液体增稠剂、保存温度和时间对绵羊精子质量的影响

IF 1.6 3区农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Reproduction in Domestic Animals Pub Date : 2024-10-01 DOI:10.1111/rda.14653

E Díaz-Hernández, P Bóveda, C M Picazo, J A Laborda-Gomariz, M Serralle, M Ramón, R Gallego, V Montoro, O García-Álvarez, R Fernández-Santos, J J Garde, A J Soler

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引用次数: 0

摘要

在这项研究中，我们使用了含蛋黄和不含蛋黄的柠檬酸三柠檬酸果糖型稀释剂、不含果糖和含蛋黄的柠檬酸三柠檬酸型稀释剂以及商用稀释剂 Biladyl®，将稀释后的精液在 15 和 23°C 下保存不同时间（4、24、48 和 72 小时），评估了绵羊精子的质量。结果表明，含果糖和蛋黄的稀释剂对精液质量的影响最大。此外，与 15°C 温度（对照组）相比，23°C 温度下产生的 ROS 更高。此外，VCL 和具有完整顶体的精子百分比随着温度升高而降低。最后，精子质量在保存 24 小时后出现急剧下降，大多数评估参数都是如此。

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Influence of liquid extender, preservation temperature and time on the sheep sperm quality.

In this study, we evaluated sheep sperm quality after using Tris-citrate-fructose-based extender with and without egg yolk, a Tris-citrate without fructose and with egg yolk and the commercial extender Biladyl®, preserving diluted semen at 15 and 23°C for different times (4, 24, 48 and 72 h). The results showed that the diluents with fructose and egg yolk gave the best results of seminal quality. Moreover, the production of ROS was higher for the temperature of 23°C compared to the temperature of 15°C (control). In addition, VCL and the percentage of spermatozoa with intact acrosome decreased with temperatures of 23°C. Finally, a drastic decrease in sperm quality was observed after 24 hours of preservation for most of the parameters evaluated.

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来源期刊

Reproduction in Domestic Animals 农林科学-奶制品与动物科学

CiteScore

3.00

自引率

5.90%

发文量

238

审稿时长

4-8 weeks

期刊介绍： The journal offers comprehensive information concerning physiology, pathology, and biotechnology of reproduction. Topical results are currently published in original papers, reviews, and short communications with particular attention to investigations on practicable techniques. Carefully selected reports, e. g. on embryo transfer and associated biotechnologies, gene transfer, and spermatology provide a link between basic research and clinical application. The journal applies to breeders, veterinarians, and biologists, and is also of interest in human medicine. Interdisciplinary cooperation is documented in the proceedings of the joint annual meetings. Fields of interest: Animal reproduction and biotechnology with special regard to investigations on applied and clinical research.