拟南芥的发育和干旱逃逸反应受 AtPLC1 对脱落酸的调控。

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-10-22 DOI:10.1007/s00425-024-04554-4
Wei Wang, Yue Wang, Liping Luo, Jiaying Kou, Lulu Zhang, Chen Yang, Ning Yang
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引用次数: 0

摘要

主要结论AtPLC1 在植物的生长、发育和对干旱胁迫的反应中起着至关重要的作用。磷脂酰肌醇特异性磷脂酶 C(PI-PLC)水解底物,生成对植物生长、发育和胁迫响应至关重要的次级信使。干旱逃逸(DE)反应是植物在干旱条件下采用的一种适应性策略。在 plc1 中,花分生组织特异基因 APETALA 1 以及开花调控基因 FLOWERING LOCUS T 和 SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 的表达水平下调,而 FLOWERING LOCUS C 的表达水平上调。plc1flc 双突变体的开花时间早于野生型。转录组分析表明,差异表达基因(DEGs)的基因本体富含脱落酸(ABA)响应信号,京都基因和基因组百科全书分析表明,差异表达基因注释为植物激素信号通路。我们的实验表明,拟南芥中的 AtPLC1 受 ABA 上调。在 ABA 诱导和水分胁迫下,野生型植株表现出 DE 反应,而 plc1 的 DE 反应消失了。plc1中ABA信号通路转录因子ABA反应元件结合因子3(ABF3)和ABF4的表达水平下调。总之,我们的研究表明,AtPLC1 通过调节 ABA 信号通路转录因子 ABF3/ABF4 参与调控植物的生长和发育,并参与 DE 响应。该研究加深了我们对 AtPLC1 在植物生长发育和干旱胁迫中作用的理解,为进一步研究 DE 响应提供了理论基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development and drought escape response in Arabidopsis thaliana are regulated by AtPLC1 in response to abscisic acid.

Main conclusion: AtPLC1 plays a critical role in plant growth, development, and response to drought stress. Phosphoinositide-specific phospholipase C (PI-PLC) hydrolyzes substrates to generate secondary messengers crucial for plant growth, development, and stress responses. Drought escape (DE) response is an adaptive strategy that plants employ under drought conditions. The expression levels of the flower meristem-specific gene APETALA 1 and flowering regulatory genes FLOWERING LOCUS T and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 were downregulated in plc1, and FLOWERING LOCUS C was upregulated. The flowering time of the plc1flc double mutant was earlier than that of the wild type. Transcriptome analysis revealed that the Gene Ontology of differentially expressed genes (DEGs) was enriched in abscisic acid (ABA) response signaling, and Kyoto Encyclopedia of Genes and Genomes analysis revealed differential gene expression annotated to plant hormone signaling pathways. Our experiments show that AtPLC1 is upregulated by ABA in Arabidopsis. Under ABA induction and water stress, wild-type plants exhibit a DE response, and the DE response in plc1 disappears. Expression levels of ABA signaling pathway transcription factors ABA-responsive element-binding factors 3 (ABF3) and ABF4 were downregulated in plc1. In conclusion, our study suggests that AtPLC1 participates in regulating plant growth and development and participates in the DE response through the regulation of ABA signaling pathway transcription factors ABF3/ABF4. The study enhances our comprehension of the role of AtPLC1 in plant development and drought stress, providing a theoretical foundation for further investigation into DE responses.

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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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