洞察药用兰花金钗石斛和欧石斛多糖和生物碱合成的差异

IF 5.4 2区 生物学 Q1 PLANT SCIENCES
Yu-Wen Zhang, Yu-Cen Shi, Wei Huang, Shi-Bao Zhang
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引用次数: 0

摘要

金钗石斛和欧石斛都是中国广泛使用的药用植物,它们的主要药用成分分别是生物碱和多糖。目前尚不清楚这两种亲缘关系很近的兰科植物为什么会合成和积累不同的化学成分。在此,我们通过转录组和代谢组分析,研究了金钗兰和兜兰在不同生长阶段多糖和生物碱生物合成的分子机制。在这两个物种的幼年期和成熟期,共鉴定出 1267 种代谢物。金钗草在从幼苗向成熟植株过渡的过程中积累了大量生物碱、苯类化合物/苯丙类化合物、黄酮类化合物和萜类化合物。相比之下,D. officinale 只积累了少量的这些代谢物,而没有黄酮类化合物。多糖含量与差异表达基因的相关性分析表明,GH1、GH3和GH9的差异表达可能与两种铁皮石斛多糖含量的差异有关。同时,金钗石斛生物碱的主要成分石斛碱在生物合成过程中的差异也涉及到 HMGCR、DXR、DXS、ISPH 和 8 个 CYP450s 的差异表达。这些发现为了解铁皮石斛主要药用成分的生物合成机制提供了新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Insights into the Differences in Polysaccharide and Alkaloid Biosynthesis in the Medicinal Orchids Dendrobium nobile and D. officinale.

Both Dendrobium nobile and D. officinale are widely used medicinal plants in China and their major medicinal components are alkaloids and polysaccharides, respectively. It is still unclear why these two closely related orchids synthesize and accumulate different chemical components. Here, we investigated the molecular mechanisms underlying polysaccharide and alkaloid biosynthesis in D. nobile and D. officinale through transcriptome and metabolomic analysis at different growth stages. A total of 1267 metabolites were identified in the juvenile and mature stages of the two species. D. nobile accumulated a large number of alkaloids, benzenoids/phenylpropanoids, flavonoids, and terpenoids during the transition from juvenile to mature plants. In contrast, D. officinale accumulated a small number of those metabolites and an absence of flavonoids. The correlation analysis of polysaccharide contents with the differentially expressed genes suggested that the differential expression of GH1, GH3, and GH9 might be related to the difference in polysaccharide contents between the two Dendrobium species. Meanwhile, the difference in the biosynthesis of dendrobine, the main component of alkaloids in D. nobile, was involved in the differential expression of HMGCR, DXR, DXS, ISPH and eight CYP450s. These findings provided new insights into understanding the biosynthetic mechanisms of the main medicinal components in Dendrobium species.

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来源期刊
Physiologia plantarum
Physiologia plantarum 生物-植物科学
CiteScore
11.00
自引率
3.10%
发文量
224
审稿时长
3.9 months
期刊介绍: Physiologia Plantarum is an international journal committed to publishing the best full-length original research papers that advance our understanding of primary mechanisms of plant development, growth and productivity as well as plant interactions with the biotic and abiotic environment. All organisational levels of experimental plant biology – from molecular and cell biology, biochemistry and biophysics to ecophysiology and global change biology – fall within the scope of the journal. The content is distributed between 5 main subject areas supervised by Subject Editors specialised in the respective domain: (1) biochemistry and metabolism, (2) ecophysiology, stress and adaptation, (3) uptake, transport and assimilation, (4) development, growth and differentiation, (5) photobiology and photosynthesis.
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