加热和过氧化氢处理对脱矿皮质骨的骨诱导性的影响:制备肌腱/韧带修复支架的一种潜在方法。

IF 5.6 1区 医学 Q1 MATERIALS SCIENCE, BIOMATERIALS
Regenerative Biomaterials Pub Date : 2024-09-25 eCollection Date: 2024-01-01 DOI:10.1093/rb/rbae116
Shukun He, Ruonan Hu, Xuan Yao, Jing Cui, Huimin Liu, Min Zhu, Liangju Ning
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引用次数: 0

摘要

最近的研究表明,去矿物质皮质骨(DCB)可用于修复肌腱和韧带,如髌腱和前十字韧带(ACL)。过氧化氢(H2O2)已被证明能降低去骨皮质骨(DCB)的骨诱导性,而热处理也能降低去骨皮质骨(DCB)的骨诱导性。本研究的目的是:(i) 确定热处理是否会降低 DCB 的骨诱导性;(ii) 比较热处理和 H2O2 处理对 BMP-2 失活的效果。DCB 由 0.6 N 盐酸浸泡制备而成,DCB-H 和 DCB-HO 分别由热处理(70°C,8 小时)和 H2O2 处理(3% H2O2,8 小时)制备而成。通过扫描电子显微镜(SEM)、傅立叶变换红外光谱(FT-IR)和组织学染色观察了支架的表面形貌、元素分布和组织学结构。通过活/死细胞染色和细胞计数试剂盒-8(CCK-8)检测、实时聚合酶链反应(RT-PCR)、Western bolt(WB)分析、碱性磷酸酶活性(ALP)和茜素红 S(ARS)染色,评估了在支架上培养的 TDSCs 的活力和成骨分化情况。我们还利用大鼠肌肉植入支架的方法评估了热处理和 H2O2 处理对 DCB 骨诱导性的影响。结果表明,两种处理方法都能去除 DCB 内的 BMP-2 和骨钙素(OCN),DCB-H 和 DCB-HO 具有良好的细胞相容性,并能降低 TDSCs 的成骨分化。此外,体内研究结果表明,DCB-H 组和 DCB-HO 组的骨形成面积小于 DCB 组,DCB-HO 组的骨形成面积是三组中最小的。我们的研究表明,热处理可降低 DCB 的骨诱导性,而 H2O2 处理比热处理更有效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effects of heat and hydrogen peroxide treatment on the osteoinductivity of demineralized cortical bone: a potential method for preparing tendon/ligament repair scaffolds.

Recent studies have indicated that demineralized cortical bone (DCB) may be used to repair tendons and ligaments, such as the patellar tendon and anterior cruciate ligament (ACL). Hydrogen peroxide (H2O2) has been shown to reduce the osteoinductivity of DCB, and heat treatment may also decrease the osteoinductivity of DCB. The purpose of this study was (i) to determine whether heat treatment reduces the osteoinductivity of DCB and (ii) to compare the effectiveness of heat treatment and H2O2 treatment on BMP-2 inactivation. DCB was prepared by immersion in 0.6 N hydrochloric acid, and DCB-H and DCB-HO were prepared by heat treatment (70°C for 8 h) and H2O2 treatment (3% H2O2 for 8 h), respectively. The surface topographies, elemental distributions and histological structures of the scaffolds were observed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and histological staining. The viability and osteogenic differentiation of TDSCs cultured on the scaffolds were evaluated via live/dead cell staining and Cell Counting Kit-8 (CCK-8) testing, real-time polymerase chain reaction (RT-PCR) and western bolt (WB) analysis, alkaline phosphatase activity (ALP) and alizarin red S (ARS) staining. The intramuscular implantation of the scaffolds in rats was also used to evaluate the effect of heat treatment and H2O2 treatment on the osteoinductivity of DCB. Our results demonstrated that both treatments removed BMP-2 and osteocalcin (OCN) within the DCB and that DCB-H and DCB-HO had good cytocompatibility and reduced the osteogenic differentiation of TDSCs. Moreover, the in vivo results indicated that the DCB-H and DCB-HO groups had smaller areas of osteoid formation than did the DCB group, and the DCB-HO group had the smallest area among the three groups. Our study demonstrated that heat treatment could reduce the osteoinductivity of DCB, and that H2O2 treatment was more effective than heat treatment.

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来源期刊
Regenerative Biomaterials
Regenerative Biomaterials Materials Science-Biomaterials
CiteScore
7.90
自引率
16.40%
发文量
92
审稿时长
10 weeks
期刊介绍: Regenerative Biomaterials is an international, interdisciplinary, peer-reviewed journal publishing the latest advances in biomaterials and regenerative medicine. The journal provides a forum for the publication of original research papers, reviews, clinical case reports, and commentaries on the topics relevant to the development of advanced regenerative biomaterials concerning novel regenerative technologies and therapeutic approaches for the regeneration and repair of damaged tissues and organs. The interactions of biomaterials with cells and tissue, especially with stem cells, will be of particular focus.
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