有机体在再生医学中的潜在用途。

IF 4.4 4区 医学 Q2 CELL & TISSUE ENGINEERING
Wahyunia L Septiana, Jeanne A Pawitan
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引用次数: 0

摘要

背景:体外细胞培养对研究人类疾病和发育至关重要。与传统的单层培养相比,用器官组织进行三维培养具有显著优势,能更准确地复制天然组织的结构和功能特征。这一进步增强了疾病建模、药物测试和再生医学应用。源自干细胞的类器官能以更贴切的方式模拟组织生理学。尽管它们前景广阔,但再生医学的临床应用目前还需要通过可重复性、可扩展性和成熟问题加以改进:本文概述了最近的类器官研究,重点是类器官的类型、来源、三维培养方法以及在再生医学中的应用。在PubMed/MEDLINE上对 "类器官 "和 "再生医学 "进行了文献综述,突出了过去十年间发表的相关研究,强调了人类来源的类器官及其再生功效,以及免费全文文章的可用性。该综述使用描述性数据(包括表格和文本)来说明器官组织在再生医学中的挑战和潜力:结果:从二维模型到三维模型的转变,尤其是有机体,极大地推动了体外研究的发展。这篇综述涵盖了十年来各种类型的类器官(如肝脏、胆管细胞、肠道、胰腺、心脏、大脑、胸腺和乳腺类器官)及其三维培养方法和应用的进展。报告探讨了成熟度、可扩展性和可重复性等关键问题,强调了标准化和改进生产技术的必要性,以促进再生医学更广泛的临床应用:成功的治疗需要更高的可扩展性和标准化。结论:成功的治疗需要更高的可扩展性和标准化。尽管存在障碍,器官组织在生物研究中仍有巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Potential Use of Organoids in Regenerative Medicine.

Background: In vitro cell culture is crucial for studying human diseases and development. Compared to traditional monolayer cultures, 3D culturing with organoids offers significant advantages by more accurately replicating natural tissues' structural and functional features. This advancement enhances disease modeling, drug testing, and regenerative medicine applications. Organoids, derived from stem cells, mimic tissue physiology in a more relevant manner. Despite their promise, the clinical use of regenerative medicine currently needs to be improved by reproducibility, scalability, and maturation issues.

Methods: This article overviews recent organoid research, focusing on their types, sources, 3D culturing methods, and applications in regenerative medicine. A literature review of "organoid" and "regenerative medicine" in PubMed/MEDLINE highlighted relevant studies published over the past decade, emphasizing human-sourced organoids and their regenerative benefits, as well as the availability of free full-text articles. The review uses descriptive data, including tables and text, to illustrate the challenges and potential of organoids in regenerative medicine.

Results: The transition from 2D to 3D models, particularly organoids, has significantly advanced in vitro research. This review covers a decade of progress in various organoid types-such as liver, cholangiocyte, intestinal, pancreatic, cardiac, brain, thymus, and mammary organoids-and their 3D culture methods and applications. It addresses critical issues of maturity, scalability, and reproducibility and underscores the need for standardization and improved production techniques to facilitate broader clinical applications in regenerative medicine.

Conclusions: Successful therapy requires increased scalability and standardization. Organoids have enormous potential in biological research, notwithstanding obstacles.

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来源期刊
Tissue engineering and regenerative medicine
Tissue engineering and regenerative medicine CELL & TISSUE ENGINEERING-ENGINEERING, BIOMEDICAL
CiteScore
6.80
自引率
5.60%
发文量
83
审稿时长
6-12 weeks
期刊介绍: Tissue Engineering and Regenerative Medicine (Tissue Eng Regen Med, TERM), the official journal of the Korean Tissue Engineering and Regenerative Medicine Society, is a publication dedicated to providing research- based solutions to issues related to human diseases. This journal publishes articles that report substantial information and original findings on tissue engineering, medical biomaterials, cells therapy, stem cell biology and regenerative medicine.
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