount":"0","resultStr":"{\"title\":\"Protocol for evaluating protein-polyfluoroalkyl substances in vitro using differential scanning fluorimetry.\",\"authors\":\"Hannah M Starnes, Scott M Belcher\",\"doi\":\"10.1016/j.xpro.2024.103386\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Per- and polyfluoroalkyl substances (PFAS) are ubiquitous synthetic chemicals that threaten public health, and serum albumin binding of PFAS represents one major variable influencing PFAS toxicokinetics. In this protocol, we describe a differential scanning fluorimetry (DSF) assay suitable for the rapid determination of the relative binding affinities of serum albumin proteins to different PFAS. Herein, we address common experimental challenges related to PFAS solubility constraints, the high background fluorescence of DSF with serum albumins, and the limitations of using DSF-derived dissociation constants (K<sub>D</sub>) to quantify PFAS-albumin interactions. For complete details on the use and execution of this protocol, please refer to Jackson et al.<sup>1</sup>.</p>\",\"PeriodicalId\":34214,\"journal\":{\"name\":\"STAR Protocols\",\"volume\":\"5 4\",\"pages\":\"103386\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2024-12-20\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530897/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"STAR Protocols\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.xpro.2024.103386\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/10/15 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"STAR Protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.xpro.2024.103386","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/15 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
Protocol for evaluating protein-polyfluoroalkyl substances in vitro using differential scanning fluorimetry.
Per- and polyfluoroalkyl substances (PFAS) are ubiquitous synthetic chemicals that threaten public health, and serum albumin binding of PFAS represents one major variable influencing PFAS toxicokinetics. In this protocol, we describe a differential scanning fluorimetry (DSF) assay suitable for the rapid determination of the relative binding affinities of serum albumin proteins to different PFAS. Herein, we address common experimental challenges related to PFAS solubility constraints, the high background fluorescence of DSF with serum albumins, and the limitations of using DSF-derived dissociation constants (KD) to quantify PFAS-albumin interactions. For complete details on the use and execution of this protocol, please refer to Jackson et al.1.
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