Vigna mungo (L.) Hepper 中 Al3+ 毒性反应的特征和有机酸外流的分子机制。

IF 3.6 3区 生物学 Q1 PLANT SCIENCES
Planta Pub Date : 2024-10-14 DOI:10.1007/s00425-024-04547-3
Uma Kanta Chowra, Preetom Regon, Yuriko Kobayashi, Hiroyuki Koyama, Sanjib Kumar Panda
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引用次数: 0

摘要

酸性土壤中的铝(Al3+)毒性给作物栽培带来了巨大挑战,并降低了作物产量。针对 Al3+ 毒性的主要防御机制包括激活有机酸分泌。本研究利用分子克隆和表达分析方法,研究了 9 个 Vigna mungo 栽培品种对 Al3+ 毒性的反应,重点是根系和参与 Al3+ 耐受的关键基因。敏感的黑芒果-KM2 栽培品种在暴露于 100-µM Al3+ 毒性 72 小时后,根系生长受到约 66.17% 的抑制。研究发现,膜完整性的显著丧失和根部结构的变形是黑鲩受 Al3+ 毒害的主要症状。从敏感的黑鲩品种 KM2 中成功克隆了 MATE(多药和有毒化合物挤出)和 ALS3(铝敏感性 3)基因,系统进化分析表明了它们与 Vigna radiata 和 Glycine max 的进化关系。MATE 基因主要定位于质膜,在 Al3+ 条件下高度表达,这表明它在柠檬酸盐-Al3+ 复合物的转运和植物细胞内 Al3+ 的解毒中发挥作用。此外,ALS3也在Al3+毒性下被诱导,它编码UDP-葡萄糖转运体,是维持离子平衡所必需的。总之,本研究强调了对 Al3+ 毒性以及与黑穗醋栗有机酸外流有关的潜在分子机制的认识,最终有助于制定战略框架,以提高黑穗醋栗和其他脉冲作物在富含 Al 的土壤中的抗逆性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of Al3+-toxicity responses and molecular mechanisms underlying organic acid efflux in Vigna mungo (L.) Hepper.

Aluminium (Al3+) toxicity in acidic soils poses a significant challenge for crop cultivation and reduces crop productivity. The primary defense mechanism against Al3+ toxicity involves the activation of organic acid secretion. In this study, responses of 9 Vigna mungo cultivars to Al3+ toxicity were investigated, with a particular emphasis on the root system and crucial genes involved in Al3+ tolerance using molecular cloning and expression analysis. Sensitive blackgram-KM2 cultivars exposed to 100-µM Al3+ toxicity for 72 h exhibited a root-growth inhibition of approximately 66.17%. Significant loss of membrane integrity and structural deformative roots were found to be the primary symptoms of Al3+ toxicity in blackgram. MATE (Multidrug and Toxic Compound Extrusion) and ALS3 (Aluminium Sensitive 3) genes were successfully cloned from a sensitive blackgram cv KM2 with phylogenetic analysis revealing their evolutionary relationship to Vigna radiata and Glycine max. The MATE gene is mainly localized in the plasma membrane, and highly expressed under Al3+, thus suggesting its role in transports of citrate-Al3+ complexes, and detoxifying Al3+ within plant cells. In addition, ALS3 was also induced under Al3+ toxicity, which codes the UDP-glucose transporter and is required for the maintenance of ions homeostasis. In summary, this study highlights the understanding of Al3+ toxicity and underlying molecular mechanisms linked to the efflux of organic acid in blackgram, ultimately aiding the framework for the development of strategies to enhance the resilience of blackgram and other pulse crops in Al-rich soils.

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来源期刊
Planta
Planta 生物-植物科学
CiteScore
7.20
自引率
2.30%
发文量
217
审稿时长
2.3 months
期刊介绍: Planta publishes timely and substantial articles on all aspects of plant biology. We welcome original research papers on any plant species. Areas of interest include biochemistry, bioenergy, biotechnology, cell biology, development, ecological and environmental physiology, growth, metabolism, morphogenesis, molecular biology, new methods, physiology, plant-microbe interactions, structural biology, and systems biology.
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