Uma Kanta Chowra, Preetom Regon, Yuriko Kobayashi, Hiroyuki Koyama, Sanjib Kumar Panda
{"title":"Vigna mungo (L.) Hepper 中 Al3+ 毒性反应的特征和有机酸外流的分子机制。","authors":"Uma Kanta Chowra, Preetom Regon, Yuriko Kobayashi, Hiroyuki Koyama, Sanjib Kumar Panda","doi":"10.1007/s00425-024-04547-3","DOIUrl":null,"url":null,"abstract":"<p><p>Aluminium (Al<sup>3+</sup>) toxicity in acidic soils poses a significant challenge for crop cultivation and reduces crop productivity. The primary defense mechanism against Al<sup>3+</sup> toxicity involves the activation of organic acid secretion. In this study, responses of 9 Vigna mungo cultivars to Al<sup>3+</sup> toxicity were investigated, with a particular emphasis on the root system and crucial genes involved in Al<sup>3+</sup> tolerance using molecular cloning and expression analysis. Sensitive blackgram-KM2 cultivars exposed to 100-µM Al<sup>3+</sup> toxicity for 72 h exhibited a root-growth inhibition of approximately 66.17%. Significant loss of membrane integrity and structural deformative roots were found to be the primary symptoms of Al<sup>3+</sup> toxicity in blackgram. MATE (Multidrug and Toxic Compound Extrusion) and ALS3 (Aluminium Sensitive 3) genes were successfully cloned from a sensitive blackgram cv KM2 with phylogenetic analysis revealing their evolutionary relationship to Vigna radiata and Glycine max. The MATE gene is mainly localized in the plasma membrane, and highly expressed under Al<sup>3+</sup>, thus suggesting its role in transports of citrate-Al<sup>3+</sup> complexes, and detoxifying Al<sup>3+</sup> within plant cells. In addition, ALS3 was also induced under Al<sup>3+</sup> toxicity, which codes the UDP-glucose transporter and is required for the maintenance of ions homeostasis. In summary, this study highlights the understanding of Al<sup>3+</sup> toxicity and underlying molecular mechanisms linked to the efflux of organic acid in blackgram, ultimately aiding the framework for the development of strategies to enhance the resilience of blackgram and other pulse crops in Al-rich soils.</p>","PeriodicalId":20177,"journal":{"name":"Planta","volume":"260 5","pages":"116"},"PeriodicalIF":3.6000,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Characterization of Al<sup>3+</sup>-toxicity responses and molecular mechanisms underlying organic acid efflux in Vigna mungo (L.) Hepper.\",\"authors\":\"Uma Kanta Chowra, Preetom Regon, Yuriko Kobayashi, Hiroyuki Koyama, Sanjib Kumar Panda\",\"doi\":\"10.1007/s00425-024-04547-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Aluminium (Al<sup>3+</sup>) toxicity in acidic soils poses a significant challenge for crop cultivation and reduces crop productivity. The primary defense mechanism against Al<sup>3+</sup> toxicity involves the activation of organic acid secretion. In this study, responses of 9 Vigna mungo cultivars to Al<sup>3+</sup> toxicity were investigated, with a particular emphasis on the root system and crucial genes involved in Al<sup>3+</sup> tolerance using molecular cloning and expression analysis. Sensitive blackgram-KM2 cultivars exposed to 100-µM Al<sup>3+</sup> toxicity for 72 h exhibited a root-growth inhibition of approximately 66.17%. Significant loss of membrane integrity and structural deformative roots were found to be the primary symptoms of Al<sup>3+</sup> toxicity in blackgram. MATE (Multidrug and Toxic Compound Extrusion) and ALS3 (Aluminium Sensitive 3) genes were successfully cloned from a sensitive blackgram cv KM2 with phylogenetic analysis revealing their evolutionary relationship to Vigna radiata and Glycine max. The MATE gene is mainly localized in the plasma membrane, and highly expressed under Al<sup>3+</sup>, thus suggesting its role in transports of citrate-Al<sup>3+</sup> complexes, and detoxifying Al<sup>3+</sup> within plant cells. In addition, ALS3 was also induced under Al<sup>3+</sup> toxicity, which codes the UDP-glucose transporter and is required for the maintenance of ions homeostasis. In summary, this study highlights the understanding of Al<sup>3+</sup> toxicity and underlying molecular mechanisms linked to the efflux of organic acid in blackgram, ultimately aiding the framework for the development of strategies to enhance the resilience of blackgram and other pulse crops in Al-rich soils.</p>\",\"PeriodicalId\":20177,\"journal\":{\"name\":\"Planta\",\"volume\":\"260 5\",\"pages\":\"116\"},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2024-10-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Planta\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s00425-024-04547-3\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Planta","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00425-024-04547-3","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
Characterization of Al3+-toxicity responses and molecular mechanisms underlying organic acid efflux in Vigna mungo (L.) Hepper.
Aluminium (Al3+) toxicity in acidic soils poses a significant challenge for crop cultivation and reduces crop productivity. The primary defense mechanism against Al3+ toxicity involves the activation of organic acid secretion. In this study, responses of 9 Vigna mungo cultivars to Al3+ toxicity were investigated, with a particular emphasis on the root system and crucial genes involved in Al3+ tolerance using molecular cloning and expression analysis. Sensitive blackgram-KM2 cultivars exposed to 100-µM Al3+ toxicity for 72 h exhibited a root-growth inhibition of approximately 66.17%. Significant loss of membrane integrity and structural deformative roots were found to be the primary symptoms of Al3+ toxicity in blackgram. MATE (Multidrug and Toxic Compound Extrusion) and ALS3 (Aluminium Sensitive 3) genes were successfully cloned from a sensitive blackgram cv KM2 with phylogenetic analysis revealing their evolutionary relationship to Vigna radiata and Glycine max. The MATE gene is mainly localized in the plasma membrane, and highly expressed under Al3+, thus suggesting its role in transports of citrate-Al3+ complexes, and detoxifying Al3+ within plant cells. In addition, ALS3 was also induced under Al3+ toxicity, which codes the UDP-glucose transporter and is required for the maintenance of ions homeostasis. In summary, this study highlights the understanding of Al3+ toxicity and underlying molecular mechanisms linked to the efflux of organic acid in blackgram, ultimately aiding the framework for the development of strategies to enhance the resilience of blackgram and other pulse crops in Al-rich soils.
期刊介绍:
Planta publishes timely and substantial articles on all aspects of plant biology.
We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.