基于 CRISPR 的血吸虫和相关扁形虫功能基因组学。

IF 7 1区医学 Q1 PARASITOLOGY

Trends in parasitology Pub Date : 2024-11-01 Epub Date: 2024-10-18 DOI:10.1016/j.pt.2024.09.010

Wannaporn Ittiprasert, Paul J Brindley

{"title":"基于 CRISPR 的血吸虫和相关扁形虫功能基因组学。","authors":"Wannaporn Ittiprasert, Paul J Brindley","doi":"10.1016/j.pt.2024.09.010","DOIUrl":null,"url":null,"abstract":"CRISPR genome editing is actively used for schistosomes and other flukes. The ability to genetically manipulate these flatworms enables deeper investigation of their (patho)biological nature. CRISPR gene knockout (KO) demonstrated that a liver fluke growth mediator contributes to disease progression. Genome safe harbor sites have been predicted in Schistosoma mansoni and targeted for transgene insertion. CRISPR-based diagnosis has been demonstrated for infection with schistosomes and Opisthorchis viverrini. This review charts the progress, and the state of play, and posits salient questions for the field to address. Derivation of heritably transgenic loss-of-function or gain-of-function lines is the next milestone.","PeriodicalId":23327,"journal":{"name":"Trends in parasitology","volume":" ","pages":"1016-1028"},"PeriodicalIF":7.0000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11560492/pdf/","citationCount":"0","resultStr":"{\"title\":\"CRISPR-based functional genomics for schistosomes and related flatworms.\",\"authors\":\"Wannaporn Ittiprasert, Paul J Brindley\",\"doi\":\"10.1016/j.pt.2024.09.010\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"CRISPR genome editing is actively used for schistosomes and other flukes. The ability to genetically manipulate these flatworms enables deeper investigation of their (patho)biological nature. CRISPR gene knockout (KO) demonstrated that a liver fluke growth mediator contributes to disease progression. Genome safe harbor sites have been predicted in Schistosoma mansoni and targeted for transgene insertion. CRISPR-based diagnosis has been demonstrated for infection with schistosomes and Opisthorchis viverrini. This review charts the progress, and the state of play, and posits salient questions for the field to address. Derivation of heritably transgenic loss-of-function or gain-of-function lines is the next milestone.\",\"PeriodicalId\":23327,\"journal\":{\"name\":\"Trends in parasitology\",\"volume\":\" \",\"pages\":\"1016-1028\"},\"PeriodicalIF\":7.0000,\"publicationDate\":\"2024-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11560492/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Trends in parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.pt.2024.09.010\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/10/18 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trends in parasitology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.pt.2024.09.010","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/10/18 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

CRISPR 基因组编辑技术被积极用于血吸虫和其他吸虫。对这些扁形虫进行基因操作的能力使人们能够更深入地研究它们的（病理）生物学特性。CRISPR基因敲除（KO）表明，一种肝吸虫生长介质有助于疾病的发展。曼氏血吸虫的基因组安全港位置已被预测出来，并成为转基因插入的目标。基于CRISPR技术的诊断已被证实可用于血吸虫和Opisthorchis viverrini的感染。本综述介绍了这一研究的进展和现状，并提出了该领域需要解决的突出问题。下一个里程碑是衍生出遗传性转基因功能缺失或功能增益品系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

CRISPR-based functional genomics for schistosomes and related flatworms.

CRISPR genome editing is actively used for schistosomes and other flukes. The ability to genetically manipulate these flatworms enables deeper investigation of their (patho)biological nature. CRISPR gene knockout (KO) demonstrated that a liver fluke growth mediator contributes to disease progression. Genome safe harbor sites have been predicted in Schistosoma mansoni and targeted for transgene insertion. CRISPR-based diagnosis has been demonstrated for infection with schistosomes and Opisthorchis viverrini. This review charts the progress, and the state of play, and posits salient questions for the field to address. Derivation of heritably transgenic loss-of-function or gain-of-function lines is the next milestone.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Trends in parasitology 医学-寄生虫学

CiteScore

14.00

自引率

3.10%

发文量

148

审稿时长

6-12 weeks

期刊介绍： Since its inception as Parasitology Today in 1985, Trends in Parasitology has evolved into a highly esteemed review journal of global significance, reflecting the importance of medical and veterinary parasites worldwide. The journal serves as a hub for communication among researchers across all disciplines of parasitology, encompassing endoparasites, ectoparasites, transmission vectors, and susceptible hosts. Each monthly issue of Trends in Parasitology offers authoritative, cutting-edge, and yet accessible review articles, providing a balanced and comprehensive overview, along with opinion pieces offering personal and novel perspectives. Additionally, the journal publishes a variety of short articles designed to inform and stimulate thoughts in a lively and widely-accessible manner. These include Science & Society (discussing the interface between parasitology and the general public), Spotlight (highlighting recently published research articles), Forum (presenting single-point hypotheses), Parasite/Vector of the Month (featuring a modular display of the selected species), Letter (providing responses to recent articles in Trends in Parasitology), and Trendstalk (conducting interviews). Please note that the journal exclusively publishes literature reviews based on published data, with systematic reviews, meta-analysis, and unpublished primary research falling outside our scope.