Marisol Giustozzi , Santiago Nicolás Freytes , María Lorena Falcone Ferreyra , Pablo Cerdán , Paula Casati
{"title":"介体亚基 17 调节拟南芥植物的光照和黑暗反应。","authors":"Marisol Giustozzi , Santiago Nicolás Freytes , María Lorena Falcone Ferreyra , Pablo Cerdán , Paula Casati","doi":"10.1016/j.plantsci.2024.112285","DOIUrl":null,"url":null,"abstract":"<div><div>Mediator 17 (MED17) is part of the head of the Mediator complex, which regulates transcription initiation in different eukaryotic organisms, including plants. We have previously characterized MED17 roles in Arabidopsis plants exposed to UV-B radiation, revealing its involvement in various aspects of the DNA damage response after exposure. <em>med17</em> mutant plants showed altered <em>HY5</em> expression, which encodes a transcription factor with a central role in photomorphogenesis. Our results demonstrate that <em>med17</em> mutants show altered photomorphogenic responses and also to darkness, when compared to WT plants, and these differences could be due to altered expression of genes encoding key regulators of light and darkness signaling pathways, such as <em>HY5</em>, <em>COP1</em> and <em>PIF3</em>. Moreover, <em>med17</em> mutants exhibit transcriptome changes similar to those previously reported in plants exposed to red and blue light, as well as those previously described for photoreceptor mutants. Interestingly, <em>med17</em> and <em>hy5</em> mutants show a similar set of differentially expressed genes compared to WT plants, which suggests that both proteins may participate in a common light and dark-induced signaling pathways. Together, our data provides evidence that MED17 is an important regulator of the light and darkness responses in Arabidopsis.</div></div>","PeriodicalId":20273,"journal":{"name":"Plant Science","volume":null,"pages":null},"PeriodicalIF":4.2000,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Mediator subunit 17 regulates light and darkness responses in Arabidopsis plants\",\"authors\":\"Marisol Giustozzi , Santiago Nicolás Freytes , María Lorena Falcone Ferreyra , Pablo Cerdán , Paula Casati\",\"doi\":\"10.1016/j.plantsci.2024.112285\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Mediator 17 (MED17) is part of the head of the Mediator complex, which regulates transcription initiation in different eukaryotic organisms, including plants. We have previously characterized MED17 roles in Arabidopsis plants exposed to UV-B radiation, revealing its involvement in various aspects of the DNA damage response after exposure. <em>med17</em> mutant plants showed altered <em>HY5</em> expression, which encodes a transcription factor with a central role in photomorphogenesis. Our results demonstrate that <em>med17</em> mutants show altered photomorphogenic responses and also to darkness, when compared to WT plants, and these differences could be due to altered expression of genes encoding key regulators of light and darkness signaling pathways, such as <em>HY5</em>, <em>COP1</em> and <em>PIF3</em>. Moreover, <em>med17</em> mutants exhibit transcriptome changes similar to those previously reported in plants exposed to red and blue light, as well as those previously described for photoreceptor mutants. Interestingly, <em>med17</em> and <em>hy5</em> mutants show a similar set of differentially expressed genes compared to WT plants, which suggests that both proteins may participate in a common light and dark-induced signaling pathways. Together, our data provides evidence that MED17 is an important regulator of the light and darkness responses in Arabidopsis.</div></div>\",\"PeriodicalId\":20273,\"journal\":{\"name\":\"Plant Science\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2024-10-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Plant Science\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0168945224003121\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Science","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0168945224003121","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Mediator subunit 17 regulates light and darkness responses in Arabidopsis plants
Mediator 17 (MED17) is part of the head of the Mediator complex, which regulates transcription initiation in different eukaryotic organisms, including plants. We have previously characterized MED17 roles in Arabidopsis plants exposed to UV-B radiation, revealing its involvement in various aspects of the DNA damage response after exposure. med17 mutant plants showed altered HY5 expression, which encodes a transcription factor with a central role in photomorphogenesis. Our results demonstrate that med17 mutants show altered photomorphogenic responses and also to darkness, when compared to WT plants, and these differences could be due to altered expression of genes encoding key regulators of light and darkness signaling pathways, such as HY5, COP1 and PIF3. Moreover, med17 mutants exhibit transcriptome changes similar to those previously reported in plants exposed to red and blue light, as well as those previously described for photoreceptor mutants. Interestingly, med17 and hy5 mutants show a similar set of differentially expressed genes compared to WT plants, which suggests that both proteins may participate in a common light and dark-induced signaling pathways. Together, our data provides evidence that MED17 is an important regulator of the light and darkness responses in Arabidopsis.
期刊介绍:
Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment.
Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.