介体亚基 17 调节拟南芥植物的光照和黑暗反应。

IF 4.2 2区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Marisol Giustozzi , Santiago Nicolás Freytes , María Lorena Falcone Ferreyra , Pablo Cerdán , Paula Casati
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引用次数: 0

摘要

介导因子 17(MED17)是介导因子复合体头部的一部分,它在包括植物在内的不同真核生物体中调节转录起始。我们以前研究了 MED17 在暴露于 UV-B 辐射的拟南芥植物中的作用,发现它参与了暴露后 DNA 损伤反应的各个方面。med17 突变体植物显示出 HY5 表达的改变,而 HY5 编码的转录因子在光形态发生中起着核心作用。我们的研究结果表明,与 WT 植物相比,med17 突变体的光形态发生反应以及对黑暗的反应都发生了改变,这些差异可能是由于编码光和黑暗信号通路关键调控因子(如 HY5、COP1 和 PIF3)的基因表达发生了改变。此外,med17 突变体的转录组变化与之前报道的暴露于红光和蓝光的植物的转录组变化以及之前描述的感光突变体的转录组变化相似。有趣的是,与 WT 植物相比,med17 和 hy5 突变体显示出一组相似的差异表达基因,这表明这两种蛋白可能参与了共同的光和暗诱导信号通路。总之,我们的数据提供了证据,证明 MED17 是拟南芥光照和黑暗反应的重要调节因子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mediator subunit 17 regulates light and darkness responses in Arabidopsis plants
Mediator 17 (MED17) is part of the head of the Mediator complex, which regulates transcription initiation in different eukaryotic organisms, including plants. We have previously characterized MED17 roles in Arabidopsis plants exposed to UV-B radiation, revealing its involvement in various aspects of the DNA damage response after exposure. med17 mutant plants showed altered HY5 expression, which encodes a transcription factor with a central role in photomorphogenesis. Our results demonstrate that med17 mutants show altered photomorphogenic responses and also to darkness, when compared to WT plants, and these differences could be due to altered expression of genes encoding key regulators of light and darkness signaling pathways, such as HY5, COP1 and PIF3. Moreover, med17 mutants exhibit transcriptome changes similar to those previously reported in plants exposed to red and blue light, as well as those previously described for photoreceptor mutants. Interestingly, med17 and hy5 mutants show a similar set of differentially expressed genes compared to WT plants, which suggests that both proteins may participate in a common light and dark-induced signaling pathways. Together, our data provides evidence that MED17 is an important regulator of the light and darkness responses in Arabidopsis.
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来源期刊
Plant Science
Plant Science 生物-生化与分子生物学
CiteScore
9.10
自引率
1.90%
发文量
322
审稿时长
33 days
期刊介绍: Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.
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